欢迎访问《生态学杂志》官方网站,今天是 分享到:

生态学杂志

• 研究报告 • 上一篇    下一篇

内生真菌重组漆酶rLACB3修复花生连作土壤

朱虹1,2,王宏伟2,杜威2,戴传超2**   

  1. (1南京师范大学教师教育学院, 南京 210023; 2南京师范大学生命科学学院, 江苏省微生物资源产业化工程技术研究中心, 江苏省微生物与功能基因组学重点实验室, 南京 210023)
  • 出版日期:2014-07-10 发布日期:2014-07-10

Potential role of the endopytic fungus laccase rLACB3 in the bioremediation of peanut continuous cropping soil.

ZHU Hong1,2, WANG Hong-wei2, DU Wei2, DAI Chuan-chao2**   

  1. (1College of Teacher Education, Nanjing Normal University, Nanjing 210023, China; 2Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China)
  • Online:2014-07-10 Published:2014-07-10

摘要: 土壤中酚酸类物质的积累是导致花生连作障碍的主要原因之一,真菌漆酶可以有效地转化酚酸类物质,但还没有报道将漆酶直接应用于连作土壤的修复。本研究使用盆钵试验研究了不同浓度的漆酶rLACB3对连作土壤修复的效果。处理30 d后,施加500 U·kg-1漆酶处理的修复效果优于20和100 U·kg-1。在花生根际土壤中,500 U·kg-1漆酶处理的可培养细菌、放线菌、固氮菌数量和对照相比分别提高33.0%、37.7%和30.2%。使用变性梯度凝胶电泳(DGGE)分析根际土壤微生物区系表明,500 U·kg-1漆酶处理的细菌、真菌和固氮菌的Shannon多样性指数比对照分别提高9.0%、17.3%和14.8%。根际土壤中3种酚酸物质香豆酸、4-羟基苯甲酸和香草酸,500 U·kg-1漆酶处理比对照分别减少41.2%、43.8%和35.9%。花生生物量和结瘤数量,500 U·kg-1漆酶处理比对照分别增加17.9%和17.4%。综上表明,内生真菌重组漆酶rLACB3在连作土壤修复中具有较好的应用潜力。

关键词: 活塞流, 苹果园, 染色示踪, 黄土塬区, 入渗, 根系

Abstract: The accumulation of phenolic acids in soil is one of the main hindrances to continuous peanut cropping. Although laccases secreted by fungi can efficiently transform phenolic acids, the application of laccases in the bioremediation of continuously cropped soil has not been investigated. Therefore, a pot experiment was carried out to study the effect of adding different concentrations of laccase rLACB3 on a continuously cropped peanut soil. After 30 days, the 500 U·kg-1 laccase treatment was superior to those with the 20 and 100 U·kg-1 treatments in soil bioremediation. The populations of bacteria, fungi and Azotobacter in the rhizospheric soil of the 500 U·kg-1 treatment increased by 33.0%, 37.7% and 30.2% respectively, compared with the control. The rhizospheric soil microbial community was analyzed by denaturing gradient gel electrophoresis (DGGE), showing that the Shannon’s indexes of culturable bacteria, diazotrophic bacteria and azotobacter of the 500 U·kg-1 treatment increased by 9.0%, 17.3% and 14.8%, respectively compared with the control. The 500 U·kg-1 laccase treatment also had 41.2%, 43.8% and 35.9% lower levels of the phenolic acids, coumaric acid, 4hydroxybenzoic acid and vanillic acid, respectively compared with the control. The biomass and the number of nodules of the 500 U·kg-1 treatment increased by 17.9% and 17.4%, respectively compared with the control. These results indicated that rLACB3 was a potential candidate for the remediation of the continuously cropped peanut soil.

Key words: infiltration, dye tracing, piston flow, Loess Tableland, apple orchard, root