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一种赭球虫(Ochromonas sp.)的分离鉴定及食藻特性

杨晓景,彭玉辅,黄鑫,刘丽**,冯悦,夏雪山   

  1. (昆明理工大学生命科学与技术学院, 昆明 650500)
  • 出版日期:2015-04-10 发布日期:2015-04-10

Isolation, identification and grazing characteristics of a protozoan species Ocromonas sp.

YANG Xiao-jing, PENG Yu-fu, HUANG Xin, LIU Li**, FENG Yue, XIA Xue-shan   

  1. (Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China)
  • Online:2015-04-10 Published:2015-04-10

摘要:

从云南滇池分离到一株能吞食微藻的原生动物,根据形态特征及分子生物学鉴定为一种赭球虫(Ochromonas sp.)。本文研究了该原生动物的食藻范围及不同环境因素对其食藻效果的影响。结果表明: 该原生动物对惠氏微囊藻(Microcystis wesenbergii)具有很强的吞食能力,其与惠氏微囊藻(Microcystis wesenbergii)共培养至6 d时,微囊藻去除率达到90%以上;该赭球虫吞食微囊藻效果随其浓度增加而增强,在pH值为4~7、25~35 ℃条件下具有最佳的食藻效果,但高浓度苯酚对其生长有抑制和杀灭作用;该赭球虫易于培养,繁殖迅速,具有生物防控微囊藻水华的应用潜能。
 

关键词: GIS分析, 保护价值, 层次分析法, 熵权法, 国家湿地公园

Abstract:

A protozoan with the algophagous capability was isolated from the water samples of Dianchi Lake. Based on the morphological and genetic characteristics, it was identified as Ochromonas sp. The main factors influencing the grazing properties of Ochromonas sp. including algae species, protozoa concentration, culture temperature, time, pH and phenol concentration were investigated. The results showed that Ochromonas sp. had a strong grazing capability on Microcystis wesenbergii. When Ochromonas sp. was cocultured with M. wesenbergii for 6 days, the removal rate of M. wesenbergii was above 90%. Meanwhile, the grazing effect was enhanced with the increase of the initial concentration of the protozoa, and the best grazing capability was found when the culture pH and temperature were 4-7 and 25-35 ℃, respectively. However, high concentration of phenol could inhibit and kill the Ochromonas sp. The Ochromonas sp. was easy to culture and it reproduced rapidly. Therefore, it could be applied to control the water bloom of M. wesenbergii.
 

Key words: national wetland park, analytic hierarchy process (AHP), protection value, entropy method, GIS analysis