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应用生态学报 ›› 2012, Vol. 23 ›› Issue (02): 363-368.

• 研究论文 • 上一篇    下一篇

脱硫废弃物对碱胁迫下水稻叶片钙分布、Ca2+-ATPase活性及抗氧化特征的影响

毛桂莲1,许兴2**,曾瑾1,岳自慧2,杨淑娟1   

  1. 1宁夏大学生命科学学院, 银川 750021; 2宁夏大学农学院, 银川 750021
  • 出版日期:2012-02-18 发布日期:2012-02-18

Effects of desulfurization waste on calcium distribution, Ca2+-ATPase activity, and antioxidant characteristics of rice leaf under alkali stress.

MAO Gui-lian1, XU Xing2, Zeng Jin1, YUE Zi-hui2, YANG Shu-juan1   

  1. 1School of Life Science, Ningxia University, Yinchuan 750021, China; 2School of Agriculture, Ningxia University, Yinchuan 750021, China
  • Online:2012-02-18 Published:2012-02-18

摘要: 为了探讨脱硫废弃物提高水稻抗盐碱的作用机制,采用盆栽法,研究脱硫废弃物对碱胁迫下水稻幼苗叶片总钙含量、Ca2+分布、细胞膜Ca2+-ATPase活性及活性氧含量等的变化.结果表明: 对照处理的细胞中钙颗粒零星分布于细胞壁和叶绿体中,添加脱硫废弃物和CaSO4处理的细胞质膜、细胞间隙、细胞壁和液泡中有大量的钙颗粒分布;随着脱硫废弃物和CaSO4添加量的增加,叶片总钙含量增加,质膜和液泡膜Ca2+-ATPase活性呈上升趋势,质膜透性、MDA含量和活性氧O2〖SX(B-*3〗-〖〗·〖SX)〗产生速率呈下降趋势,SOD、POD等保护酶活性升高.添加脱硫废弃物在一定程度上能够减缓碱胁迫对水稻造成的细胞伤害,起主要作用的物质可能是其主要成分CaSO4.

关键词: 水稻, 碱胁迫, 脱硫废弃物, 活性氧, Ca2+

Abstract: To approach the action mechanisms of desulfurization waste on alleviating alkali stress- induced injury of rice, a pot experiment was conducted to study the variations of leaf total calcium content, calcium distribution, plasma membrane Ca2+-ATPase activity, and reactive oxygen content of rice seedlings under alkali stress after the application of desulfurization waste. In the control, a few calcium particulates scattered in the cell wall and chloroplasts, while applying desulfurization waste or CaSO4 increased the calcium particulates in the plasma membrane, intercellular space, cell wall, and vacuole significantly. With the increasing application rate of desulfurization waste or CaSO4, the leaf total calcium content increased, Ca2+-ATPase activity in plasma membrane and tonoplast presented an increasing trend, plasma membrane relative permeability, MDA content, and O2〖SX(B-*3〗-〖〗·〖SX)〗production rate decreased, and SOD and POD activities increased. The desulfurization waste could relieve the alkali stress to rice in some extent, and the main reactive compound in the waste could be CaSO4.

Key words: rice, alkali stress, desulfurization waste, reactive oxygen, Ca2+