苦槛蓝提取物对小菜蛾的生物活性

应用生态学报 ›› 2004, Vol. ›› Issue (7): 1171-1173.

苦槛蓝提取物对小菜蛾的生物活性

谷文祥, 何衍彪, 何庭玉, 庞雄飞, 冼继东

华南农业大学, 广州 510642

收稿日期:2002-07-10 修回日期:2003-12-02
通讯作者: 谷文祥,男,1954年生,教授,长期从事有机合成和天然有机化学方面的研究,已发表文章40多篇.Tel:020-85280319;E-mail:wenxgu@scau.edu.cn
基金资助:
中国科学院上海有机研究所生命有机重点实验室基金(2001.72002.7);华南农业大学校长基金资助项目(4900K990192)

Bioactivity of Myoporum bontioides extracts to Plutella xylostella

GU Wenxiang, HE Yanbiao, HE Tingyu, PANG Xiongfei, XIAN Jidong

South China Agricultural University, Guangzhou 510642, China

Received:2002-07-10 Revised:2003-12-02

摘要/Abstract

摘要： 以干扰作用控制指数(ⅡPC)为评价指标,小菜蛾为试虫,分别对苦槛蓝茎叶的不同溶剂提取物进行了生物活性测定.结果表明,石油醚和氯仿萃取部分具有较强的生物活性,而乙酸乙酯和乙醇萃取部分活性较低.在0.01 gDW·ml^-1时,用石油醚和氯仿萃取物对小菜蛾处理1 d后的产卵忌避率(ODR)分别为84.69%、79.90%,d后为76.47%、45.70%,ⅡPC为0.1565和0.2055.在0.05 gDW·ml^-1·L^-1时,1 d后为88.52%和72.25%, 3d后为87.33%和58.37%,ⅡPC为0.1125和0.2620.进而对氯仿部分进行了柱层析分离,并鉴定了3种黄酮类化合物,它们分别是5,7-二羟基黄烷酮(Ⅰ)、5,7-二羟基黄酮醇(Ⅱ)、,4'-二甲氧基-5,7-二羟基黄酮醇(Ⅲ),其中Ⅱ在生测中表现出较好的活性.

关键词: 苦槛蓝, 小菜蛾, 生物活性, 黄酮类化合物

Abstract: The bioactivity of Myopdrum bontioides extracts to Plutella xylostella was studied with IIPC as evaluated index.The results showed that petroleum ether and chloroform extracts had a higher activity than ethyl acetate and alcohol extracts.At 0.01gDW·ml^-1,the ODR of petroleum ether and chloroform extracts was 84.69% and 79 90%,and 76.47% and 45.70% after treated 1d and 3d,while the IIPC was 0.1565 and 0.2055,respectively.Provided with a higher concentration of 0.05 gDW·ml^-1,the ODR was 88.52% and 72.25%,and 87 33%,58.37%,while the IIPC was 0.1125 and 0.2620,respectively.From the chloroform extract of Myopdrum Bontioides,three flavonoids,2,3-dihydro-5,7-dihydroxy-2-phenyl-4H-1-Benzopyran-4-one(Ⅰ),3,5,7-trihydroxy-2-phenyl-4H-1-Benzopyran-4-one(Ⅱ)and 5,7-dihydroxy-3-methoxy-2-(4-methoxyphenyl)-4H-1-Benzopyran-4-one(Ⅲ),were isolated,and their structure were identified based on the analyses of physical and spectrum data.Among these compounds,(Ⅱ)had a better bioactivity to Plutella xylostella.

Key words: Myoporum bontioides, Plutella xylostella, Bioactivity, Flavonoids

中图分类号:

谷文祥, 何衍彪, 何庭玉, 庞雄飞, 冼继东. 苦槛蓝提取物对小菜蛾的生物活性[J]. 应用生态学报, 2004, (7): 1171-1173.

GU Wenxiang, HE Yanbiao, HE Tingyu, PANG Xiongfei, XIAN Jidong. Bioactivity of Myoporum bontioides extracts to Plutella xylostella[J]. Chinese Journal of Applied Ecology, 2004, (7): 1171-1173.

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