大兴安岭北部不同海拔天然林土壤胞外酶化学计量特征及其季节动态

doi:10.13287/j.1001-9332.202008.005

应用生态学报 ›› 2020, Vol. 31 ›› Issue (8): 2491-2499.doi: 10.13287/j.1001-9332.202008.005

大兴安岭北部不同海拔天然林土壤胞外酶化学计量特征及其季节动态

及利^1,2, 马立新³, 程政磊⁴, 祝清超³, 张岩¹, 杨雨春², 杨立学^1*

¹东北林业大学林学院, 森林生态系统可持续经营教育部重点实验室, 哈尔滨 150040;
²吉林省林业科学研究院, 长春 130033;
³内蒙古阿龙山林业局营林科, 内蒙古呼伦贝尔 022362;
⁴内蒙古阿龙山林业局病虫害防疫检疫站, 内蒙古呼伦贝尔 022362

收稿日期:2020-03-01 修回日期:2020-06-02 出版日期:2020-08-15 发布日期:2021-02-15
通讯作者: * E-mail: ylx_0813@163.com
作者简介:及利, 男, 1991年生, 博士研究生。主要从事森林培育和土壤生态学研究。E-mail: jlnefu@hotmail.com
基金资助:
中央高校基础科研业务费专项资金(2572019AA07,2572019CP16)、国家重点研发计划项目(2017YFD0601204)和黑龙江头雁创新团队计划项目(森林资源高效培育技术研发团队)资助

Stoichiometry of soil extracellular enzymes and its seasonal variation in natural forests with different altitudes in northern Greater Khingan Mountains, China

JI Li^1,2, MA Li-xin³, CHENG Zheng-lei⁴, ZHU Qing-chao³, ZHANG Yan¹, YANG Yu-chun², YANG Li-xue^1*

¹Ministry of Education Key Laboratory of Sustainable Forest Ecosystem Management, School of Forestry, Northeast Forestry University, Harbin 150040, China;
²Jilin Academy of Forestry, Changchun 130033, China;
³Afforestation Department of A'longshan Forestry Bureau, Hulunbeir 022362, Inner Mongolia, China;
⁴Disease and Pest Prevention and Quarantine Station, A'longshan Forestry Bureau, Hulunbeir 022362, Inner Mongolia, China

Received:2020-03-01 Revised:2020-06-02 Online:2020-08-15 Published:2021-02-15
Supported by:
This work was supported by the Fundamental Research Funds for the Central Universities (2572019AA07, 2572019CP16), the National Key R&D Program of China (2017YFD0601204), and the Heilongjiang Touyan Innovation Team Program (Technology Development Team for High-efficient Silviculture of Forest Resources).

摘要/Abstract

摘要： 研究了大兴安岭北部奥克里堆山不同海拔(750～1420 m)天然林土壤胞外酶活性(EEA)和酶计量比的变化特征及影响机制。结果表明: 海拔、季节及其交互作用对β-葡萄糖苷酶(BG)、N-乙酰-β-氨基葡萄糖苷酶(NAG)、亮氨酸氨基肽酶(LAP)和酸性磷酸酶(AP)活性均存在显著影响。5月,BG和NAG活性随海拔升高呈逐渐增大趋势,AP活性随海拔升高呈先升高后降低的趋势。7月,NAG活性随海拔升高而增大,AP活性则先升高后降低。9月,不同海拔上NAG活性变化幅度较大,在1420 m处活性最高,为124.22 nmol·h^-1·g^-1。随着海拔的升高,酶化学计量比ln(BG)∶ln(NAG+LAP)呈降低的趋势。除海拔830 m外,7月化学计量比值最高。土壤C、N、P转化酶活性对数转换后的比值为1∶1.25∶0.82。海拔和土壤温度是影响土壤胞外酶的主要因素,土壤温度与BG、NAG和AP呈显著正相关。ln(BG)∶ln(NAG+LAP)和ln(NAG+LAP)∶ln(AP)与pH分别呈显著正相关和负相关,与DOC分别呈显著负相关和正相关,而ln(BG)∶ln(AP)受土壤容重的影响较大。

关键词: 土壤胞外酶, 酶化学计量, 大兴安岭, 海拔

Abstract: In this study, we examined the characteristics and influence mechanism of soil extracellular enzyme activity (EEA) and enzymatic stoichiometry in different soils in forests at different altitudes (750-1420 m) in Aokelidui Mountains in the north of the Greater Khingan Mountains. The results showed that altitude, season and their interactions significantly affected the activities of β-glucosidase (BG), β-1,4-N-acetylglucosaminidase (NAG), L-leucine aminopeptidase (LAP), and acid phosphatase (AP). In May, BG and NAG activities gradually increased with increasing altitude, while AP activities increased first and then decreased with increasing altitude. In July, NAG activity increased with altitude, while AP activity increased first and then decreased. In September, NAG activity changed significantly in different altitudes, with the highest activity at 1420 m (124.22 nmol·h^-1·g^-1). With the increases of altitude, ln(BG): ln(NAG+LAP) showed a decreasing trend. Except for the altitude of 830 m, stoichiometric ratio in all altitudes was the highest in July. The ratio of logarithmic conversion of soil C, N, and P invertase activity was 1:1.25:0.82. Altitude and soil temperature were the main factors affecting soil extracellular enzyme activities. There was a significant positive correlation between soil temperature and BG, NAG, and AP. Enzymatic stoichiometry ln(BG):ln(NAG+LAP) and ln(NAG+LAP):ln(AP) showed significant positive and negative correlations with soil pH, and had a negative and positive relationship with DOC. The ratio of ln(BG):ln(AP) was greatly affected by soil bulk density.

Key words: soil extracellular enzyme, enzymatic stoichiometry, Greater Khingan Mountains, altitude

及利, 马立新, 程政磊, 祝清超, 张岩, 杨雨春, 杨立学. 大兴安岭北部不同海拔天然林土壤胞外酶化学计量特征及其季节动态[J]. 应用生态学报, 2020, 31(8): 2491-2499.

JI Li, MA Li-xin, CHENG Zheng-lei, ZHU Qing-chao, ZHANG Yan, YANG Yu-chun, YANG Li-xue. Stoichiometry of soil extracellular enzymes and its seasonal variation in natural forests with different altitudes in northern Greater Khingan Mountains, China[J]. Chinese Journal of Applied Ecology, 2020, 31(8): 2491-2499.

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大兴安岭北部不同海拔天然林土壤胞外酶化学计量特征及其季节动态

Stoichiometry of soil extracellular enzymes and its seasonal variation in natural forests with different altitudes in northern Greater Khingan Mountains, China

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