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应用生态学报 ›› 2010, Vol. 21 ›› Issue (10): 2529-2535.

• 研究报告 • 上一篇    下一篇

高温胁迫下外源NO对高灌蓝莓PSⅡ光化学活性和抗氧化系统的影响

魏海蓉1,孟艳玲2,孙 阳3,刘庆忠1**   

  1. 1山东省果树研究所,山东泰安 271000;2威海市农业科学院 ,山东威海 264200;3辽宁省经济林研究所,辽宁大连 116031
  • 出版日期:2010-10-18 发布日期:2010-10-18

Effects of exogenous nitric oxide on highbush blueberry PSⅡ photochemical activity and antioxidant system under high temperature stress.

WEI Hai-rong1, MENG Yan-ling2, SUN Yang3, LIU Qing-zhong1   

  1. 1Shandong Institute of Pomology, Tai’an 271000, Shandong, China;2Weihai Academy of Agricultural Science, Weihai 264200, Shandong, China;3Liaoning Institute of Economic Forestry, Dalian 116031, Liaoning, China
  • Online:2010-10-18 Published:2010-10-18

摘要: 以高灌蓝莓品种‘都克’试管苗移栽到田间6个月的植株为试材,研究了高温胁迫条件下,外源一氧化氮(NO)对高灌蓝莓幼苗生长、PSⅡ光化学活性和抗氧化系统的影响.结果表明:0.2、0.5和1.0 mmol·L-1外源NO供体硝普钠(SNP)处理显著减缓了高温胁迫对高灌蓝莓光合系统Ⅱ(PSⅡ)的抑制,高灌蓝莓PSⅡ最大光化学效率(Fv/Fm)、实际光化学效率(ΦPSⅡ)、光化学猝灭系数(qP)和非光化学猝灭系数(NPQ)下降缓慢,使光合机构免受高温胁迫的伤害;叶片质膜透性和丙二醛含量降低,而超氧化物歧化酶和过氧化氢酶活性升高,从而促进了脯氨酸的积累.适当浓度的SNP能显著缓解高温胁迫对高灌蓝莓植株的伤害,其中0.5 mmol·L-1 SNP的缓解效果最好.

关键词: 高温胁迫, 一氧化氮, 高灌蓝莓, PSⅡ光化学活性, 抗氧化系统, 叶性状, 植被带, 生活对策, 标准化主轴分析, 策略位移

Abstract: Taking the test tube ‘Duke’ highbush blueberry (Vaccinium corymbosum) seedlings having been transplanted to the field for 6 months as test materials, this paper studied the effects of exogenous nitric oxide (NO) on their growth, PSⅡ photochemical activity, and antioxidant system under high temperature stress. Applying 0,2, 0,5, and 1,0 mmol·L-1 of exogenous sodium nitroprusside (SNP) could alleviate the decrease of maximum photochemical efficiency (Fv/Fm), actual photochemical efficiency under light(ΦPSⅡ), photochemical quench (qP), and nonphotochemical quench (NPQ) caused by high temperature, and prevented the damage of high temperature on photosynthetic apparatus. Comparing with the control, treatments NO decreased the leaf membrane permeability and MDA content, increased the SOD and CAT activities significantly, and promoted proline accumulation. Appropriate concentration SNP could significantly alleviate the damage of high temperature stress on highbush blueberry seedlings, and 0.5 mmol·L-1 of SNP had the most satisfactory effect.

Key words: high temperature stress, nitric oxide (NO), highbush blueberry, PSⅡ photochemical activity, antioxidant system, leaf trait, vegetation zone, life strategy, standardized major axis test, strategy displacement.