关键词: 水稻, 印楝素, 褐飞虱, 虫害诱导的挥发物, 融雪水, 入渗, 水量平衡, 筒测法

Abstract: With the method of solid phase microextraction (SPME), a total of twenty-five volatiles were collected from rice  plants  induced by Nilaparvata lugens, and  after applying azadirachtin  fourteen of them were qualitatively identified by gas chromatography coupled by mass spectrometry (GC-MS), mainly of nine kinds of sesquiterpenes. Comparing with healthy rice plants, the plants attacked by N. lugens had more kinds of volatiles, including limonene, linalool, methyl salicylate, unknown 6, unknown 7, zingiberene, nerolidol, and hexadecane. Applying azadirachtin did not result in the production of new kind volatiles, but affected the relative concentrations of the volatiles induced by N. lugens. The proportions of limonene, linalool, methyl salicylate, unknown 6, zingiberene, and hexadecane changed obviously with the concentration of applied azadirachtin, while those of methyl salicylate, unknown 6, unknown 7, zingiberene, and nerolidol changed significantly with the days after azadirachtin application. Azadirachtin concentration, rice variety, and N. lugens density had significant interactions on the relative concentrations of all test N. lugens-induced volatiles.

Key words: Nilaparvata lugens, herbivore-induced volatile, ice, azadirachtin, snowmelt water, infiltration, water balance, cylinder method.