五种鱼类标志对草鱼血液生化指标的影响

生态学杂志

五种鱼类标志对草鱼血液生化指标的影响

俞立雄^1,2,段辛斌²,陈大庆^2*,罗宏伟^1,2,刘绍平²,高雷^2#br#

（¹西南大学生命科学学院，重庆 400715； ^2农业部长江中上游渔业资源环境科学观测实验站，中国水产科学研究院长江水产研究所，武汉 430223）

出版日期:2017-04-10 发布日期:2017-04-10

Effects of five fishing taggers on biochemical parameters of Ctenopharyngodon idellus.

YU Li-xiong^1,2, DUAN Xin-bin², CHEN Da-qing^2*, LUO Hong-wei^1,2, LIU Shao-ping², GAO Lei^2#br#

(¹ Faculty of Life Sciences, Southwest University, Chongqing 400715, China; ² Key Field Scientific Observing and Experimental Station of Fishery Resources and Environment of the Middle and Upper Reaches of the Yangtze River of the Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Wuhan 430223, China).

Online:2017-04-10 Published:2017-04-10

摘要/Abstract

摘要： 实验旨在研究鱼类标志对草鱼（Ctenopharyngodon idellus）血液生化指标的影响。选用108尾体长（18.54±2.04 cm）、体重（109.04±36.36 g）的草鱼，设6个处理组，分别为超声波标志组、Passive Integrated Transponder（PIT）标志组、荧光标志组、切鳍标志组、T型标志组和对照组。每个处理组设置3个重复，每个重复6尾草鱼，并在标志后的1、7、15 d从每个重复组随机选2尾草鱼进行抽血检验。结果表明：超声波标志对草鱼血液生化指标的影响最大，标志后1 d草鱼血清球蛋白、谷丙转氨酶、谷草转氨酶浓度显著性升高，氯离子浓度在标志后7及15 d也维持在较高水平，标志后15 d球蛋白浓度降低，但仍然显著性高于对照组；PIT标志、切鳍标志及T型标志对草鱼血液生化指标影响相对较小，PIT标志组草鱼在标志后7 d氯离子浓度显著性升高，15 d后恢复正常；切鳍标志组草鱼在标志后1 d甘油三酯浓度显著性下降，7 d后恢复正常；T型标志组草鱼在标志后1 d谷草转氨酶浓度显著性升高，7 d后恢复正常；荧光标志对草鱼血液生化指标影响最小，标志后所有生化指标与对照组均无显著性差异。荧光标志适用于大规模标志，标志后即可放流；PIT标志、切鳍标志和T型标志对草鱼的生理状况都产生一定的影响，建议在标志后至少暂养7 d再放流；超声波标志后草鱼至少暂养15 d，待其伤口愈合，消除标志对其产生的负面影响后再放流。

关键词: 山地果园, 黄绵土, 经验公式, 集雨壤中防渗

Abstract: This study aims to assess the effects of five tagging methods on the biochemical para-meters of grass carps (Ctenopharyngodon idellus). The tagging methods include ultrasound mar-king, passive integrated transponder (PIT) marking, fluorescent marking, fins amputated marking, T-type marking, and control. One hundred and eight grass carps, with body length (18.54±2.04) cm and body weight (109.04±36.36) g, were randomly allocated into 6 groups, and each group contained three replications. Two blood samples were extracted randomly from each replication 1, 7 and 15 days after tagging. The results showed that levels of globulin (GLB), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in ultrasound marking group increased significantly 1 day after tagging. After 7 days, the level of chlorine (Cl) was elevated and GLB remained at high level, whereas the concentrations of ALT and AST declined to the normal level. After 15 days, the level of GLB declined but still remained higher than that in the control group. There was a significant increase in concentration of Cl in PIT marking group 7 days after tagging, and it declined to the normal level at day 15. The level of triglyceride (TG) in fins amputated marking group decreased significantly while the concentration of AST in T-type marking group increased significantly 1 day after tagging and both of them recovered at day 7. All of the biochemical parameters of fluorescent marking showed no significant difference as compared with the control group. In conclusion, fluorescent marking is appropriate for largescale tagging and the fish can be released soon after tagging. Grass carps with PIT marking, fins amputated marking or T-type marking are recommended to be kept for 7 days to eliminate the bad effects before release. Ultrasound marking has the greatest effect on the health status of grass carp, and it is advisable for fish to be kept at least for 15 days before release.

Key words: hilly apple orchard, rainwater harvesting-soil anti-seepage, empirical equation, loessial soil

俞立雄1,2,段辛斌2,陈大庆2*,罗宏伟1,2,刘绍平2,高雷2. 五种鱼类标志对草鱼血液生化指标的影响[J]. 生态学杂志.

YU Li-xiong1,2, DUAN Xin-bin2, CHEN Da-qing2*, LUO Hong-wei1,2, LIU Shao-ping2, GAO Lei2. Effects of five fishing taggers on biochemical parameters of Ctenopharyngodon idellus.[J]. cje.

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