黄土高原苜蓿-粮食作物轮作下土壤细菌群落特征和生态功能预测

doi:10.13287/j.1001-9332.202204.028

应用生态学报 ›› 2022, Vol. 33 ›› Issue (4): 1109-1117.doi: 10.13287/j.1001-9332.202204.028

黄土高原苜蓿-粮食作物轮作下土壤细菌群落特征和生态功能预测

王晓菲¹, 罗珠珠^1,2*, 张仁陟^1,2, 牛伊宁², 李玲玲², 田建霞¹, 孙鹏洲¹, 刘家鹤¹

¹甘肃农业大学资源与环境学院, 兰州 730070;
²甘肃省干旱生境作物学重点实验室, 兰州 730070

收稿日期:2021-05-07 接受日期:2021-12-15 出版日期:2022-04-15 发布日期:2022-10-15
通讯作者: * E-mail: luozz@gsau.edu.cn
作者简介:王晓菲, 女, 1994年生, 硕士研究生。主要从事土壤生态研究。E-mail: 1352554359@qq.com
基金资助:
国家自然科学基金项目(31860364,41461067)、甘肃省科技计划项目(20JR5RA019)和甘肃省中央财政引导地方科技发展专项(ZCYD-2021-16)资助

Soil bacterial community characteristics and ecological function prediction of alfalfa and crop rotation systems in the Loess Plateau, Northwest China

WANG Xiao-fei¹, LUO Zhu-zhu^1,2*, ZHANG Ren-zhi^1,2, NIU Yi-ning², LI Ling-ling², TIAN Jian-xia¹, SUN Peng-zhou¹, LIU Jia-he¹

¹College of Resources and Environmental Science, Gansu Agricultural University, Lanzhou 730070, China;
²Gansu Provincial Key Laboratory of Aridland Crop Science, Lanzhou 730070, China

Received:2021-05-07 Accepted:2021-12-15 Online:2022-04-15 Published:2022-10-15

摘要/Abstract

摘要： 为揭示多年种植苜蓿后轮作不同作物对黄绵土细菌群落的影响,本研究通过布设在黄土高原雨养农业区的长期定位试验,采用16S rRNA基因高通量测序技术和PICRUSt方法探究苜蓿(LC)、苜蓿-休闲-小麦(L_FW)、苜蓿-休闲-玉米(L_FC)、苜蓿-马铃薯(LP)与苜蓿-谷子(LM)对土壤细菌群落结构和多样性的影响,并利用PICRUSt法预测了其生态功能。结果表明: 半干旱区黄绵土细菌优势门为放线菌门(20.3%～32.0%)、变形菌门(19.2%～23.0%)、酸杆菌门(12.4%～14.2%)和绿弯菌门(11.0%～12.7%),其中苜蓿翻耕轮作玉米土壤优势细菌属为芽孢杆菌属(1.9%),其余处理土壤细菌优势属均为假节杆菌属(2.5%)。多年生苜蓿翻耕轮作不同粮食作物后显著降低了放线菌门相对丰度,但显著提高了绿弯菌门和厚壁菌门的相对丰度。冗余分析表明,影响苜蓿及轮作作物土壤细菌群落结构的主要环境因子是硝态氮、铵态氮和全氮。PICRUSt功能预测结果表明,新陈代谢(78.6%～79.1%)为黄绵土细菌群落的主要功能,苜蓿翻耕轮作作物显著降低了土壤细菌碳水化合物代谢功能基因丰度,但明显提高了土壤细菌辅助因子和维生素代谢、神经退行性疾病、免疫系统等功能基因丰度。综上,多年生苜蓿翻耕轮作一年生作物改变了土壤细菌群落结构和生态功能,该结果可为黄绵土细菌群落演替特征的探索和苜蓿适宜后茬作物的确定提供理论参考。

