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药用红花幼苗对盐胁迫的生理响应机制

韩宇,生艳菲,罗茜,孙希超,王长海,赵耕毛**   

  1. (江苏省海洋生物学重点实验室, 南京农业大学资源与环境科学学院, 南京 210095)
  • 出版日期:2014-07-10 发布日期:2014-07-10

Physiological mechanism of Carthamus tinctorius L. seedlings in response to salt stress.

HAN Yu, SHENG Yan-fei, LUO Xi, SUN Xi-chao, WANG Chang-hai, ZHAO Geng-mao**   

  1. (College of Natural Resources and Environmental Science, Key Laboratory of Marine Biology, Nanjing Agricultural University, Nanjing 210095, China)
  • Online:2014-07-10 Published:2014-07-10

摘要: 以红花为材料,研究了不同浓度NaCl(0、50、100和150 mmol·L-1)胁迫对红花幼苗叶片中的可溶性糖(SS)、可溶性蛋白(SP)及抗氧化酶系(T-AOC、SOD、CAT)的影响。结果表明:盐分对红花幼苗生长的抑制作用随NaCl浓度增加而加剧,且对地上部分的抑制作用大于根部。胁迫初期(10 d),各处理红花幼苗中的T-AOC的活性没有明显变化;但高盐胁迫显著提高了CAT的活性。与对照相比,盐胁迫明显提高了红花中的SS、SP的含量及SOD的活性,但各盐处理之间没有明显差异。胁迫中期(20 d),各处理间SOD的活性无明显变化;SS的含量随盐浓度的增加而明显增加,且处理间差异显著;SP的含量和CAT、T-AOC的活性与对照相比均有所增加。盐胁迫后期(30 d),SP和SS的含量在叶片中有积累的趋势,但T-AOC和SOD却相反,其活性较对照处理均有所下降,而盐处理的CAT的活性较对照处理无明显差异。因此,供试红花幼苗在盐胁迫初期主要是通过合成渗透调节物质和活性氧清除机制共同作用来抵御盐分胁迫,随着胁迫时间的延长则主要通过合成渗透调节物质来抵御盐害,其中可溶性糖对盐浓度的响应起到关键作用。

关键词: 水稻, 非生物胁迫, 颖花不育, 花粉

Abstract: A hydroponic experiment was conducted to investigate the physiological mechanism of safflower (Carthamus tinctorius) in response to salt stress. Four treatments with various salt concentrations (0, 50, 100 and 150 mmol·L-1 NaCl) were employed and arranged by a completely randomized design with three replications. The results showed that the growth of safflower seedlings was significantly inhibited with the increasing salinity of hydroponic solution, while the effect of salt stress on the roots was less than that on the aerial parts. After a duration of 10 days salt stress, the SOD activity in safflower leaves under salt stress was significantly increased as compared with CK; however, TAOC activity remained unchanged. The contents of soluble protein and sugar among the salt treatments were not significantly different, but significantly increased in comparison with CK. When the safflower was saltstressed for 20 days, the SOD activity in leaves remained unchanged, but the CAT and T-AOC activities increased greatly as compared with CK. Also, the soluble sugar and protein contents greatly increased with increasing the salinity of hydroponic solutions. At 30 days of salt stress, the soluble sugar and protein were markedly accumulated in leaves of safflower. The activities of T-AOC and SOD were significantly decreased as compared with that of CK, while the CAT activity remained unchanged. Therefore, the safflower withstood the salt stress mainly through increasing the levels of osmoregulation substances and active oxygen scavenging capacity at the seedling stage, and the inductive increase of soluble sugar might play a key role.

Key words: pollen, abiotic stress, floret sterility, rice