种植阔叶树种和毛竹对土壤有机碳矿化与微生物群落结构的影响

doi:10.13287/j.1001-9332.202101.033

应用生态学报 ›› 2021, Vol. 32 ›› Issue (1): 82-92.doi: 10.13287/j.1001-9332.202101.033

种植阔叶树种和毛竹对土壤有机碳矿化与微生物群落结构的影响

方韬¹, 李永春^1*, 姚泽秀¹, 李永夫¹, 王兴萌¹, 王越¹, 俞叶飞²

¹浙江农林大学环境与资源学院/浙江省森林生态系统碳循环与固碳减排重点实验室/亚热带森林培育国家重点实验室, 杭州 311300;
²浙江省大盘山国家级自然保护区管理局, 浙江磐安 322300

收稿日期:2020-05-04 接受日期:2020-10-15 出版日期:2021-01-15 发布日期:2021-07-15
通讯作者: * E-mail: ycli@zafu.edu.cn
作者简介:方韬, 男, 1994年出生, 硕士研究生。主要从事土壤与环境微生物学研究。E-mail: 2017103161006@stu.zafu.edu.cn
基金资助:
国家自然科学基金项目(31670618)

Effects of planting broadleaf trees and Moso bamboo on soil carbon mineralization and microbial community structure

FANG Tao¹, LI Yong-chun^1*, YAO Ze-xiu¹, LI Yong-fu¹, WANG Xing-meng¹, WANG Yue¹, YU Ye-fei²

¹School of Environmental and Resource Sciences, Zhejiang Agriculture and Forestry University/Zhejiang Provincial Key Laboratory of Carbon Cycling in Forest Ecosystems and Carbon Sequestration/State Key Laboratory of Subtropical Silviculture, Hangzhou 311300, China;
²Administration Bureau of Dapanshan National Nature Reserve, Pan’an 322300, Zhejiang, China

Received:2020-05-04 Accepted:2020-10-15 Online:2021-01-15 Published:2021-07-15
Contact: * E-mail: ycli@zafu.edu.cn
Supported by:
National Natural Science Foundation of China (31670618).

摘要/Abstract

摘要： 为探明种植阔叶树种和毛竹对土壤有机碳矿化与微生物群落特征的影响,本研究通过盆栽试验和室内培养法比较分析种植香樟、木荷、青冈等阔叶树种与毛竹的土壤有机碳矿化速率和累计矿化量,并结合末端限制性片段长度多态性(T-RFLP)以及荧光定量PCR技术,分析土壤细菌、真菌群落组分与数量特征。结果表明: 与种植阔叶树种的土壤相比,种植毛竹的土壤活性碳组分和碳矿化总量显著提高,且其地下生物量高于阔叶树种。种植毛竹的土壤真菌/细菌丰度比也高于种植阔叶树种的土壤,土壤细菌群落组分对树种的响应比真菌群落更加敏感。种植毛竹的土壤真菌群落多样性显著低于阔叶树种;与细菌群落相比,真菌群落多样性与pH、有机碳含量、碳矿化速率更为相关。与阔叶树种相比,种植毛竹能显著增加土壤碳矿化总量,而毛竹土壤真菌群落结构是导致碳矿化量较高的原因之一。

关键词: 土壤碳矿化, 树种, 土壤细菌, 土壤真菌, 群落特征

Abstract: We conducted a pot experiment to investigate the effects of planting broadleaf tree species (i.e., Cinnamomum camphora, Schima superba, and Quercus glauca) and Moso bamboo (Phyllostachys edulis) on soil carbon mineralization and microbial community structure. The rates of soil carbon mineralization were measured via alkali trapping method. The structural and functional diversity of soil bacterial and fungal communities were analyzed by terminal restriction fragment length polymorphism (T-RFLP) and real-time quantitative PCR techniques. The soil planted with Moso bamboo exhibited a significantly higher carbon mineralization rate and labile carbon content than those in the soils planted with broadleaf tree species. The underground biomass of Moso bamboo was higher than that of broadleaf tree species. The soil bacterial communities were more sensitive than fungal communities to the planting of different plant species . Moreover, soil fungal diversity of Moso bamboo was distinctly different from that of broadleaf tree species. Compared to the diversity of soil bacterial communities, the diversity of soil fungal communities was more closely related with soil pH, organic carbon content, and carbon mineralization. In comparison to the broadleaf tree species, the Moso bamboo planting could substantially increase soil organic carbon minera-lization, which was affected mainly by the soil fungal community structure.

Key words: soil carbon mineralization, tree species, soil bacterial, soil fungal, community characteristics

方韬, 李永春, 姚泽秀, 李永夫, 王兴萌, 王越, 俞叶飞. 种植阔叶树种和毛竹对土壤有机碳矿化与微生物群落结构的影响[J]. 应用生态学报, 2021, 32(1): 82-92.

FANG Tao, LI Yong-chun, YAO Ze-xiu, LI Yong-fu, WANG Xing-meng, WANG Yue, YU Ye-fei. Effects of planting broadleaf trees and Moso bamboo on soil carbon mineralization and microbial community structure[J]. Chinese Journal of Applied Ecology, 2021, 32(1): 82-92.

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种植阔叶树种和毛竹对土壤有机碳矿化与微生物群落结构的影响

Effects of planting broadleaf trees and Moso bamboo on soil carbon mineralization and microbial community structure

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