外源水杨酸对辣椒倍半萜环化酶基因表达及抗氧化酶系的作用

摘要/Abstract

摘要： 分析了外源水杨酸对辣椒叶片倍半萜环化酶基因表达及抗氧化酶系的作用.结果表明,在0.5～4mmol·L^-1的浓度范围内,SA处理均能不同程度地诱导辣椒叶片中倍半萜环化酶基因转录并表达酶活性,但是酶活性较低且在SA处理36h后才出现;SA处理后,辣椒叶片SOD和POD酶活性较对照增高,CAT酶活性较对照降低,相应地,H₂O₂浓度升高.H₂O₂含量的升高与SA对辣椒叶片抗氧化酶活性的综合影响有关.

关键词: 辣椒, 倍半萜环化酶, 水杨酸, 抗氧化酶, 濒危, 生殖生态, 甘南红景天

Abstract: An analysis of the induction of sesquiterpene cyclase gene expression and antioxidant enzymes in Capsicum annuum by exongenous salicylic acid (SA) pointed out that the sesquiterpene cyclase gene expression in detached pepper leaves was induced by 0.5～4mmol·L^-1 of exongenous SA,whereas the enzyme activity and accumulation of cyclase mRNAwere much lower,and it took a longer time (36h) for the gene to be induced to express after SAtreatment,comparing to other elicitors such as UVand fungi treatment.The activities of SODand PODwere enhanced,while the CATactivity was inhibited to some degree. Consequently,H₂O₂ content was increased in SAtreated pepper leaves.H₂O₂ accumulation was related to the integrated influences from antioxidant enzymes.

Key words: Capsicum annum, Sesquiterpene cyclase, Salicylic acid, Antioxidant enzymes, Rhodiola gannanica, endangered, reproductive ecology.

中图分类号:

Q786
S641.3

何水林, 林文雄, 陈如凯. 外源水杨酸对辣椒倍半萜环化酶基因表达及抗氧化酶系的作用[J]. 应用生态学报, 2002, (5): 569-572.

HE Shuilin, LIN Wenxiong, CHEN Rukai . Induction of sesquiterpene cyclase gene expression and antioxidant enzyems regulated by exongenous salicylic acid in leaves of Capsicum annuum[J]. Chinese Journal of Applied Ecology, 2002, (5): 569-572.

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