茉莉酸甲酯对菊花抗蚜性的影响

doi:10.13287/j.1001-9332.202012.039

摘要/Abstract

摘要： 本研究以杭白菊为试验材料,分析茉莉酸甲酯对菊花抗蚜性的影响。供试幼苗叶面喷施不同浓度(0.01、0.05、0.1、0.5、1 mmol·L^-1)茉莉酸甲酯后接种菊姬长管蚜,测定外源茉莉酸甲酯对蚜虫胁迫下菊花叶片的保护酶、防御酶活性、渗透性物质、次生代谢物和茉莉酸途径关键酶基因表达的影响,探究菊花抗蚜性与茉莉酸信号途径的关系。结果表明: 0.01、0.05、0.1、0.5、1 mmol·L^-1浓度的茉莉酸甲酯均显著提高了杭白菊叶片的保护酶、防御酶活性及次生代谢物含量,降低了丙二醛和可溶性糖含量,外源茉莉酸甲酯处理诱导杭白菊CmAOS和CmCOI1的表达,并使内源茉莉酸含量显著增加,杭白菊的抗蚜性增强。

关键词: 杭白菊, 茉莉酸甲酯, 抗蚜性, 茉莉酸信号途径

Abstract: We examined the effects of methyl jasmonate on aphid resistance of Chrysanthemum ‘Hangbai'. Leaves of chrysanthemum cuttings were sprayed by methyl jasmonic with different concentrations (0.01, 0.05, 0.1, 0.5 and 1 mmol·L^-1) and then inoculated with the aphids Macrosiphoniella sanborni. The following parameters are measured, including protective enzymes activities, defense enzyme activities, osmotic substances, secondary metabolites, and key genes of the jasmonic signaling pathway. The relationship between aphid resistance and the jasmonic signaling pathway in chrysanthemum was probed. The results showed that, under the treatments of all the five concentrations of methyl jasmonic, the activities of leaf protective enzymes and defense enzymes as well as the contents of secondary metabolite were increased in Chrysanthemum, whereas the contents of malondialdehyde and soluble sugar were reduced. Moreover, exogenous methyl jasmonate treatment induced the expression of CmAOS and CmCOI1, enhanced the content of endogenous jasmonic acid and the aphid resistance of chrysanthemum.

Key words: Hangbai chrysanthemum, methyl jasmonic, insect resistance, jasmonic signaling pathway.

樊婕, 张雪莹, 孙宪芝, 许冰雁. 茉莉酸甲酯对菊花抗蚜性的影响[J]. 应用生态学报, 2020, 31(12): 4197-4205.

FAN Jie, ZHANG Xue-ying, SUN Xian-zhi, XU Bing-yan. Effect of methyl jasmonate on aphid resistance of chrysanthemum.[J]. Chinese Journal of Applied Ecology, 2020, 31(12): 4197-4205.

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