不同方法提取PAHs高效降解菌EPS的特性

生态学杂志

不同方法提取PAHs高效降解菌EPS的特性

张宇¹,梁彦秋¹,贾春云^2**,姜春阳³,焦阳¹

（¹沈阳化工大学，沈阳 110142； ^2中国科学院沈阳应用生态研究所污染生态与环境工程重点实验室，沈阳 110016； ^3沈阳理工大学，沈阳 110168）

出版日期:2014-04-10 发布日期:2014-04-10

Characteristics of EPS extracted from a high efficient PAHs-degrading bacterium by different methods.

ZHANG Yu¹, LIANG Yan-qiu¹, JIA Chun-yun^2**, JIANG Chun-yang³, JIAO Yang^1

(¹Shenyang University of Chemical Technology, Shenyang 110142, China; ²Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China; ^3Shenyang Ligong University, Shenyang 110168, China)

Online:2014-04-10 Published:2014-04-10

摘要/Abstract

摘要：

采用阳离子交换树脂法、超声法、超声阳离子交换树脂法、加热法4种方法提取一株PAHs高效降解菌的胞外聚合物（EPS），并结合红外光谱、三维荧光光谱、透射电子显微镜对不同方法提取的EPS进行特性分析。结果表明，加热法得到的EPS中蛋白质和多糖的总含量最大为328.36 mg·L^-1；4种方法提取EPS的红外光谱谱线均出现C(N)═O、N—H(蛋白质)和O—H（多聚糖）强峰，其中加热法提取EPS谱图中C(N)═O和N—H基团的吸收峰最宽、强度最大；三维荧光光谱分析结果表明，加热法提取EPS的2个类蛋白峰强度最大，分别为13.8、118.2 a.u.，而其他3种方法提取EPS的类蛋白峰强度较弱、类腐殖酸和类富里酸峰较强；透射电子显微镜检测结果表明，加热法细胞体破坏程度最小且胞内物质没有溶出现象。综合考虑4种方法提取EPS特性，加热法提取效率较高且温和，是比较理想的EPS提取方法。

关键词: 种群结构, 点格局分析, 种间关联

Abstract: Extracellular polymeric substances (EPSs) of a high efficient PAHs-degrading bacterium strain were extracted by four methods including cation exchange resin method, ultrasonic method, ultrasoniccation exchange resin method and heating method, and the properties of EPSs extracted by the four methods were analyzed by infrared spectrum, threedimensional fluorescence spectrum and transmission electron microscope. The maximum total content of protein and polysaccharide in EPSs by the heating method was 328.36 mg·L^-1. The adsorption peaks of C(N)═O, N―H (protein) and O―H (polysaccharides) were all present on the infrared spectrum of EPSs obtained by the four methods, and the peaks of C(N)═O and N―H on the spectra of EPSs extracted by the heating method were the widest and strongest. The threedimensional fluorescence spectrum analysis showed that the intensities of two proteinoid peaks in EPSs obtained by the heating method were maximum, being 13.8 and 118.2 a.u., respectively, while the proteinoid peak was weaker and the humiclike and fulviclike acids were stronger in EPSs obtained by the other three methods. Transmission electron microscopy analysis showed that the damage of bacterial cell was least and no intracellular substances dissolved out during the process of heating. According to the characteristics of the EPSs obtained by the four methods, the heating method was considered as the better EPS extraction method due to its highest efficiency and moderate impact on bacterial cell.

Key words: population structure, interspecific association, point pattern analysis

张宇1,梁彦秋1,贾春云2**,姜春阳3,焦阳1. 不同方法提取PAHs高效降解菌EPS的特性[J]. 生态学杂志.

ZHANG Yu1, LIANG Yan-qiu1, JIA Chun-yun2**, JIANG Chun-yang3, JIAO Yang1. Characteristics of EPS extracted from a high efficient PAHs-degrading bacterium by different methods.[J]. cje.

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