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低养分基质下沙地柏和樟子松苗木对遮荫的响应

孟鹏*   

  1. (辽宁省固沙造林研究所, 阜新 123000)
  • 出版日期:2017-11-10 发布日期:2017-11-10

Responses of Juniperus sabina and Pinus sylvestris var. mongolica seedlings to shading in low nutrient conditions.

MENG Peng*   

  1. (Liaoning Province SandFixation and Afforestation Research Institute, Fuxin 123000, Liaoning, China).
  • Online:2017-11-10 Published:2017-11-10

摘要: 为了研究遮荫对贫瘠土壤上生长的植物存活率、相对生长率(RGR)、生物量分配模式及光合特性的影响,本文以耐荫的沙地柏和不耐荫的樟子松苗木为试材,进行全光和不同遮荫处理(35%、65%和95%),遮荫处理时间为2个生长季。结果表明,随着光照强度减弱,沙地柏的存活率和生长量没有明显变化,总生物量和RGR均呈先上升后下降的趋势,且RGR指标均为正值,茎根比显著提高;而樟子松存活率和生长量显著降低,总生物量和RGR一直呈下降趋势,且95%遮荫率下RGR为负值,茎根比显著降低。沙地柏的最大光化学效率(Fv/Fm)对遮荫更加敏感,在35%遮荫率下就显著增加,而樟子松在65%遮荫率时才开始显著增加。JIP测定进一步表明,遮荫下沙地柏标准化荧光值(Vt)从O相到接近P相的过程中均明显低于全光照(CK),电子越过QA的能量占反应中心捕获能量的比例(ψo)、用于电子传递的量子比率(ψEo)均提高,且质体醌库(Sm)变大,质体醌库还原速率(Sm/Tfm)提高、初级醌受体被还原的次数(N)增多,单位激发态面积用于电子传递的能量(ETo/CSo)增加、反应中心数目(RC/CSo)增多,单位反应中心电子传递的能量(ETo/RC)增加,同时单位激发态面积(DIo/CSo)和单位反应中心(DIo/RC)的热耗散的能量均降低,最终导致其性能指数(PIABS, PICSo, PICSm)明显高于全光照。遮荫下樟子松标准化荧光值从O相到接近P相的过程中均明显高于CK,除热耗散指标外以上这些荧光参数与CK没有明显差异,甚至更低,而DIo/RC指标保持不变,最终导致其性能指数(PIABS, PICSo, PICSm)与CK没有明显差异。以上结果暗示,沙地柏在较低的光强下能维持较高的生长量,通过维持正向碳平衡保持了较高的存活率,增加了地上生物量的比例,提高了光反应阶段电子传递的能量水平;而樟子松碳收支失衡导致了较低的存活率,地上生物量比例下降,电子传递的能量水平降低。

Abstract: To determine the effects of shade on survival rate, relative growth rate (RGR), biomass allocation and photosynthesis characteristics, potted seedlings of shadetolerant Juniperus sabina and shadeintolerant Pinus sylvestris var. mongolica in lownutrient substrate were grown without shade or under a 35%, 65% or 95% reduction of full sunlight over two growing seasons. The results showed that, with decreased light availability, J. sabina growth and survival did not change significantly. Total biomass and RGR increased and then decreased; RGR indicators were positive; and the shoot/root ratio increased significantly. In contrast, the survival rate and growth of P. sylvestris var. mongolica decreased significantly. Total biomass and RGR decreased; the RGR was negative under 95% shade; and the shoot/root ratio decreased significantly. The maximum photochemical efficiency (Fv/Fm) of J. sabina was more sensitive to shade and increased significantly under 35% shade, while the Fv/Fm of P. sylvestris var. mongolica increased significantly until 65% shade. A JIPtest showed that the standardized fluorescence values (Vt) of J. sabina under shade were lower than those under the nonshading treatment (CK) from initial O phase to final P phase. Furthermore, the proportion of electron energy across the QA reaction center to the whole energy captured (ψo), quantum yield of electron transfer (ψEo), size of plastoquinone pool (Sm), plastoquinone pool reduction rate (Sm/Tfm), number of reduced primary quinone acceptors (N), and energy for electron transfer per excited crosssection (ETo/CSo) all increased. The density of reaction centers (RC/CSo) increased, while dissipation per excited crosssection (DIo/CSo) and per reaction center (DIo/RC) decreased, leading to significantly higher performance indexes (PIABS, PICSo, PICSm) under shade than under CK. The Vt of P. sylvestris var. mongolica under shade was higher than under CK from O phase to P phase; however, the above fluorescence parameters were lower or were not significantly different under shade except for the heat dissipation indexes. DIo/RC remained unchanged in P. sylvestris var. mongolica under shade, and the performance indexes (PIABS, PICSo and PICSm) also remained unchanged. The above results indicated that J. sabina was able to maintain high growth and survival under low light intensity by maintaining a positive carbon balance and increasing the proportion of aboveground biomass and electron transfer energy levels during the lightreaction stage. In contrast, P. sylvestris var. mongolica had a low survival rate due to a carbon budget imbalance, and the decreases in proportion of aboveground biomass and electron transfer energy level under shading conditions.