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西芹腐根浸提液作用后黄瓜枯萎病菌弱毒菌株的筛选

李杰,王勇,高晓敏,钱程,云兴福**   

  1. (1内蒙古农业大学农学院, 呼和浩特 010019)
  • 出版日期:2014-10-10 发布日期:2014-10-10

Screening of virulence-attenuated strains for Fusarium oxysporum f. sp. cucumerinum treated by extracts of the rotten root of the parsley.

LI Jie, WANG Yong, GAO Xiao-min, QIAN Cheng, YUN Xing-fu**   

  1. (College of Agriculture, Inner Mongolia Agricultural University, Hohhot 010019, China)
  • Online:2014-10-10 Published:2014-10-10

摘要:

在培养基条件下用西芹腐根乙醇、丙酮和蒸馏水浸提液,连续5代处理黄瓜枯萎病菌,研究其对黄瓜枯萎病菌的化感作用及温室人工接种致病力变化情况,以筛选黄瓜枯萎病菌的弱毒菌株。结果表明,腐根乙醇浸提液处理的黄瓜枯萎病菌菌落直径每一代在培养144 h时的化感作用效果分别是55.5%、45.5%、49.8%、53.7%、44.0%,与对照差异极显著,黄瓜枯萎病病情指数由第1代的43.0%降至第5代4.2%。腐根丙酮浸提液处理菌落直径每一代在培养144 h时的化感作用效果分别是23.1%、28.2%、24.3%、23.3%、19.3%,与对照差异极显著,病情指数由35.0%降至3.3%;腐根蒸馏水浸提液处理菌落直径每一代在培养144 h时的化感作用效果分别是9.7%、5.1%、7.4%、3.3%、2.8%,与对照差异不显著,病情指数由57.0%降至19.2%。据此确定第5代的腐根乙醇和丙酮浸提液处理的黄瓜枯萎病菌为弱毒菌株,其致病力稳定性均表现为良好。
 

关键词: 稳定同位素, 食物网, 累积, 重金属, 大亚湾

Abstract: In order to select the virulence-attenuated strains for Fusarium oxysporum f. sp. cucumerinum, the allelopathy and changes of pathogenicity of cucumber plants were detected after treatment by different parsley rotten root extracts on F. oxysporum f. sp. cucumerinum. The F. oxysporum f. sp. cucumerinum were treated from the first generation to fifth generation with parsley rotten roots extracted by ethanol, acetone and aqueous respectively in medium conditions. The results showed that the allelopathy effects of parsley rotten root ethanol extracts of each generation after 144 h treatment were 55.5%, 45.5%, 49.8%, 53.7%, and 44.0% respectively, and the pathogenicity dropped from 43.0% to 4.2% from the first generation to fifth generation. The allelopathy effects of acetone extract treatments were 23.1%, 28.2%, 24.3%, 23.3%, and 19.3% for the first to fifth generation respectively, and the disease index pathogenicity dropped from 35.0% to 3.3%. Both ethanol and acetone treatments had significant difference compared with their corresponding control. The allelopathy effects of aqueous extract treatments were 9.7%, 5.1%, 7.4%, 3.3%, and 2.8% for the first to fifth generation after 144 h treatment, showing no significant difference with their corresponding control, and the disease index dropped from 57.0% to 19.2%. Therefore, it was considered that virulenceattenuated F. oxysporum f. sp. cucumerinum strains could be screened after 5-generation treatment with ethanol or acetone extracts of parsley rotten roots, and they showed a good pathogenicity stability in cucumber plants.

Key words: stable isotopes, heavy metal, food web, Daya Bay, bioaccumulation