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褐藻胶寡糖对毒死蜱胁迫下小麦幼苗生理生化指标的影响

张守栋1,3,张同作1,韩晓弟2**,苏建平1   

  1. (1中国科学院西北高原生物研究所, 西宁 810001; 2山东大学(威海)海洋学院, 山东威海, 264209; 3中国科学院大学, 北京 100049)
  • 出版日期:2015-05-10 发布日期:2015-05-10

Alleviation effects of spraying alginate-derived oligosaccharide on physiological indexes of wheat seedlings under chloryrifos stress.

ZHANG Shou-dong1,3, ZHANG Tong-zuo1, HAN Xiao-di2**, SU Jian-ping1   

  1. (1Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China; 2Marine College, Shandong University at Weihai, Weihai 264209, Shandong, China; 3University of Chinese Academy of Sciences, Beijing 100049, China)
  • Online:2015-05-10 Published:2015-05-10

摘要:

褐藻胶寡糖对植物的生长发育具有调控作用。为了探讨褐藻胶寡糖对植物抗逆性的作用,以青丰Ⅰ号小麦种子为试验材料,探究了不同浓度褐藻胶寡糖(0、0.05%、0.1%、0.2%、0.4%)对毒死蜱胁迫下小麦生理特性的缓解作用。结果表明:毒死蜱显著降低小麦幼苗中叶绿素a、叶绿素b及总叶绿素含量(P<0.05),使脯氨酸、可溶性蛋白及可溶性糖含量显著升高(P<0.05),生物量降低,抑制地上部分生物量积累,根冠比增加;褐藻胶寡糖对这些生理生化指标的变化起到显著的缓解作用(P<0.05);浓度为0.4%的褐藻胶寡糖缓解作用最为显著(P<0.01)。因此,褐藻胶寡糖使毒死蜱对小麦幼苗的伤害有明显缓解作用,增强植物对毒死蜱胁迫的抗性。

 

关键词: 土壤有机碳密度, 土地利用, 克里格插值, 空间异质性, 水田和水浇地

Abstract: Alginatederived oligosaccharide plays an aregulatory role in the growth and development of plants. The alleviation effects of spraying alginatederived oligosaccharide at different concentrations (0, 0.05%, 0.1%, 0.2%, 0.4%) on physiological characters of wheat (Qingfeng No. 1) seedlings under chlorpyrifos stress were investigated in order to discuss the influences of alginatederived oligosaccharide on plant resistance. The results showed that chlorpyrifos decreased the contents of chlorophyll a, chlorophyll b and total chlorophyll in wheat seedlings (P<0.05), but significantly increased the contents of proline, soluble protein and soluble sugars (P<0.05), thus inhibited the accumulation of total biomass and aboveground biomass, making the ratio of root to shoot increase. By contrast, spraying alginatederived oligosaccharide played a significant role in mitigation in all the above mentioned physiological and biochemical changes (P<0.05), especially at the concentration of 0.4% (P<0.01). It was concluded that alginatederived oligosaccharide improved the physiological activity of wheat seedlings to some extent, thus strengthened the plant resistance against chlorpyrifos stress.

Key words: paddy field and irrigable land, soil organic carbon density, Kriging interpolation, spatial heterogeneity, land use type