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基于Illumina MiSeq技术分析不同地域加工的茯砖茶中微生物群落多样性

赵仁亮1,2,3,胥伟1,2,吴丹1,2,姜依何1,2,朱旗1,2*   

  1. (1湖南农业大学茶学教育部重点实验室, 长沙 410128; 2国家植物功能成分利用工程技术研究中心, 长沙 410128;3河南农业大学园艺学院, 郑州 450002)
  • 出版日期:2017-07-10 发布日期:2017-07-10

Microbial community diversity of Fu brick tea produced in different regions by Illumina MiSeq technology.

ZHAO Ren-liang1,2,3, XU Wei1,2, WU Dan1,2, JIANG Yi-he1,2, ZHU Qi1,2*#br#   

  1. (1Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University Changsha 410128, China; 2National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients,  Changsha 410128, China; 3College of Horticulture, Henan Agricultural University, Zhengzhou 450002, China).
  • Online:2017-07-10 Published:2017-07-10

摘要: 为研究不同地域加工的茯砖茶中发花微生物群落结构及多样性,本文采用Illumina Miseq技术对其微生物群落结构进行了解析。结果表明:不同地域加工的茯砖茶中细菌种类丰富,分为9个门、16个纲、29个目、50个科、66个属,其中厚壁菌门中的乳球菌属(Lactococcus)占绝对优势;湖南产区的茯砖茶中细菌丰度和多样性最高。真菌分为2个门、6个纲、6个目、7个科、7个属,其中曲霉属(Aspergillus)是绝对优势菌种,在每个样品中的丰度均在92%以上;此外,还检测到了丰度低于1%的酵母属,分别是假丝酵母属、耐碱酵母属和毕赤酵母属,且在不同茶样中的丰度存在差异;陕西产区的茯砖茶中真核微生物菌群丰度最高。聚类分析发现,同一产区的茯砖茶样品距离较近,表明地域环境及加工工艺是影响茯砖茶发花中微生物群落结构的主要因素。

关键词: 净初级生产力, Hurst指数, MOD17A3, 秦巴山区

Abstract: Illumina Miseq technology was used to study the microbial community structure and diversity of Fu brick tea made in different regions. The results showed that the bacterial species of Fu brick tea were various, which consisted of 9 phyla, 16 classes, 29 orders, 50 families, 66 genera, and Lactococcus was dominant fungi. The prokaryotic microbial abundance and diversity of Fu brick tea made in Hunan were highest. Fungi analysis results showed that there were 2 phyla, 6 classes, 6 orders, 7 families and 7 genera, and the Aspergillus was the absolutely dominant strain, with the abundance of over 92% in each sample. In addition, the abundance of yeasts was less than 1%, and the yeasts included Candida, Pichia, Galactomyces, with different abundances for the samples produced in different regions. The eukaryotic microbial abundance of Fu brick tea made in Shaanxi was the highest. The cluster analysis showed that the samples of Fu brick tea produced in the same area were closer, suggesting that the regional environment and processing technology were the main factors affecting microbial community structure of Fu brick tea.

Key words: Hurst index, Qinling-Daba Mountains, net primary productivity, MOD17A3