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应用生态学报 ›› 2005, Vol. 16 ›› Issue (8): 1577-1580.

• 研究论文 • 上一篇    

最大或然数法在光合细菌计数中的应用及效果研究

崔战利1, 王萍萍2, 王秋菊1   

  1. 1. 黑龙江八一农垦大学生命科技学院, 大庆 163319;
    2. 沈阳农业大学基础部, 沈阳 110161
  • 收稿日期:2004-12-23 修回日期:2005-04-11 出版日期:2005-08-15 发布日期:2005-08-15
  • 通讯作者: 崔战利,女,1964年生,硕士,副教授.主要从事土壤和环境微生物研究,发表论文12篇.E-mail:zhanlic@yahoo.com.cn

Application effect of most probable number(MPN)method in photosynthetic bacteria counting

CUI Zhanli1, WANG Pingping2, WANG Qiuju 1   

  1. 1. College of Biology Science and Technology, Heilongjiang August First Land Reclamation University, Daqing 163319, China;
    2. Department of Basic Science, Shenyang Agricultural University, Shenyang 110161, China
  • Received:2004-12-23 Revised:2005-04-11 Online:2005-08-15 Published:2005-08-15

Abstract: The application of most probable number(MPN)method in counting the numbers of photosynthetic bacteria(PSB)showed that this method was suitable for PSB counting under illumination and anaerobic condition,which remedied the shortages of other methods.With this method,the sensitivity and accuracy of the counting could be enhanced when more replicated tubes and lower dilution times for fewer bacteria samples were applied.When PSB were not dominant in soil and water,MPN method could be used instead of other methods.PSB agent could only survive 69 days,and not be dominant in the environment in a long term.Its application time and oxygen level affect the numbers of PSB significantly,which were the largest in a short application time and under anaerobic condition.

Key words: Photosynthetic bacteria(PSB), Most probable number(MPN), Bacteria counting, Application effect

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