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氮、磷、铁对三角褐指藻诱变株生长、总脂及脂肪酸的影响

梁晶晶,蒋霞敏*,叶丽,韩庆喜   

  1. （宁波大学海洋学院， 浙江宁波 315211）
  • 出版日期:2016-01-10 发布日期:2016-01-10

Effects of nitrogen, phosphorus and iron on the growth, total lipid content and fatty acid composition of Phaeodactylum tricornutum mutant strain.

LIANG Jing-jing, JIANG Xia-min*, YE Li, HAN Qing-xi   

  1. (School of Marine Sciences, Ningbo University, Ningbo 315211, Zhejiang, China)
  • Online:2016-01-10 Published:2016-01-10

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摘要： 为了优化微藻培养条件，采用单因子试验研究了不同氮浓度（5、10、15、20、25、30和35 mg·L-1）、不同磷浓度（0.5、1.0、1.5、2.0、2.5、3.0和3.5 mg·L-1）、不同铁源（FeCl3、FeC6H5O7和FeSO4）和铁浓度（0、0.10、0.25、0.50和1.00 mg·L-1）对三角褐指藻紫外诱变株MP2的影响。结果表明：氮、磷、铁对MP-2的生长、总脂含量和脂肪酸组成影响显著（P<0.05）；MP-2生长最适氮浓度为20 mg·L-1，其生长速率K值和生物量分别为(0.384±0.004)和（0.25±0.01）g·L-1，氮浓度为30 mg·L-1时总脂积累量最高（26.51±1.96）%，氮浓度25 mg·L-1时PUFA积累最高［（37.78±0.35）%］；MP-2生长最适磷浓度为2.5 mg·L-1，生长速率K值和生物量分别为（0.305±0.010）和（0.28±0.02）g·L-1，磷浓度1.0 mg·L-1时总脂积累量最高\[（21.79±0.89）%\]，磷浓度为2.5 mg·L-1时PUFA积累最高\[（39.33±0.38）%\]；FeSO4为铁源时生长最佳，显著优于其余各组（P<0.05），生长最适铁浓度为0.50 mg·L-1，生长速率K值和生物量分别为（0.495±0.006)和（0.87±0.04）g·L-1，铁浓度0.25 mg·L-1时总脂积累量最高\[（28.86±0.50）%\]，铁浓度1.0 mg·L-1时PUFA积累最高\[（41.73±0.42）%\]。由此可见，MP-2生长、总脂和PUFA积累所需的氮、磷、铁需求量不同，在生产性培养时最好采用二次培养。

关键词: 蒸腾耗水量, 圆叶乌桕, 液流密度, 喀斯特, 菜豆树

Abstract: To optimize the cultivation conditions of microalgae, singlefactor test was performed to reveal the effects of different nutritional conditions on Phaeodactylum tricornutum mutant strain MP-2, including nitrogen (5, 10, 15, 20, 25, 30, 35 mg·L-1), phosphorus (0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5 mg·L-1), iron sources (FeCl3, FeC6H5O7, FeSO4) and their concentrations (0, 0.10, 0.25, 0.50, 1.00 mg·L-1). The results showed that all these factors exerted significant effects on the growth, total lipid content and fatty acid composition of MP-2 (P<0.05). The highest growth rate was obtained with the nitrogen concentration of 20 mg·L-1, with the growth rate K values and biomass of 0.384±0.004 and (0.25±0.01) g·L-1, respectively. The highest total lipid content (26.51±1.96) % was achieved when the nitrogen concentration was 30 mg·L-1, and the highest PUFA content (37.78±0.35) % occurred when it was 25 mg·L-1. MP-2 got the fastest growth and highest lipid content when the phosphorus concentration was 2.5 mg·L-1, and the growth rate K value and biomass were 0.305±0.010 and (0.28±0.02) g·L-1, respectively. The total lipid content achieved the highest value (21.79±0.89) % when the phosphorus concentration was 1.0 mg·L-1, and the highest PUFA content occurred when it was 2.5 mg·L-1. FeSO4 was proved to be the optimal iron source, and showed significant difference from other iron sources (P<0.05). The optimal FeSO4 concentration was 0.50 mg·L-1, with growth rate K value and biomass of （0.495±0.006） and (0.87±0.04) g·L-1, respectively. The highest total lipid content (28.86±0.50) % was achieved when the FeSO4 concentration was 0.25 mg·L-1, and the highest PUFA content occurred when it was 1.0 mg·L-1. The highest growth rate and total lipid and PUFA contents were obtained with different concentrations of nitrogen, phosphorus and iron, so a cultivation strategy should be adopted in mass culture.

Key words: Karst, sap flow density, Triadica rotundifolia, Radermachera sinica, transpiration water consumption.

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