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绿木霉(Trichoderma virens)T43对四种重要林木病原菌的抑制效果及抑菌机理

尹大川1,邓勋2,Ilan CHET3,宋瑞清1**   

  1. (1东北林业大学林学院, 哈尔滨 150040; 2黑龙江省林科院森林保护研究所, 哈尔滨 150040; 3以色列耶路撒冷希伯来大学农学院, 以色列 76100)
  • 出版日期:2014-07-10 发布日期:2014-07-10

Inhibiting effect and mechanism of Trichoderma virens T43 on four major species of forest pathogen.

YIN Da-chuan1, DENG Xun2, Ilan CHET3, SONG Rui-qing1**   

  1. (1Forestry School, Northeast Forestry University, Harbin 150040; 2Forestry Protection Institute of Heilongjiang Forestry Academy, Harbin 150040; 3Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, Israel 76100)
  • Online:2014-07-10 Published:2014-07-10

摘要:

通过对峙培养和非挥发性代谢产物对病原菌生长的抑制作用,开展了木霉菌株T43及其抑菌活性物质抑制樟子松枯梢病、杨树烂皮病和苗木立枯病3种病害的4种病原菌效果的研究。从菌株T43发酵液乙酸乙酯提取物对各病原菌的3种保护酶(CAT、POD和SOD)活力影响和对细胞渗透调节物质(可溶性蛋白、可溶性糖和丙二醛)的含量影响,探讨了提取物对病原菌的抑菌机理。结果表明:木霉菌株T43及其代谢产物对供试林木病原菌均有明显的抑制作用,菌株T43发酵液乙酸乙酯提取物对病原菌3种保护酶活性均有不同程度的影响,可导致病原菌3种保护酶活性降低,尤其是SOD活性下降的最为显著。该提取物可以显著降低菌丝体可溶性糖和蛋白含量,提高丙二醛含量,破坏细胞的完整性,这可能是绿木霉T43发酵液乙酸乙酯提取物抑制病原菌生长的机理之一。
 

关键词: 非线性混合模型, 杉木人工林, 林分变量, 树高-胸径模型

Abstract: Antagonistic culture and growth inhibition were used to assess the inhibiting effects of Trichoderma virens T43 on S-phaeropsis sapinea, Cytospora chrysosperma, Rhizoctonia solani and Fusarium oxysporum. The impacts of ethyl acetate extract of T. virens T43 fermented liquid on the protective enzymes (CAT, POD and SOD) and osmotic adjustment substances (soluble protein, soluble sugar and MDA) of pathogens were used to analyze the inhibition mechanism of T43 on the pathogens. The results showed that T43 and its metabolites significantly inhibited the pathogens. The ethyl acetate extract reduced the activities of protective enzymes in pathogens, with SOD activity decreasing most significantly. The extract also significantly reduced the soluble sugar and protein contents, and increased the MDA content in pathogens, hence destroying cell integrity. This may be one of the inhibiting mechanisms of ethyl acetate extract of fermented liquid of T43 on the growth of pathogens.

Key words: Chinese fir plantation, height-diameter model, nonlinear mixed-effect model, stand variables