有机肥和解磷固氮菌配施对缺碳黄棕壤养分特性的协同效应

doi:10.13287/j.1001-9332.202010.023

应用生态学报 ›› 2020, Vol. 31 ›› Issue (10): 3413-3423.doi: 10.13287/j.1001-9332.202010.023

有机肥和解磷固氮菌配施对缺碳黄棕壤养分特性的协同效应

王志康¹, 徐子恒¹, 陈紫云², 洑香香^1*

¹南京林业大学林学院, 南方现代林业协同创新中心, 南京 210037;
²中国科学院南京土壤研究所, 南京 210008

收稿日期:2020-04-17 接受日期:2020-08-02 出版日期:2020-10-15 发布日期:2021-04-15
通讯作者: * E-mail: xxfu@njfu.edu.cn
作者简介:王志康, 男, 1993年生, 博士研究生。主要从事人工林定向培育和土壤微生态研究。E-mail: wzk@njfu.edu.cn
基金资助:
江苏省重点研发计划(现代农业)重点项目(BE2019388)和江苏高校优势学科建设工程项目(PAPD)资助

Synergistic effects of organic fertilizer coupled with phosphate-solubilizing and nitrogen-fixing bacteria on nutrient characteristics of yellow-brown soil under carbon deficiency

WANG Zhi-kang¹, XU Zi-heng¹, CHEN Zi-yun², FU Xiang-xiang^1*

¹Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China;
²Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China

Received:2020-04-17 Accepted:2020-08-02 Online:2020-10-15 Published:2021-04-15
Contact: * E-mail: xxfu@njfu.edu.cn
Supported by:
Key Research and Development Program of Jiangsu Province (BE2019388) and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

摘要/Abstract

摘要： 研究不同有机肥配比下解磷固氮细菌对土壤养分和相关酶活性的动态影响,可为筛选合适的菌肥类型、有机肥配比和施肥周期提供参考。试验设置0%、4%、8%、12%(质量比)4种有机肥配比,选择巨大芽孢杆菌、荧光假单胞菌2种解磷菌和褐球固氮菌、巴西固氮螺菌2种固氮菌,以亚热带贫瘠黄棕壤为研究对象,在控制条件下(28 ℃、避光)持续土壤培养60 d,以探讨不同有机肥水平和不同取样时间(培养第3、8、16、30、45、60天)4种细菌单独或混合接种对土壤有效态养分和相关酶活性的影响。结果表明: 随着有机肥含量的增加,菌肥处理土壤的有效氮和有效磷含量逐渐提高,表现为12%>8%>4%>0%。随着培养时间的延长,各处理土壤养分基本呈现先增加后降低的趋势。相对于单施有机肥,配施有机肥和菌肥能够显著提高和延长菌肥对土壤养分含量和酶活性的增益。菌肥对土壤养分特性的影响在不同有机肥配比下呈现不同的规律: 有机肥含量较低(0%～4%)时,虽然菌肥显著提高了土壤有效养分含量,但在初期各菌肥间差异并不显著;随着有机肥含量的提高和培养时间的延长,解磷菌(尤其是巨大芽孢杆菌)显著提高了有效磷含量,固氮菌(尤其是巴西固氮螺菌)显著提高了有效氮含量。4种细菌混合菌肥主要呈现解磷功能,比单施解磷菌具有更明显的优势,而固氮功能未得到显著提高。相关分析表明,土壤养分含量和酶活性大小呈显著正相关关系,而酶活性受土壤培养时间和碳氮比的影响。因此,针对该土壤类型,菌肥能够在短期内显著提高土壤养分含量,但菌肥特定功能的发挥依赖于有机碳的输入和土壤碳氮比的大小,配施合适比例的有机肥(8%～12%)能够提高并延长菌肥的改良效果;每45～60 d需进行菌肥复施,以保证其存活率和持续效果。

