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混合碱(NaHCO3和Na2CO3)胁迫对高粱幼苗渗透调节和离子平衡的影响

尚培培,李丰先,周宇飞,彭峥,高铭悦,韩熠,许文娟,黄瑞冬**   

  1. (沈阳农业大学农学院, 沈阳 110866)
  • 出版日期:2015-07-10 发布日期:2015-07-10

Effects of mixed alkaline (NaHCO3 and Na2CO3) stress on osmotic regulation and ion balance of sorghum seedlings.

SHANG Pei-pei, LI Feng-xian, ZHOU Yu-fei, PENG Zheng, GAO Ming-yue, HAN Yi, XU Wen-juan, HUANG Rui-dong**   

  1. (College of Agronomy, Shenyang Agricultural University, Shenyang 110866, China)
  • Online:2015-07-10 Published:2015-07-10

摘要: 以高粱耐碱品种(四杂25号)和碱敏感品种(龙杂9号)为研究材料,采用人工气候箱内营养液培养,研究不同浓度混合碱(NaHCO3和Na2CO3摩尔比为9∶1)胁迫对高粱幼苗叶片和根系渗透调节物质和离子平衡的影响。结果表明:随着混合碱浓度和pH值的增加,脯氨酸、可溶性蛋白、可溶性糖以及游离氨基酸等渗透调节物质含量在叶片和根系中均有显著增加;四杂25号叶片和根系中渗透调节物质含量的增幅大于龙杂9号,叶片中的渗透调节物质含量高于根系;随碱胁迫浓度的增加,两个高粱品种叶片和根系中Na+的浓度显著增加,而K+和Ca2+浓度显著减少,四杂25号K+和Ca2+浓度减小程度小于龙杂9号;叶片离子中以K+的含量最大,而根系中以Na+的含量最大,说明高粱幼苗叶片和根系所需平衡渗透势的阳离子种类和数量有所差异;根系受碱胁迫的破坏程度较叶大,其渗透调节和离子平衡能力弱于叶片,说明高粱根系对碱胁迫更加敏感。

关键词: 水文连通性, 植被格局, 汇流路径长度, 情景模拟, 河岸缓冲带

Abstract: Two sorghum cultivars, one alkaline tolerant (Siza 25) and one alkaline sensitive (Longza 9), were grown in nutrient solution in a controlled environment facility. The effects of different levels of mixed alkaline (NaHCO3∶Na2CO3=9∶1) stress on sorghum seedlings were assessed by measuring osmotic regulation substances and ion balance in the leaves and roots. The results showed that the concentrations of osmolytes, i.e. proline, soluble protein, soluble sugar and free amino acid, in both leaves and roots were significantly enhanced with the increase of mixed alkaline concentration and pH value. The concentrations of osmolytes increased in both leaves and roots, being higher in Siza 25 than in Longza 9 and higher in leaves than in roots. With the increase of mixed alkaline concentration, the concentration of Na+ in leaves and roots increased in both cultivars, but the concentrations of K+ and Ca2+ decreased. In addition, the reductions in K+ and Ca2+ concentrations were less in Siza 25 than in Longza 9. K+ concentration was the highest in leaves and Na+ concentration was the highest in roots, implying that there was a difference between the kinds and quantities of ions in balancing the osmotic potential in leaves and roots. Roots were more adversely affected by alkali stress and its ability of osmotic regulation and ion balance was weaker than leaves, suggesting that sorghum roots are more sensitive to alkali stress than its leaves.

Key words: hydrological connectivity, flow length, riparian buffer zone., vegetation pattern, scenario simulation