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应用生态学报 ›› 2004, Vol. ›› Issue (11): 2161-2164.

• 研究论文 • 上一篇    下一篇

水稻品种中抗褐飞虱抗原次生物质的分析

赵颖, 黄凤宽, 童晓立, 凌冰, 庞雄飞   

  1. 华南农业大学理学院应用化学系, 资源环境学院昆虫生态研究室, 广州 510642
  • 收稿日期:2003-10-12 修回日期:2004-02-28 出版日期:2004-11-15
  • 通讯作者: 赵颖,女,1967年出生,硕士,讲师,主要从事天然产物化学及化学生态方面的研究.E-mail:zhaoying@scau.edu.cn
  • 基金资助:
    国家自然科学基金重点项目(39930120);华南农业大学校长基金资助项目(2002047)

Secondary compounds in rice varieties resistant to Nilaparvata lugens

ZHAO Ying, HUANG Fengkuan, TONG Xiaoli, LING Bing, PANG Xiongfei   

  1. Department of Chemistry, College of Sciences, Laboratory of Insect Ecology, South China Agricultural University, Guangzhou 510642, China
  • Received:2003-10-12 Revised:2004-02-28 Online:2004-11-15

摘要: 借助高效液相色谱(HPLC)技术,研究了130份对褐飞虱生物型Ⅱ具有不同抗性水平的水稻样品(26个品种)中13个次生物质含量(峰面积)的差异;运用主成分分析和多元回归分析,建立了水稻品种抗性级别的预测模型:Y=3.4593-0.02491X1+0.08475X2-0.04227X8+0.1174X12.结果表明,水稻的抗性水平与峰面积值之间极显著相关(r2=0.84,P<0.01),峰1、峰2、峰8、峰12对应的次生化合物是影响水稻对褐飞虱生物型Ⅱ抗性水平的主要抗原次生化合物;水稻品种中起抗虫作用的抗原次生物质不止一种,而是几种的组合,而且它们对水稻抗虫性的贡献权重是不完全相同的.

关键词: 抗原次生物质, 水稻, 抗虫育种, 高效液相色谱, 褐飞虱

Abstract: By the method of high-performance liquid chromatography (HPLC),this paper studied the contents of 13 secondary compounds from 130 samples of 26 rice varieties resistant to brown planthopper (BPH) Nilaparvata lugens biotype Ⅱ.A prediction model was established through principal component analysis and multiple regression analysis: Y=3.4593-0.02491X1+0.08475X2-0.04227X8+0.1174X12.The relationships between the BPH-resistant level of rice varieties and the area values of chromatographic peaks were significant (r2=0.84,P<0.01).The results demonstrated that Peak 1,Peak 2,Peak 8 and Peak 12 were the major secondary compounds affecting the resistance to BPH biotype Ⅱ,which suggested that the BPH resistance of the varieties came from the action of several secondary compounds that varied in contribution to the resistance.

Key words: Resistant secondary compounds, Rice, Resistant breeding to insects, HPLC, Brown planthopper

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