关键词: 紫花苜蓿, 轮作, 高通量测序, 群落结构, 功能预测

Abstract: In order to understand the effects of lucerne cropping rotation on the bacterial community of loess soil, a long-term field experiment was conducted in rain-fed agricultural area of Loess Plateau. The cropping systems included continuous lucerne (Medicago sativa, LC), lucerne removed and rotated with spring wheat (Triticum aestivum, L_FW), lucerne removed and rotated with corn (Zea mays, L_FC), lucerne removed and rotated with potato (Solanum tuberosum, LP), and lucerne removed and rotated with continuous millet (Panicum miliaceum, LM). Based on 16S rRNA high-throughput sequencing technology, we investigated soil bacterial community structure and diversity in different cropping systems, and predicted ecological function using PICRUSt method. The results showed that the dominant phyla of loess soil bacteria were Actinomycetes (20.3%-32.0%), Proteobacteria (19.2%-23.0%), Acidobacteria (12.4%-14.2%) and Chloroflexus (11.0%-12.7%). The dominant genus was Bacillus (1.9%) in lucerne-corn system and Pseudarthrobacter (2.5%) in other treatments. Rotation with annual crops decreased the relative abundance of Actinobacteria and increased that of Chloroflexi and Firmicutes. Redundancy analysis showed that the main soil factors driving soil bacterial community structure were nitrate, ammonium, and total nitrogen. PICRUSt function prediction results showed that metabolism (78.6%-79.1%) was the main function of soil bacterial communities in loess soil. Rotation with continued annual crops significantly decreased the abundance of soil bacterial carbohydrate metabolism functional genes, and significantly increased the abundance of functional genes for soil bacterial cofactors and vitamin metabolism, neurodegenerative diseases, and immune system. In conclusion, lucerne removed and rotated with continuous annual crops changed soil bacterial community structure and ecological functions. This study provided theoretical reference to explore succession characteristics of soil bacteria and to select succeeding crops for alfalfa in loess soil.

Key words: Medicago sativa, rotation, high-throughput sequencing, community structure, function prediction

王晓菲, 罗珠珠, 张仁陟, 牛伊宁, 李玲玲, 田建霞, 孙鹏洲, 刘家鹤. 黄土高原苜蓿-粮食作物轮作下土壤细菌群落特征和生态功能预测[J]. 应用生态学报, 2022, 33(4): 1109-1117.

WANG Xiao-fei, LUO Zhu-zhu, ZHANG Ren-zhi, NIU Yi-ning, LI Ling-ling, TIAN Jian-xia, SUN Peng-zhou, LIU Jia-he. Soil bacterial community characteristics and ecological function prediction of alfalfa and crop rotation systems in the Loess Plateau, Northwest China[J]. Chinese Journal of Applied Ecology, 2022, 33(4): 1109-1117.

