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应用生态学报 ›› 2012, Vol. 23 ›› Issue (08): 2041-2048.

• 中国生态学学会2011年学术年会会议专栏 • 上一篇    下一篇

海拔对辽东栎林地土壤微生物群落的影响

张地1,2,张育新1,曲来叶1**,张霜1,2,马克明1   

  1. (1中国科学院生态环境研究中心城市与区域生态国家重点实验室, 北京 100085; 2中国科学院研究生院, 北京 100049)
  • 出版日期:2012-08-18 发布日期:2012-08-18

Effects of altitude on soil microbial community in Quercus liaotungensis forest.

ZHANG Di1,2, ZHANG Yu-xin1, QU Lai-ye1, ZHANG Shuang1,2, MA Ke-ming1   

  1. (1State Key Laboratory of Urban and Regional Ecology, Research Center for EcoEnvironmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; 2Graduate University of Chinese Academy of Sciences, Beijing 100049, China)
  • Online:2012-08-18 Published:2012-08-18

摘要: 以北京东灵山辽东栎林地土壤为对象,运用氯仿熏蒸浸提法及磷脂脂肪酸分析(PLFA)法,研究林木生长季节土壤微生物群落随海拔梯度的变化特征.结果表明: 随着海拔升高, 辽东栎林土壤微生物生物量碳、氮,以及微生物各类群含量均有差异但不显著;土壤细菌/真菌升高,而革兰氏阳性菌(G+)/革兰氏阴性菌(G-)降低.土壤微生物生物量碳、氮以及细菌、真菌、G+细菌、G-细菌的含量与土壤含水量、有机碳、全氮呈显著正相关,土壤真菌含量与土壤碳氮比值呈正相关.土壤微生物群落组成结构(细菌/真菌和G+细菌/G-细菌)的变化主要受土壤温度和土壤含水量的显著影响,说明土壤微生物群落结构对环境条件的变化敏感.随着全球变暖的加剧
,暖温带辽东栎林地土壤真菌和G+细菌的比例有升高的趋势.  

关键词: 海拔, 土壤微生物生物量, 土壤微生物群落结构, PLFA, 东灵山

Abstract: Taking the Quercus liaotungensis forest soil in Dongling Mountain of Beijing as the object, and by using chloroform fumigationextraction and phospholipid fatty acid (PLFA) analysis methods, this paper studied the variation characteristics of soil microbial community along an altitudinal gradient in the tree growth season. With increasing altitude, the soil microbial biomass carbon and nitrogen and the quantities of various soil microbial groups in the forest had definite differences but not significant. The ratio of soil bacteria to  fungi increased, but the ratio of G+ to G- bacteria decreased. The soil microbial biomass carbon and nitrogen and the quantities of soil bacteria, fungi, and G+ and G- bacteria had significant positive correlations with the contents of soil moisture, organic carbon, and total nitrogen, and the quantity of soil fungi was positively correlated with soil carbon/nitrogen ratio. The variations of the soil microbial community structure (bacteria/fungi and G+/G-  bacteria) were mainly affected by soil temperature and moisture content, which meant that the soil microbial community structure was sensitive to the environmental conditions. Along with the  global warming, the proportions of soil fungi and G+ bacteria in the Q. liaotungensis forests in warm temperate zone would have an increase.

Key words: altitude, soil microbial biomass, soil microbial community structure, PLFA, Dongling Mountain.