关键词: 解磷菌, 固氮菌, 有机肥, 土壤改良

Abstract: Understanding the dynamics of phosphate-solubilizing and N₂-fixing bacteria on soil nutrient and related enzyme activity under different organic fertilizer proportions (OFP) could provide references for screening appropriate inoculant type, OFP, and fertilization period. Here, we set four OFP levels (mass ratio: 0%, 4%, 8%, 12%) and inoculated two phosphate-solubilizing bacteria (Bacillus megaterium, Pseudomonas fluorescens) and two N₂-fixing bacteria (Azotobacter chroococcum, Azospirillum brasilence) in the subtropical yellow-brown barren soil. After a 60-day soil incubation under controlled conditions (28 ℃, darkness), we examined the impacts of single/mixed applications of beneficial bacteria on soil available nutrients and related enzyme activities at different OFP levels and different sampling times (3rd, 8th, 16th, 30th, 45th, 60th day). The results showed that soil available nutrient contents increased with the elevated OFP levels, and exhibited as 12%>8%>4%>0%. With the extension of culture time, soil nutrient contents in all treatments first increased and then decreased. Compared with the single application of organic fertilizer, combined application of organic fertilizer and bacterial inoculants resulted in higher and longer improvement of soil nutrient contents and enzyme activities. The effects of inoculants on soil nutrient properties varied across four OFP levels. When the OFP was low (0-4%), inoculation significantly increased soil available nutrient contents, with no the differences between inoculants at the initial stage. However, with the extension of the culture time and the elevation of OFP, phosphate-solubilizing bacteria (especially for B. megaterium) significantly increased available phosphorus content while N₂-fixing bacteria (especially for A. brasilence) significantly increased available nitrogen content. The mixed inoculant with four strains showed phosphate-solubilizing effect on soil and performed better than the single application of phosphate-solubilizing bacteria, but without prominent effect on nitrogen fixation. Soil nutrient contents were positively correlated with enzyme activity, which was affected by both cultural time and carbon-nitrogen ratio. Bacterial inoculations could significantly increase nutrient contents in the short term, but the specific functions of beneficial bacteria on soil were highly dependent on organic carbon input and carbon-nitrogen ratio. Coupled application of inoculants and organic fertilizer at an appropriate OFP level (8%-12%) could increase and extend the soil-remediating effects, while the inoculation should be conducted with an interval of 45-60 days to ensure the survival rate and the consecutive effect on soil.

Key words: phosphate-solubilizing bacteria, nitrogen-fixing bacteria, organic fertilizer, soil amendment

王志康, 徐子恒, 陈紫云, 洑香香. 有机肥和解磷固氮菌配施对缺碳黄棕壤养分特性的协同效应[J]. 应用生态学报, 2020, 31(10): 3413-3423.

WANG Zhi-kang, XU Zi-heng, CHEN Zi-yun, FU Xiang-xiang. Synergistic effects of organic fertilizer coupled with phosphate-solubilizing and nitrogen-fixing bacteria on nutrient characteristics of yellow-brown soil under carbon deficiency[J]. Chinese Journal of Applied Ecology, 2020, 31(10): 3413-3423.