参考文献

[1] 马欣, 谢泽宇, 罗珠珠, 等. 陇中地区多年种植苜蓿后不同后茬作物的土壤有机氮特征. 中国土壤与肥料, 2020(2): 17-23
[2] 刘源, 张院萍. 全国苜蓿产业发展规划 (2016—2020). 中国畜牧业, 2017(11): 32
[3] 谭立伟. 甘肃省苜蓿草产业发展的现状与对策. 甘肃畜牧兽医, 2014, 44(11): 78-80
[4] 李争艳. 江淮地区不同年龄苜蓿及换茬后土壤质量变化特征研究. 硕士论文. 兰州: 甘肃农业大学, 2019
[5] Parlinson D, Coleman DC. Microbial communities, activity and biomass. Agriculture, Ecosystems and Environment, 1991, 34: 3-33
[6] Giller KE, Beare MH, Lavelle P, et al. Agricultural intensification, soil biodiversity and agroecosystem function. Applied Soil Ecology, 1997, 6: 3-16
[7] Ding JJ, Zhang YG, Deng Y, et al. Integrated meta-genomics and network analysis of soil microbial community of the forest timberline. Scientific Reports, 2015, 5: 7994
[8] 梁艳, 明安刚, 何友均, 等. 南亚热带马尾松-红椎混交林及其纯林土壤细菌群落结构与功能. 应用生态学报, 2021, 32(3): 878-886
[9] Creamer R, Hannula SE, Van Leeuwen JP, et al. Ecological network analysis reveals the inter-connection between soil biodiversity and ecosystem function as affected by land use across Europe. Applied Soil Ecology, 2016, 97: 112-124
[10] 梁志婷, 邓建强, 王自奎, 等. 陇东旱塬区不同粮草轮作模式下土壤细菌群落组成特征. 草业学报, 2017, 26(8): 180-191
[11] 樊军, 郝明德. 长期轮作与施肥对土壤主要微生物类群的影响. 水土保持研究, 2003, 10(1): 88-89
[12] 南镇武, 刘柱, 代红翠, 等. 不同轮作休耕下潮土细菌群落结构特征. 环境科学, 2021, 42(10): 4977-4987
[13] 尹国丽, 李亚娟, 张振粉, 等. 不同草田轮作模式土壤养分及细菌群落组成特征. 生态学报, 2020, 40(5): 1542-1550
[14] 耿德洲, 黄菁华, 霍娜, 等. 黄土高原半干旱区不同种植年限紫花苜蓿人工草地土壤微生物和线虫群落特征. 应用生态学报, 2020, 31(4): 1365-1377
[15] Mohammad B, Falk H, Forslund SK, et al. Structure and function of the global topsoil microbiome. Nature, 2018, 560: 233-237
[16] 鲍士旦. 土壤农化分析. 北京: 中国农业出版社, 2000
[17] Sinclair L, Osman OA, Bertilsson S, et al. Microbial community composition and diversity via 16S rRNA gene amplicons: Evaluating the illumine platform. PLoS One, 2015, 10: e0116955
[18] 段鹏飞, 陈彦, 张菲, 等. 芒草种植对土壤细菌群落结构和功能的影响. 应用生态学报, 2019, 30(6): 2030-2038
[19] Morgan GL, Jesse Z, Caporaso JG, et al. Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences. Nature Biotechnology, 2013, 31: 814-821
[20] 厉桂香, 马克明. 北京东灵山树线处土壤细菌的PICRUSt基因预测分析. 生态学报, 2018, 38(6): 2180-2186
[21] 姜雪薇, 马大龙, 臧淑英, 等. 高通量测序分析大兴安岭典型森林土壤细菌和真菌群落特征. 微生物学通报, 2021, 48(4): 1093-1105
[22] 马欣, 罗珠珠, 张耀全, 等. 黄土高原雨养区不同种植年限紫花苜蓿土壤细菌群落特征与生态功能预测. 草业学报, 2021, 30(3): 54-67
[23] Li X, Sun ML, Zhang HH, et al. Use of mulberry-soybean intercropping in salt-alkali soil impacts the diversity of the soil bacterial community. Microbial Biotechnology, 2016, 9: 293-304
[24] 金风霞, 麻冬梅, 刘昊焱, 等. 不同种植年限苜蓿地土壤环境效应的研究. 干旱地区农业研究, 2014, 32(2): 73-77
[25] 费裕翀, 黄樱, 张筱, 等. 不同有机肥处理对紫色土油茶林土壤微生物群落结构的影响. 应用与环境生物学报, 2020, 26(4): 919-927
[26] 赵雅姣, 刘晓静, 吴勇, 等. 西北半干旱区紫花苜蓿-小黑麦间作对根际土壤养分和细菌群落的影响. 应用生态学报, 2020, 31(5): 1645-1652
[27] 刘国红, 刘波, 林乃铨, 等. 芽孢杆菌的系统进化及其属分类学特征. 福建农业学报, 2008, 23(4): 436-449
[28] 赵娟, 杜军志, 薛泉宏, 等. 3株放线菌对甜瓜幼苗的促生与抗性诱导作用. 西北农林科技大学学报: 自然科学版, 2010, 38(2): 109-116
[29] 刘秀花, 梁峰, 刘茵, 等. 河南省土壤中芽孢杆菌属资源调查. 河南农业科学, 2006(8): 67-71
[30] Wu ZX, Hao ZP, Sun YQ, et al. Comparison on the structure and function of the rhizosphere microbial community between healthy and root-rot Panax notoginseng. Applied Soil Ecology, 2016, 107: 99-107
[31] Fierer N, Lauber CL, Ramirez KS, et al. Comparative metagenomic, phylogenetic and physiological analyses of soil microbial communities across nitrogen gradients. ISME Journal, 2012, 6: 1007-1017
[32] 李明, 毕江涛, 王静. 宁夏不同地区盐碱化土壤细菌群落多样性分布特征及其影响因子. 生态学报, 2020, 40(4): 1316-1330
[33] 丁钰珮, 杜宇佳, 高广磊, 等. 呼伦贝尔沙地樟子松人工林土壤细菌群落结构与功能预测. 生态学报, 2021, 41(10): 4131-4139
[34] 杨盼, 翟亚萍, 赵祥, 等. 丛枝菌根真菌和根瘤菌互作对苜蓿根际土壤细菌群落结构的影响及PICRUSt功能预测分析. 微生物学通报, 2020, 47(11): 3868-3879
[35] 吕梦超. 紫花苜蓿化学成分研究及其抗神经退行性疾病作用机制的网络药理学初探. 硕士论文. 沈阳: 沈阳化工大学, 2019
[36] 孙晓, 林余霖, 李葆莉, 等. 干旱区沙生药用植物锁阳土壤微生物群落分析与功能预测. 药学学报, 2020, 55(6): 1334-1344
[37] 孙冰洁, 张晓平, 贾淑霞. 农田土壤理化性质对土壤微生物群落的影响. 土壤与作物, 2013, 2(3): 138-144
[38] Williams MA, Rice CW. Seven years of enhanced water availability influences the physiological, structural, and functional attributes of a soil microbial community. Applied Soil Ecology, 2007, 35: 535-545

