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应用生态学报 ›› 2011, Vol. 22 ›› Issue (05): 1280-1286.

• 研究报告 • 上一篇    下一篇

共代谢条件下光合细菌对2-氯苯酚的生物降解

董怡华1,2,胡筱敏2**,和英滇2,李亮2   

  1. 1沈阳大学生物与环境工程学院, 沈阳 110044;2东北大学资源与土木工程学院, 沈阳 110004
  • 出版日期:2011-05-18 发布日期:2011-05-18

Biodegradation of o-chlorophenol by photosynthetic bacteria under co-metabolism.

DONG Yi-hua1,2, HU Xiao-min2, HE Ying-dian2, LI Liang2   

  1. 1College of Biological and Environmental Engineering, Shenyang University, Shenyang 110044, China|2College of Resources and Civil Engineering, Northeastern University, Shenyang 110004, China
  • Online:2011-05-18 Published:2011-05-18

摘要: 光合细菌PSB-1D不能利用2-氯苯酚(2-CP)作为唯一的碳源和能源.选用苹果酸、丙酸钠、乙酸钠、柠檬酸钠、苯酚、葡萄糖和可溶性淀粉等7种不同碳源作为光合细菌PSB-1D降解2-CP的共代谢基质,考察了在黑暗好氧培养条件下,不同共代谢基质对PSB-1D生长及降解2-CP效果的影响.结果表明:葡萄糖能够很好地促进PSB-1D的大量繁殖,提高降解效果,缩短降解周期,为最佳共代谢基质.对葡萄糖的投加浓度进行了优化,当葡萄糖的投加浓度为3 g·L-1时,菌株PSB-1D培养168 h后的菌体生长浓度ΔD560为1.749,2-CP的半衰期为3.9 d,降解速率常数为0.00864 h-1.采用SDS-PAGE对微生物全细胞蛋白质进行分析发现,在共代谢过程中当菌株PSB-1D利用葡萄糖作为底物提供能源和碳源时,可诱导产生2-CP特异性降解酶.

关键词: 光合细菌, 2-氯苯酚, 共代谢, 生物降解, 降解动力学

Abstract: Photosynthetic bacterial strain PSB-1D cannot utilize o-chlorophenol (2-CP) as the sole carbon source for energy. In this paper, different carbon sources (malic acid, sodium propionate, sodium acetate, sodium citrate, phenol, glucose, and soluble starch) were taken as the co-metabolism substrates to study their effects on PSB-1D growth and 2-CP degradation under the condition of aerobic culture in darkness. Among the substrates, glucose was most efficient, which promoted the reproduction of PSB-1D,enhanced the 2-CP degradation efficiency, and shortened the degradation period. The optimization experiment of added concentration of glucose showed that when the added glucose concentration was 3g·L-1, the PSB-1D cell concentration ΔD560 after 168 h culture was 1.749, the half-time of 2-CP was shortened to 3.9 d, and the degradation rate constant was increased to 0.00864 h-1. The SDS-PAGE analysis on the total microbial cellular protein showed that taking glucose as the co-metabolism substrate, PSB-1D could induce a specific 2-CP-degrading enzyme.

Key words: photosynthetic bacteria, o-chlorophenol, co-metabolism, biodegradation, degradation kinetics