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• 研究报告 • 上一篇    下一篇

马缨丹对铅的生理响应及铅在亚细胞中的分布特征

罗弦1,方继宇2,张春梅2,贾永霞2*,张世熔3,徐小逊3,李婷2#br#   

  1. (1四川农业大学园艺学院, 成都 611130; 2四川农业大学资源学院, 成都 611130; 3四川农业大学环境学院, 成都 611130)
  • 出版日期:2016-09-10 发布日期:2016-09-10

Physiological response of Lantana camara L. under lead stress and subcellular distribution of lead.

LUO Xian1, FANG Ji-yu2, ZHANG Chun-mei2, JIA Yong-xia2*, ZHANG Shi-rong3, XU Xiao-xun3, LI Ting2#br#   

  1. (1College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China; 2College of Resources, Sichuan Agricultural University, Chengdu 611130, China; 3College of Environmental Sciences, Sichuan Agricultural University, Chengdu 611130, China).
  • Online:2016-09-10 Published:2016-09-10

摘要: 采用盆栽试验研究了不同浓度铅对马缨丹生长、抗氧化酶活性的影响,马缨丹对铅的吸收、转运,以及铅的亚细胞分布特征。结果表明:100 mg·kg-1铅处理时,马缨丹全株干重达到本次试验的最大值,且显著高于对照,铅处理浓度高于600 mg·kg-1时显著低于对照,植株生长受到抑制。铅处理浓度低于600 mg·kg-1时,马缨丹体内抗氧化酶活性较高,能够缓解铅处理诱导的氧化胁迫;高于600 mg·kg-1铅处理打破了马缨丹体内活性氧的产生与清除动态平衡,抗氧化酶活性降低,MDA含量和电解质渗漏率显著升高,引起严重的膜脂过氧化伤害。马缨丹体内铅含量随土壤浓度铅增加而逐渐增加,低于1200 mg·kg-1铅处理下,根系中铅含量高于地上部,高于此浓度时则相反,表明低于1200 mg·kg-1铅处理下,根系对铅有较强的固持能力,可限制铅向地上部的转运,减少对地上部的毒害;亚细胞分布研究表明,铅主要固定于根系和叶片的可溶性部分和细胞壁上,具有保护细胞器的分布特征。因此,马缨丹是一种铅耐性植物,其主要耐性机制是根系对铅的固持、可溶性部分和细胞壁对铅的区隔化以及抗氧化酶对活性氧物质的清除。

关键词: 中上层鲨鱼, 生态位, 东太平洋中部, 稳定同位素

Abstract: In this study, a pot experiment was conducted to investigate the growth, antioxidant enzyme activities and lead (Pb) uptake, translocation and Pb subcellular distribution of Lantana comara L. under Pb treatment with different concentrations. The results showed that the dry weight of L. camara reached the maximum when the Pb concentration was at 100 mg·kg-1, and significantly higher than the control, whereas the dry weight decreased significantly when soil Pb was higher than 600 mg·kg-1, and the growth of L. camara was inhibited significantly. When the Pb concentration in soil was lower than 600 mg·kg-1, the activity of antioxidant enzymes in L. camara was higher, which could alleviate the Pb induced oxidative stress. However, these activities displayed decreases when the soil Pb was higher than 600 mg·kg-1, MDA contents and electrolyte leakage in L. camara were enhanced significantly, and induced membrane lipid peroxidation damage. With the increasing of Pb concentration in soil, the contents of Pb in different organs of L. camara increased gradually, and Pb content in root was higher than that in shoot when the Pb concentration in soil was lower than 1200 mg·kg-1, and vise versa. It was suggested that L. camara root had a strong Pb retention capacity, which can reduce the toxicity of Pb to the organs of shoot. The proportion of Pb in soluble fraction in roots and leaves were highest, followed by cell wall. Therefore, L. camara is a Pbtolerant plant; its tolerance mechanism includes antioxidant system response, root retention, isolation in soluble fraction and cell wall immobilization.

Key words: pelagic shark, stable isotope, Central Eastern Pacific., niche