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应用生态学报 ›› 2005, Vol. 16 ›› Issue (12): 2413-2417.

• 研究论文 • 上一篇    下一篇

铜对三叶草-土壤酶系统的影响

储玲, 王友保, 丁佳红, 李征, 刘登义   

  1. 安徽师范大学生命科学学院 重要生物资源保护与利用研究安徽省重点实验室, 芜湖 241000
  • 收稿日期:2005-01-25 修回日期:2005-05-26 出版日期:2005-12-15 发布日期:2005-12-15
  • 通讯作者: 刘登义
  • 基金资助:
    国家重点基础研究发展规划项目(2004CB418503)、国家自然科学基金项目(30470270)、安徽省教育厅自然科学基金项目(2005kj183)和安徽师范大学青年科学基金资助项目(2005xqn11).

Effects of copper pollution on Trifolium repens growth and soil enzyme activities

CHU Ling, WANG Youbao, DING Jiahong, LI Zheng, LIU Dengyi   

  1. Anhui Normal University, Wuhu 241000, China
  • Received:2005-01-25 Revised:2005-05-26 Online:2005-12-15 Published:2005-12-15

摘要: 通过盆栽实验研究了重金属Cu污染对植物(三叶草)-土壤酶(脲酶、蔗糖酶、过氧化氢酶和多酚氧化酶)系统的影响.结果表明,随着Cu浓度增加,脲酶、蔗糖酶、过氧化氢酶和多酚氧化酶活性均逐渐减小,与Cu浓度有高度相关性,蔗糖酶>多酚氧化酶>脲酶>过氧化氢酶.在处理浓度不变情况下,酶活性随时间而变化,且呈现低Cu浓度(<00 mg·kg-1)时4种酶活性均有所上升,而Cu浓度增高(00~3 000 mg·kg-1)时各酶活性逐渐下降的趋势.统计分析表明,在每一梯度浓度上,4种酶在Ⅰ、Ⅱ、Ⅲ组内均存在显著差异性(P<0.01),与植物受重金属Cu污染时的生长情况一致.随着Cu浓度增加,土壤pH值逐渐下降,而电导率上升;同一Cu浓度下的pH值和电导率均随时间呈缓慢上升趋势,统计分析显示,二者在Ⅰ、Ⅱ、Ⅲ组内均存在显著差异性(P<0.01).土壤pH值和电导率与4种土壤酶活性有高度相关性,多酚氧化酶>蔗糖酶>过氧化氢酶>脲酶.这4种酶同时可作为检测土壤环境质量的指标.

关键词: 铜, 三叶草, 生长, 土壤酶活性

Abstract: The study with pot experiment showed that with increasing Cu concentration,soil urease,invertase,catalase and polyphenol oxidase activities decreased gradually.There was a significant correlation between Cu concentration and soil enzyme activities,with the correlated degree followed the order of invertase>polyphenol oxidase>urease>catalase.Under a fixed Cu concentration,soil enzyme activities changed with time,and the changes were different between high and low Cu concentrations,being increased slightly under low Cu concentration (<500 mg·kg-1),but decreased gradually as Cu concentration increased (500~3 000 mg·kg-1).Statistical analysis indicated that within the range of test Cu concentrations,the activities of test soil enzymes were significantly different among different Cu concentration (P<0.01),which was accorded with the seedlings growth status.Soil pH was decreased,while electric conductivity was increased with increasing Cu concentration (500~3 000 mg·kg-1),but they were increased with time under a fixed Cu concentration,with significant difference among different Cu concentration (P<0.01).Soil pH and electric conductivity were highly related to soil enzyme activities,with the order of polyphenol oxidase>invertase>catalase>urease.The test soil enzyme activities could be used as the indices of soil environment quality.

Key words: Cu, Trifolium repens, Growth, Soil enzyme activity

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