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不同类型镉积累水稻细胞镉化学形态及亚细胞和分子分布

于辉1;杨中艺2;杨知建1 ;向佐湘1   

  1. 1湖南农业大学农学院, 长沙 410128;2中山大学生命科学学院, 广州 510275
  • 收稿日期:2008-02-04 修回日期:1900-01-01 出版日期:2008-10-20 发布日期:2008-10-20

Chemical forms and subcellular and molecular distribution of Cd in two Cd-accumulation rice genotypes.

YU Hui1; YANG Zhong-yi2; YANG Zhi-jian1; XIANG Zuo-xiang1   

  1. 1College of Agronomy, Hunan Agricultural University, Changsha 410128, China;2School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China
  • Received:2008-02-04 Revised:1900-01-01 Online:2008-10-20 Published:2008-10-20

摘要: 利用水培试验结合亚细胞组分分级分离和凝胶过滤等技术,研究了水稻根和叶中镉的化学结合形态及其亚细胞和分子分布,比较了低镉积累品种“广源占No.3”和高镉积累品种 “珍桂矮”的差异.结果表明:随着营养液中镉浓度的升高,根和叶亚细胞镉含量显著上升,大部分镉积累在细胞壁(F)和细胞可溶部分(F).高镉积累品种“珍桂矮”根和叶中可溶部分镉含量显著高于低镉积累品种“广源占No.3”.根和叶各种形态镉中,以氯化钠提取态占优势,其次是醋酸提取态,盐酸提取态镉含量最低.与“广源占No.3”相比,“珍桂矮”中迁移性较强的去离子水和乙醇提取态镉比例较高.凝胶过滤结果表明,两种类型的水稻可溶部分镉的出峰位置与样品流份中可溶性蛋白的出峰位置大致相同.可溶部分中的镉大多与分子量为3kD的物质结合,属于植物鳌合肽(PCs)或低分子量物质.“广源占No.3” 根系中镉与PCs配合的组分(Cd-PCs)含量远小于“珍桂矮”.“广源占No.3”细胞可溶部分较低的镉含量以及根系中较少的Cd-PCs形成量,降低了镉的移动及其向地上部转运的可能性.

关键词: 城镇化, 生境质量, 景观格局, 地理加权回归(GWR), 长春市

Abstract: A hydroponic experiment combined with subcellular fractionation and gel filtration was carried out to study the chemical forms and subcellular and molecular distribution of Cd in rice roots and leaves, and to compare the differences between the rice varieties ‘Guangyuanzhan No.3’ (a low-Cd accumulation genotype) and ‘Zhenguiai’ (a high-Cd accumulation genotype). The results showed that with increasing concentration of Cd in the medium, the Cd content in all fractions of rice roots and leaves increased significantly, and most of Cd accumulated in F(cell wall) and F(soluble substance). Compared with ‘Guangyuanzhan No.3’, ‘Zhenguiai’ had a significantly higher soluble fraction of Cd in its roots and leaves. The greatest amount of Cd in roots and leaves was in the extraction solution of 1 mol·L-1 NaCl, followed by in 2% HAC, and 0.6 mol·L-1 HCl. In comparison with ‘Guangyuanzhan No.3’, ‘Zhenguiai’ had a higher proportion of d-H2O and 80% ethanol extractable-Cd, the more mobile form of Cd. In the two rice varieties, the soluble Cd eluted followed the same pattern as the soluble protein in the sample medium, and the majority of soluble Cd was bounded to phytochelatins (PCs) or low molecular weight components. However, the Cd-PCs content in ‘Guangyuanzhan No.3’ roots was much lower than that in ‘Zhenguiai’ roots, which could possibly result in a lower Cd mobility in roots and less Cd translocation from root to aboveground part.

Key words: geographically weighted regression (GWR), Changchun City, landscape pattern, habitat quality, urbanization