参考文献

[1] Vitousek PM, Porder S, Houlton BZ, et al. Terrestrial phosphorus limitation: Mechanisms, implications, and nitrogen-phosphorus interactions. Ecological Applications, 2010, 20: 5-15
[2] Wright IJ, Reich PB, Westoby M, et al. The worldwide leaf economics spectrum. Nature, 2004, 428: 821-827
[3] 李蓉, 周德明, 吴毅, 等. 杉木根际溶磷菌筛选及其部分特性的初步研究. 中南林业科技大学学报, 2012, 32(4): 95-99 [Li R, Zhou D-M, Wu Y, et al. Selection and characteristics of phosphate-solubilizing bacteria in rhizosphere of Cunninghaimia lanceolata. Journal of Central South University of Forestry & Technology, 2012, 32(4): 95-99]
[4] 王光华, 赵英, 周德瑞, 等. 解磷菌的研究现状与展望. 生态环境学报, 2003, 12(1): 96-101 [Wang G-H, Zhao Y, Zhou D-R, et al. Review of phosphate-solubilizing microorganisms. Ecology and Environment, 2003, 12(1): 96-101]
[5] Bhardwaj D, Ansari MW, Sahoo RK, et al. Biofertilizers function as key player in sustainable agriculture by improving soil fertility, plant tolerance and crop productivity. Microbial Cell Factories, 2014, 13: 66
[6] 李明哲. 农田化肥施用污染现状与对策. 河北农业科学, 2009, 13(5): 65-67 [Li M-Z. Status and countermeasures of farmland pollution by chemical fertilizer application. Journal of Hebei Agricultural Sciences, 2009, 13(5): 65-67]
[7] 肖军, 秦志伟, 赵景波. 农田土壤化肥污染及对策. 环境保护科学, 2005, 31(5): 32-34 [Xiao J, Qin Z-W, Zhao J-B. Status and countermeasures of farmland soil polluted by chemical fertilizer. Environmental Protection Science, 2005, 31(5): 32-34]
[8] Valetti L, Iriarte L, Fabra A. Growth promotion of rapeseed (Brassica napus) associated with the inoculation of phosphate solubilizing bacteria. Applied Soil Ecology, 2018, 132: 1-10
[9] Yu X, Liu X, Zhu TH, et al. Co-inoculation with phosphate-solubilzing and nitrogen-fixing bacteria on solubilization of rock phosphate and their effect on growth promotion and nutrient uptake by walnut. European Journal of Soil Biology, 2012, 50: 112-117
[10] 王旭辉, 丁亚欣, 谈俊. 生物菌肥促生机制研究. 现代农业科技, 2010(4): 308-309 [Wang X-H, Ding Y-X, Tan J. Growth-promoting mechanism of bio-ferti-lizer. Modern Agricultural Sciences and Technology, 2010(4): 308-309]
[11] Yu X, Liu X, Zhu TH, et al. Isolation and characterization of phosphate-solubilizing bacteria from walnut and their effect on growth and phosphorus mobilization. Bio-logy and Fertility of Soils, 2011, 47: 437-446
[12] Bahrani A, Pourreza J, Joo MH. Response of winter wheat to co-inoculation with Azotobacter and arbuscular mycorrhizal fungi (AMF) under different sources of nitrogen fertilizer. American-Eurasian Journal of Agricultural & Environmental Sciences, 2010, 8: 95-103
[13] Askary M, Mostajeran A, Amooaghaei R, et al. Influence of the co-inoculation Azospirillum brasilense and Rhizobium meliloti plus 2,4-D on grain yield and N, P, K content of Triticum aestivum (Cv. Baccros and Mah-davi). American-Eurasian Journal of Agricultural & Environmental Sciences, 2009, 5: 296-307
[14] Hu J, Wei Z, Weidner S, et al. Probiotic Pseudomonas communities enhance plant growth and nutrient assimilation via diversity-mediated ecosystem functioning. Soil Biology and Biochemistry, 2017, 113: 122-129
[15] 库永丽, 徐国益, 赵骅, 等. 微生物肥料对猕猴桃高龄果园土壤改良和果实品质的影响. 应用生态学报, 2018, 29(8): 2532-2540 [Ku Y-L, Xu G-Y, Zhao Y, et al. Effects of microbial fertilizer on soil improvement and fruit quality of kiwifruit in old orchard. Chinese Journal of Applied Ecology, 2018, 29(8): 2532-2540]
[16] Gunasekara AS, Xing BS. Sorption and desorption of naphthalene by soil organic matter: Importance of aromatic and aliphatic components. Journal of Environmental Quality, 2003, 32: 240-246
