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应用生态学报 ›› 2005, Vol. 16 ›› Issue (10): 1889-1892.

• 研究论文 • 上一篇    下一篇

云南四个瓜实蝇地理种群的遗传关系分析

朱振华1,2, 叶辉1, 张智英1   

  1. 1. 云南大学生命科学学院, 昆明 650091;
    2. 云南省农业科学院粮作所, 昆明 650205
  • 收稿日期:2004-09-15 修回日期:2005-02-21 出版日期:2005-10-15 发布日期:2005-10-15
  • 通讯作者: 叶辉,E mail:yehui@ynu.edu.cn
  • 基金资助:
    云南省自然科学基金项目(2003C0007M)、国家自然科学基金项目(30260023)和国家重点基础研究发展规划资助项目(2003CB415100).

Genetic relationships among four Bactrocera cucurbitae geographic populations in Yunnan Province

ZHU Zhenhua1,2, YE Hui1, ZHANG Zhiying 1   

  1. 1. College of Life Science, Yunnan University, Kunming 650091, China;
    2. Institute of Crops, Yunnan Academy of Agricultural Science, Kunming 650205, China
  • Received:2004-09-15 Revised:2005-02-21 Online:2005-10-15 Published:2005-10-15

摘要: 对云南个不同地理区域的瓜实蝇种群(Bactrocera cucurbitae)共23个个体的线粒体DNA细胞色素b基因中的部分序列进行测定和分析,并以桔小实蝇(B.dorsalis)、番石榴实蝇(B.correct)和南瓜实蝇(B.tau)为外群种构建了不同单倍型的N-J分子系统树.在获得的26bp序列中,A+T含量约占65.0%,有个多态位点,无任何碱基插入和缺失.这些位点共定义5种单倍型,其中一种为共享单倍型.对个瓜实蝇地理种群进行Fst值和基因流动统计,Fst值为0.16667~0.20000(P>0.05),Nm值为2.00~2.50.5种单倍型共形成了3个聚类簇.可以认为,个地理种群间均存在一定程度的遗传分化,但分化程度不高,导致遗传分化的主要因素是地理隔离,而种群遗传分化程度低与瓜实蝇所在环境条件相似有关.

关键词: 瓜实蝇, 线粒体DNA, 细胞色素b基因, 遗传分化

Abstract: In this study,the partial section of mitochondrial cytochrome b in 23 individuals of four Bactrocera cucurbitae geographic populations(Banna,Ruili,Yuanjiang,and Hekou)in Yunnan Province was sequenced,and the phylogenetic trees of all haplotypes were constructed by means of N-J method,using B.dorsalis,B.correct and B.tau as the out-group species.Of the 426 bp mitochondrial cytochrome b DNA sequences obtained,A%+T% was about 65.0%,four nucleotide sites were substituted,and no base composition of the sequences was inserted and lost.Five haplotypes were identified in all the sequences,with four showing polymorphism,and one shared.The genetic characters of the four populations were analyzed by Fst value and gene flow through Mega software.The Fst value was 0.16667~0.20000(P>0.05),and the Nm value was 2.00~2.50.The N-J tree suggested that four haplotypes constructed three clades.All results showed that the genetic differences presented among the four populations,but with low degrees.The main reason causing the genetic differences among the four populations was supposed to be related to genetic isolation and ecological adaptation.

Key words: Bactrocera cucurbitae, MtDNA, Cytochrome b gene, Genetic differentiation

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