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应用生态学报 ›› 2022, Vol. 33 ›› Issue (2): 551-559.doi: 10.13287/j.1001-9332.202202.032

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低氧及酸化胁迫对大黄鱼幼鱼离子调节与鳃组织结构的影响

曾姣1,2, 王倩1, 王亚冰1, 彭士明1, 陈润1,2, 马凌波1, 王翠华1*   

  1. 1中国水产科学研究院东海水产研究所, 上海 200090;
    2上海海洋大学水产与生命学院, 上海 201306
  • 收稿日期:2021-03-09 修回日期:2021-10-29 出版日期:2022-02-15 发布日期:2022-03-01
  • 通讯作者: *E-mail: wangch@ ecsf.ac.cn
  • 作者简介:曾 姣, 女, 1995年生, 硕士研究生。主要从事海洋生物学研究。E-mail: zengjiao0814@163.com
  • 基金资助:
    国家重点研发计划项目(2018YFD0900603)和中国水产科学研究院基本科研业务费(2020XT10)资助。

Effects of hypoxia and acidification stress on ion regulation and gill structure of juvenile Larimichthys crocea.

ZENG Jiao1,2, WANG Qian1, WANG Ya-bing1, PENG Shi-ming1, CHEN Run1,2, MA Ling-bo1, WANG Cui-hua1*   

  1. 1East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China;
    2College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
  • Received:2021-03-09 Revised:2021-10-29 Online:2022-02-15 Published:2022-03-01

摘要: 为探讨大黄鱼幼鱼在低氧及酸化胁迫下机体离子调节情况,本研究探讨了低氧(溶解氧量DO 3.5 mg·L-1,pH 8.1)、酸化(DO 7.0 mg·L-1,pH 7.35)以及低氧酸化协同胁迫(DO 3.5 mg·L-1,pH 7.35)对大黄鱼幼鱼鳃组织结构以及离子调节相关生理指标的影响。结果表明: 低氧胁迫下,大黄鱼幼鱼鳃Na+/K+-ATP酶活力、血清Na+、Ca2+及Cl-含量随胁迫时间的延长呈先递减后递增的趋势。酸化胁迫下,大黄鱼幼鱼鳃Ca2+-ATP酶活力、血清Na+及Ca2+含量呈先递增后递减的趋势。低氧酸化协同胁迫下,Na+/K+-ATP酶活力及Na+、K+、Ca2+含量呈先递增后递减的趋势,Ca2+-ATP酶活力、Cl-含量则呈先递减后递增的趋势。鳃组织学结果表明,低氧、酸化胁迫均导致鳃小片上皮细胞出现脱离现象,低氧酸化协同胁迫导致鳃小片上皮细胞出现增生、肥大、隆起现象。综合分析表明,低氧及酸化胁迫影响了大黄鱼幼鱼主要离子调节酶活力并对鳃组织造成了不同程度的损伤,最终导致了大黄鱼幼鱼体内离子调节失衡。

关键词: 低氧, 酸化, 大黄鱼, Na+/K+-ATP酶, Ca2+-ATP酶, 血清离子

Abstract: To investigate the ion regulation of large yellow croaker (Larimichthys crocea) under hypoxia and acidification stresses, we investigated the effects of hypoxia (dissolved oxygen DO 3.5 mg·L-1, pH 8.1), acidification (DO 7.0 mg·L-1, pH 7.35) and combined stresses of hypoxia and acidification (DO 3.5 mg·L-1, pH 7.35) on gill tissue structure and physiological indices related to ion regulation of juvenile L. croaker. The results showed that, under hypoxia stress, gill Na+/K+-ATPase activity, serum Na+, Ca2+ and Cl- contents of juvenile L. croaker decreased first and then increased. Under acidification stress, gill Ca2+-ATPase activity, serum Na+ and Ca2+ contents of juvenile L. croaker increased first and then decreased. Under the combined stresses of hypoxia and acidification, Na+/K+-ATPase activity and Na+, K+ and Ca2+ contents increased first and then decreased, while Ca2+-ATPase activity and Cl- content decreased first and then increased. The results of gill histology showed that hypoxia and acidification stresses led to the detachment of gill epithelial cells, and the combined stresses of hypoxia and acidification led to proliferation, hypertrophy and swelling of gill epithelial cells. Comprehensive analysis showed that hypoxia and acidification stress affected the activities of major ion regulatory enzymes in juvenile L. croaker and caused different degrees of damage to gill tissue, resulting in imbalanced ion regulation in juvenile L. croaker.

Key words: hypoxia, acidification, large yellow croaker, Na+/K+-ATPase, Ca2+-ATPase, serum ion