[17] 齐连芬, 王丹丹, 牛瑞生, 等. 基于主成分分析的温室番茄最佳有机肥与微生物菌剂配比. 北方园艺, 2019(1): 13-19 [Qi L-F, Wang D-D, Niu R-S, et al. Comparison of optimum tomato ratio of organic fertilizer and microbial bacteria based on principal component analysis in solar greenhouse. Northern Horticulture, 2019(1): 13-19]
[18] Wang ZK, Chen ZY, Fu XX. Integrated effects of co-inoculation with phosphate-solubilizing bacteria and N₂-fixing bacteria on microbial population and soil amendment under C deficiency. International Journal of Environmental Research and Public Health, 2019, 16: 2442
[19] 罗贤安, 凃安千. 生物固氮研究中乙炔还原法的应用. 微生物学通报, 1979, 6(2): 37-40 [Luo X-A, Tu A-Q. Application of acetylene reduction in the study of biological nitrogen fixation. Microbiology China, 1979, 6(2): 37-40]
[20] Rashid MI, Mujawar LH, Shahzad T, et al. Bacteria and fungi can contribute to nutrients bioavailability and aggregate formation in degraded soils. Microbiological Research, 2016, 183: 26-41
[21] 朱浩, 刘珂欣, 刘维维, 等. 极端耐盐碱菌株的筛选及其菌肥对盐碱条件下小麦生长和土壤环境的影响. 应用生态学报, 2019, 30(7): 2338-2344 [Zhu H, Liu K-X, Liu W-W, et al. Screening of extreme salt-alkali tolerant strain and effect of its fertilizer on wheat growth and soil environment under saline-alkali condition. Chinese Journal of Applied Ecology, 2019, 30(7): 2338-2344]
[22] Yilmaz E, Sonmez M. The role of organic/bio-fertilizer amendment on aggregate stability and organic carbon content in different aggregate scales. Soil and Tillage Research, 2017, 168: 118-124
[23] 王若男, 洪坚平. 4种生物菌肥对盆栽油菜产量品质及土壤养分含量的影响. 山西农业大学学报: 自然科学版, 2016, 36(11): 774-778 [Wang R-N, Hong J-P. The influence of the four types of biological bacte-rial manure on the production quality and soil nutrient of the potted cole. Journal of Shanxi Agricultural Univer-sity: Natural Science, 2016, 36(11): 774-778]
[24] Wang ZK, Chen ZY, Xu ZH, et al. Effects of phosphate-solubilizing bacteria and N₂-fixing bacteria on nutrient uptake, plant growth, and bioactive compound accumulation in Cyclocarya paliurus (Batal.) Iljinskaja. Forests, 2019, 10: 772
[25] Huang YM, Wu QS, Yan L. Arbuscular mycorrhizal fungi alter plant growth, soil aggregate stability, and rhizospheric organic carbon pools of citrus. Advanced Materials Research, 2013, 610: 3063-3066
[26] 王利利, 董民, 张璐, 等. 不同碳氮比有机肥对有机农业土壤微生物生物量的影响. 中国生态农业学报, 2013, 21(9): 1073-1077 [Wang L-L, Dong M, Zhang L, et al. Effects of organic manures with different carbon-to-nitrogen ratios on soil microbial biomass of organic agriculture. Chinese Journal of Eco-Agriculture, 2013, 21(9): 1073-1077]
[27] 朱培淼, 杨兴明, 徐阳春, 等. 高效解磷细菌的筛选及其对玉米苗期生长的促进作用. 应用生态学报, 2007, 18(1): 107-112 [Zhu P-M, Yang X-M, Xu Y-C, et al. High effective phosphate-solubilizing bacteria: Their isolation and promoting effect on corn seedling growth. Chinese Journal of Applied Ecology, 2007, 18(1): 107-112]
[28] 毛晓洁, 王新民, 赵英, 等. 多功能固氮菌筛选及其在土壤生态修复中的应用. 生物技术通报, 2017, 33(10): 148-155 [Mao X-J, Wang X-M, Zhao Y, et al. Screening of multi-functional nitrogen fixing bacteria and their application in soil ecological restoration. Biotechnology Bulletin, 2017, 33(10): 148-155]
[29] 刘歌畅, 王留成, 姚旭, 等. 高效固氮菌Ecl分子鉴定及其在铁尾矿基质下接种效果研究. 江苏农业科学, 2019, 47(8): 279-284 [Liu G-C, Wang L-C, Yao X, et al. Molecular identification of high efficiency nitrogen-fixing bacteria Ecl and its inoculation effect in iron tailings matrix. Jiangsu Agricultural Sciences, 2019, 47(8): 279-284]

