DGGE法初步解析茯砖茶发花过程中真菌群落结构

生态学杂志

DGGE法初步解析茯砖茶发花过程中真菌群落结构

刘石泉^1,2,赵运林^1**,胡治远¹

(¹ 湖南城市学院化学与环境工程学院，湖南益阳 413000； ^2 湖南农业大学生物科学与技术学院，长沙 410128)

出版日期:2014-10-10 发布日期:2014-10-10

Analysis of fungal community structure during the Fahua-fermentation process of Fuzhuan Brick Tea by DGGE technology.

LIU Shi-quan^1,2， ZHAO Yun-lin^1**， HU Zhi-yuan^1

(¹College of Chemistry and Environmental Engineering, Hunan City University, Yiyang 413000, Hunan, China; ^2College of Biology and Technology, Hunan Agricultural University, Changsha 410128, China)

Online:2014-10-10 Published:2014-10-10

摘要/Abstract

摘要：

为研究茯砖茶发花过程中真菌群落结构和种类，对发花过程中不同时段真菌落18S rDNA高变区进行扩增，并对真菌变性梯度凝胶电泳(DGGE)图谱中条带进行克隆、测序和序列比对分析。结果表明：茯砖茶发花前后时段其优势菌不同，以发花过程的第10天为临界点，0～8 d和10～14 d，前后存在2个差异较大的真菌优势种群结构的演变，10天前以好干性酵母菌、毕赤酵母、假丝酵母为优势菌，10 d后以阿姆斯特丹散囊菌、好干性酵母菌为优势菌；18S rDNA高变区比对结果表明，茯砖茶发花过程中有好干性酵母、汉逊德巴利酵母、酿酒酵母、假丝酵母菌、热带假丝酵母、路德酵母、毕赤酵母、牧草红酵母、隐球酵母、阿姆斯特丹散囊菌、曲霉、毛霉、安大略假单胞菌、真皮毛孢子菌、青霉、白地霉、灰绿曲霉、蜡叶散囊菌等；比对结果表明存在3株好干性酵母菌和5株阿姆斯特丹散囊菌，说明优势菌存在多种生态类型。采用DGGE指纹图谱能更系统、更真实地反映茯砖茶发花发酵过程中真菌群落结构和多样性动态变化。

关键词: 四川裂腹鱼, 耳石, 碳同位素, 氧同位素

Abstract: To explore fungal community structure and species composition in Fahua-fermentation process of Fuzhuan Brick Tea, the fungal variable region of 18S rDNA was amplified during different periods of Fahuafermentation process. Amplified products were separated by denaturing gradient gel electrophoresis (DGGE) and the bands were cloned, sequenced and aligned. The results show that two distinctive kinds of fungal communities, correlating to the fermentation periods, 0-8 d and 10-14 d respectively, were found. The dominant fungi of the former band include Wallemia muriae, Pichia kudriavzevii, Candida sp., and that of the latter include Eurotium amstelodami and W. muriae. The results of variable regions of 18S rDNA alignment showed that W. sebi, W. muriae, Debaryomyces hansenii, Saccharomyces cerevisiae, Candida sp., C. tropicalis, Lodderomyces sp., P. kudriavzevii, Rhodotorula graminis, Rhizomucor pusillus, Cryptococcus sp., E. amstelodami, Aspergillus penicillioides, R. miehei, C. ontarioensis, Trichosporon dermatis, Penicillium decumbens, Galactomyces geotrichum, A. glaucus, E. herbariorum were detected. The 18S rDNA alignment results also confirmed that there were 3 strains of W. sebi and 5 strains of E. amstelodami, displaying a wide variety of ecotypes within the dominant fungi. This study for the first time shows a detailed spectrum of fungi during the Fahuafermentation process. DGGE fingerprint is an important technique to analyze the comprehensive, detailed fungal community structure and diversity dynamics of Fuzhuan Brick Tea during this process.

Key words: carbon stable isotope, otolith, Schizothorax kozlovi, oxygen stable isotope

刘石泉1,2,赵运林1**,胡治远1. DGGE法初步解析茯砖茶发花过程中真菌群落结构[J]. 生态学杂志.

LIU Shi-quan1,2， ZHAO Yun-lin1**， HU Zhi-yuan1. Analysis of fungal community structure during the Fahua-fermentation process of Fuzhuan Brick Tea by DGGE technology.[J]. cje.

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