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应用生态学报 ›› 2012, Vol. 23 ›› Issue (08): 2055-2061.

• 中国生态学学会2011年学术年会会议专栏 • 上一篇    下一篇

油茶芽苗砧嫁接口不同发育时期差异蛋白质分析

冯金玲,杨志坚,陈辉**   

  1. (福建农林大学, 福州 350002)
  • 出版日期:2012-08-18 发布日期:2012-08-18

Analysis of differential proteins in nurse seed grafted unions of Camellia oleifera at its different developmental stages. 

FENG Jin-ling, YANG Zhi-jian, CHEN Hui   

  1. (Fujian Agriculture and Forestry University, Fuzhou 350002, China)
  • Online:2012-08-18 Published:2012-08-18

摘要: 以油茶芽苗砧嫁接口为试验材料,运用蛋白质双向电泳结合质谱技术研究嫁接口不同发育时期(嫁接后第4、8、16、22、29和35天)蛋白质组的变化.结果表明:分析获得40个差异蛋白,其中9个差异蛋白可能与芽苗砧嫁接口愈合有关.嫁接一方面刺激嫁接口基因转录和蛋白质翻译、信号传导、能量代谢、物质合成和激素调节;另一方面刺激部分酶活性,提高细胞抗性,促进油茶芽苗砧嫁接口愈合.

关键词: 油茶, 芽苗砧嫁接, 蛋白质组学, 愈合

Abstract: By using two-dimensional electrophoresis and mass spectrometry, this paper studied the changes of the proteome in nurse seed grafted unions of Camellia oleifera at its different developmental stages (4, 8, 16, 22, 29, and 35 days after grafting). A total of 40 differential proteins were detected, 9 of which could be related to the healing of nurse seed grafted unions. Grafting stimulated the grafted union’s gene transcription and protein translation, signal transduction, energy metabolism, and compound synthesis and hormone regulation, and activated some enzymes activities, enhanced cell resistance, and promoted the union’s healing.

Key words: Camellia oleifera, nurse seed grafting, proteomics, healing.