有机肥和解磷固氮菌配施对缺碳黄棕壤养分特性的协同效应

Synergistic effects of organic fertilizer coupled with phosphate-solubilizing and nitrogen-fixing bacteria on nutrient characteristics of yellow-brown soil under carbon deficiency

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	李廷强, 郝点. 我国耕地“非粮化”现状及其复耕培肥技术研究进展 [J]. 应用生态学报, 2023, 34(6): 1703-1712.
[2]	陆欣春, 范欣欣, 邹文秀, 严君, 陈旭, 韩晓增, 邓维娜. 肥沃耕层构建对白浆土土壤肥力和玉米产量的影响 [J]. 应用生态学报, 2023, 34(4): 883-891.
[3]	江尚焘, 栗晗, 彭海英, 梅新兰, 陈廷速, 徐阳春, 董彩霞, 沈其荣. 有机肥替代部分化肥对芒果丛枝菌根真菌群落的影响 [J]. 应用生态学报, 2023, 34(2): 481-490.
[4]	邓亚琴, 徐智, 张勇, 王宇蕴. 土壤微生物生物量氮对不同腐熟度有机肥的响应及对土壤矿质氮的调控作用 [J]. 应用生态学报, 2023, 34(1): 137-144.
[5]	孙健, 王亚艺, 张鑫鹏, 李松龄. 青海地区解磷微生物的筛选及对小油菜生长的影响 [J]. 应用生态学报, 2023, 34(1): 221-228.
[6]	安祥瑞, 江尚焘, 谢昶琰, 徐阳春, 董彩霞, 沈其荣. 减施化肥配施有机肥对荔枝园土壤微生物区系的影响 [J]. 应用生态学报, 2022, 33(4): 1099-1108.
[7]	李佳乐, 梁泳怡, 刘文杰, 杨秋, 徐文娴, 汤水荣, 王晶晶. 有机肥替代化学氮肥对橡胶幼苗生长和土壤环境的影响 [J]. 应用生态学报, 2022, 33(2): 431-438.
[8]	马龙, 王书停, 史雷, 张然, 王楷, 郑伟, 李紫燕, 翟丙年. 有机肥配施氮肥对西北旱地冬小麦产量、品质及土壤生物学特性的影响 [J]. 应用生态学报, 2022, 33(10): 2718-2724.
[9]	李圆宾, 李鹏, 王舒华, 徐璐瑶, 邓建军, 焦加国. 稻麦轮作体系下有机肥施用对作物产量和土壤性质影响的整合分析 [J]. 应用生态学报, 2021, 32(9): 3231-3239.
[10]	徐扬, 刘引, 彭政, 郭兰萍, 刘大会. 化肥减量配施有机肥对药用菊花产量、品质和药理活性的影响 [J]. 应用生态学报, 2021, 32(8): 2800-2808.
[11]	张黛静, 胡晓, 马建辉, 郭雨欣, 宗洁静, 杨雪倩. 耕作和培肥对豫中区小麦-玉米轮作系统土壤氮平衡和温室气体排放的影响 [J]. 应用生态学报, 2021, 32(5): 1753-1760.
[12]	佘晨兴, 林洪, 苏玉萍, 张勇, 林婉珍, 兰瑞芳. 闽江口-平潭海域有机解磷菌多样性及群落特征 [J]. 应用生态学报, 2021, 32(5): 1863-1872.
[13]	张路, 王杰, 王向涛, 廖李容, 宛倩, 刘国彬, 张超. 不同恢复方式对退化高寒草甸土壤nifH和chiA微生物群落结构的影响 [J]. 应用生态学报, 2021, 32(12): 4349-4358.
[14]	李然, 段英华, 孙楠, 邬磊, 张强, 靳东升, 郜春花, 洪坚平, 徐明岗. 煤矿区复垦土壤中有机肥的分解动态及其驱动因素 [J]. 应用生态学报, 2021, 32(12): 4467-4474.
[15]	卜容燕, 李敏, 韩上, 程文龙, 王慧, 孙志祥, 唐杉, 武际. 有机无机肥配施对双季稻轮作系统产量、温室气体排放和土壤养分的综合效应 [J]. 应用生态学报, 2021, 32(1): 145-153.