黄土高原苜蓿-粮食作物轮作下土壤细菌群落特征和生态功能预测

Soil bacterial community characteristics and ecological function prediction of alfalfa and crop rotation systems in the Loess Plateau, Northwest China

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	李长青, 纪萌, 马萌萌, 王硕, 刘欢, 孙志梅. 天然增效剂与化学抑制剂复配对小麦/玉米轮作体系产量、氮素利用及氮平衡的影响 [J]. 应用生态学报, 2023, 34(9): 2391-2397.
[2]	黄睿智, 王奇, 孙婧依, 杨绍微, 赵倚霈, 刘建锋, 肖文发. 太白山南北坡栎类林物种组成与群落特征比较 [J]. 应用生态学报, 2023, 34(8): 2055-2064.
[3]	黄庆阳, 谢立红, 曹宏杰, 王立民, 杨帆, 王继丰, 刘赢男, 倪红伟. 细菌对五大连池火山森林凋落物早期分解的影响 [J]. 应用生态学报, 2023, 34(7): 1941-1948.
[4]	郭蓉, 吴旭东, 王占军, 蒋齐, 俞鸿千, 贺婧, 刘文娟, 马琨. 荒漠草原土壤细菌和真菌群落对降水变化的响应 [J]. 应用生态学报, 2023, 34(6): 1500-1508.
[5]	刘珊珊, 王全成, 史加勉, 刘子恺, 沈菊培, 贺纪正, 郑勇. 亚热带森林菌根植物根系真菌群落结构对氮磷添加的响应 [J]. 应用生态学报, 2023, 34(6): 1547-1554.
[6]	丁爽, 魏圣钊, 陈真亮, 邵婧, 段逢瑞, 严禹, 段兴武. 中国西南典型森林土壤微生物在不同土壤深度下的变化特征 [J]. 应用生态学报, 2023, 34(3): 614-622.
[7]	余安卫, 胡汶廷, 吴思颖, 尹海锋, 范川, 李贤伟. 目标树经营对马尾松人工林不同土层深度土壤线虫群落结构的影响 [J]. 应用生态学报, 2023, 34(2): 359-368.
[8]	江尚焘, 栗晗, 彭海英, 梅新兰, 陈廷速, 徐阳春, 董彩霞, 沈其荣. 有机肥替代部分化肥对芒果丛枝菌根真菌群落的影响 [J]. 应用生态学报, 2023, 34(2): 481-490.
[9]	时一平, 宋彦涛, 那木汗, 彭擎天, 吕林有, 申悦, 倩娜, 乌云娜. 羊草和紫花苜蓿人工草地光合特性 [J]. 应用生态学报, 2023, 34(10): 2655-2662.
[10]	汪雪, 刘晓静, 王静, 童长春, 吴勇. 紫花苜蓿-燕麦连续间作下根系及土壤养分时空变化特征 [J]. 应用生态学报, 2023, 34(10): 2683-2692.
[11]	王星, 杨腾, 毛子昆, 蔺菲, 叶吉, 房帅, 戴冠华, 胡家瑞, 郝占庆, 王绪高, 原作强. 长白山阔叶红松林优势树种叶际真菌群落结构 [J]. 应用生态学报, 2022, 33(9): 2405-2412.
[12]	高慧芳, 孟婷, 熊琦, 章鸿宇, 邱君志, 林文雄, 张燎原. 太子参不同休耕年限土壤理化特征和微生物群落变化 [J]. 应用生态学报, 2022, 33(8): 2196-2204.
[13]	杨贵森, 张志山, 赵洋, 石亚飞, 虎瑞. 沙坡头地区凋落物分解及其对土壤微生物群落的影响 [J]. 应用生态学报, 2022, 33(7): 1810-1818.
[14]	窦永静, 王让虎, 吴东辉. 冻融作用对大兴安岭多年冻土区土壤节肢动物的影响 [J]. 应用生态学报, 2022, 33(5): 1405-1412.
[15]	高贝, 胡艳宇, 张志委, 丁聪, 杨雁茹, 吕晓涛. 氮素添加对呼伦贝尔草甸草原植物氮钾元素含量和计量比的影响 [J]. 应用生态学报, 2022, 33(4): 981-987.