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应用生态学报 ›› 2010, Vol. 21 ›› Issue (10): 2645-2651.

• 研究报告 • 上一篇    下一篇

集约种植雷竹林土壤细菌群落结构的演变及其影响因素

秦 华1**,李国栋2,叶正钱1,徐秋芳1,曹志洪3   

  1. 1浙江农林大学环境科技学院,浙江临安 311300;2浙江农林大学林业与生物技术学院,浙江临安 311300;3中国科学院南京土壤研究所,南京 210008
  • 出版日期:2010-10-18 发布日期:2010-10-18

Evolvement of soil bacterial community in intensively managed Phyllostachys praecox stand and related affecting factors.

QIN Hua1, LI Guo-dong2, YE Zheng-qian1, XU Qiu-fang1, CAO Zhi-hong3   

  1. 1School of Environmental Science and Technology, Zhejiang A &|F University, Lin’an 311300, Zhejiang, China|2School of Forestry and Bio-technology, Zhejiang A &F University, Lin’an 311300, Zhejiang, China;3Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China
  • Online:2010-10-18 Published:2010-10-18

摘要: 应用PCR-DGGE技术研究了雷竹林种植过程中土壤细菌群落结构的变化,并运用冗余分析(RDA)手段提取雷竹林土壤细菌群落多样性的干扰控制因子.结果表明:水稻田改种雷竹后,土壤细菌群落的Shannon指数及丰富度指数均显著增加;长期的集约种植过程对雷竹林土壤细菌群落结构产生了影响,主要细菌种群发生了变化,多样性指数表现为先增加、后期大幅降低的趋势.RDA分析表明,土壤pH、总氮、碱解氮以及速效钾这4个变量能够解释高达76.1%的样本总变异,其中土壤pH对细菌群落结构影响最大,但未达到显著水平,表明长期集约种植的雷竹林土壤细菌群落结构的变化是多种环境因子共同作用的结果.

关键词: 集约种植, 雷竹林, 土壤细菌群落, PCR-DGGE, 冗余分析, 保水剂, 冬小麦, 生长, 产量, 水分利用效率, 根形态

Abstract: By using PCR-DGGE and redundancy analysis (RDA), this paper studied the variations of soil bacterial community structure during the intensive management of Phyllostachys praecox stand, and analyzed the soil factors affecting the diversity of the bacterial community. The results showed that after the transformation of paddy field into P. praecox stand, the Shannon and richness indices of soil bacterial community increased significantly. Long-term intensive management of P. praecox stand altered the community structure of soil bacteria, reflecting in the changes of main bacterial populations and the great decrease after an initial increase of the diversity indices of soil bacterial community. Soil pH, soil total nitrogen, and soil available nitrogen and potassium could explain  76.1% of the total variation of soil bacterial community, and soil pH showed the greatest effect though it was not significant, indicating that the evolvement of soil bacterial community under the long-term intensive management of P. praecox stand was the result of the synergistic effects of several soil factors.

Key words: intensive management, Phyllostachys praecox stand, soil bacteria community, PCR-DGGE, redundancy analysis, water retentive agent, winter wheat, growth, yield, water use efficiency, root morphology.