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“灭非灵”对尼罗罗非鱼鳃、肝和肾组织结构的影响

马光明1,2,顾党恩1,3,牟希东1,3,罗渡1,3,朱赟杰1,2,罗建仁1,3,胡隐昌1,3**   

  1. (1中国水产科学研究院珠江水产研究所, 农业部热带亚热带水产资源利用与养殖重点实验室, 广州 510380; 2上海海洋大学水产与生命学院, 上海 201306; 3中国水产科学研究院珠江水产研究所, 中国水产科学研究院珠江流域渔业资源养护与生态修复重点实验室, 广州 510380)
  • 出版日期:2015-04-10 发布日期:2015-04-10

Histopathology in gill, liver and kidney tissues of Nile tilapia (Oreochromis niloticus) exposed to “Mie fei ling”, a structurally specific drug.

MA Guang-ming1,2, GU Dang-en1,3, MU Xi-dong1,3, LUO du1,3, ZHU Yun-jie1,2, LUO Jian-ren1,3, HU Yin-chang1,3**   

  1. (1Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture, Guangzhou 510380, China; 2College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;
    3Key Laboratory of the Conservation and Ecological Restoration of Fishery Resource in Pearl River, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 510380, China)
  • Online:2015-04-10 Published:2015-04-10

摘要:

通过“灭非灵”对尼罗罗非鱼(Oreochromis niloticus) (体重为34.65±5.69 g)的急性毒性试验及对鳃、肝、肾的组织学研究,从组织学角度探讨了“灭非灵”对尼罗罗非鱼的致死机理。结果表明:“灭非灵”对尼罗罗非鱼的24、48、72和96 hLC50分别为0.148、0.103、0.048和0.032 mg·L-1;其组织病理学损伤表现为鳃小片萎缩、卷曲、坏死、脱落和融合,鳃间隙分泌大量的粘液细胞;肝细胞肿大,空泡化,细胞界限模糊,细胞核固缩;肾细胞肿大,充血;“灭非灵”对3种组织的损伤程度为鳃>肝脏>肾脏,3种组织的损伤很有可能是造成尼罗罗非鱼死亡的主要原因。
 

关键词: 土壤团聚体, 有机碳, 有机碳储量, 旱砂田

Abstract: An acute toxic experiment of “Mie fei ling” on Nile tilapia (body weight: 34.65±5.69 g) was conducted to study the histopathology changes in gill, liver and kidney tissues and the mechanism of specific poison effect on Nile tilapia was explained. Results showed that the 24, 48, 72 and 96 hLC50 values of “Mie fei ling” on Nile tilapia were 0.148, 0.103, 0.048 and 0.032 mg·L-1, respectively. Necrosis, desquamation, fusion, shortening and curling of secondary lamellae, and secretion of mucous cells were observed in gill tissues after exposed to “Mie fei ling”. Hepatic lesions in the fish exposed to “Mie fei ling” were characterized by hypertrophy of hepatocytes, vacuolar degeneration, nuclear pycnosis, congestion and cloudy degeneration of hepatocyte. Tubular degenerations and congestion were observed in the kidney tissues of fish after the exposure. The degree of tissue damage by “Mie fei ling” was in order of gill>liver>kidney. Therefore, the main cause of death of Nile tilapia exposed to “Mie fei ling” was likely due to its harm on tissues.

Key words: gravel-mulched field, soil organic carbon, soil organic carbon stock, soil aggregate