球孢白僵菌Bb174固态发酵产几丁质酶产酶及酶学特征研究

应用生态学报 ›› 2004, Vol. ›› Issue (5): 863-866.

球孢白僵菌Bb174固态发酵产几丁质酶产酶及酶学特征研究

张洁¹, 蔡敬民¹, 吴克¹, 金胜先¹, 潘仁瑞¹, 樊美珍²

1. 合肥学院生物科学与技术系, 合肥 230022;
2. 安徽农业大学虫生真菌研究所, 合肥 230036

收稿日期:2002-11-12 修回日期:2003-06-16 出版日期:2004-05-15
通讯作者: 樊美珍
基金资助:
安徽省教育厅自然科学基金项目(2000jl267);合肥学院科研基金项目;安徽省优秀青年科技基金资助项目(04043051)

Production and properties of chitinase from Beauveria bassiana Bb174 in solid state fermentation

ZHANG Jie¹, CAI Jingmin¹, WU Ke¹, JIN Shengxian¹, PAN Renrui¹, FAN Meizhen ²

1. Department of Biology Science and Technology, Hefei College, Hefei 230022, China;
2. Institute of Economic Insects and Fungi, Anhui Agricultural University, Hefei 230036, China

Received:2002-11-12 Revised:2003-06-16 Online:2004-05-15

摘要/Abstract

摘要： 对球孢白僵菌(Beauveria bassiana)Bb174产几丁质酶进行了固态发酵条件及酶学特征的研究.结果表明,以4:1麸皮:蚕蛹粉、蛋白胨1g·L^-1作为产酶最适培养基,在75g培养基中接种3ml液态种子,自然pH下28℃培养2d,酶活可达最高,为126U·g^-1(干培养基).粗酶液的最适反应温度为40℃,最适反应pH5.0,在30～70℃保温1h,得半失活温度48℃.在30～40℃、pH4～6范围内,酶的性质最稳定.根据Lineweaver-Burk作图法,得到该酶的动力学参数K_m为0.52mg·ml^-1,V_m为0.7△E₆₈₀·h^-1.

关键词: 球孢白僵菌, 几丁质酶, 固态发酵, 酶动力学

Abstract: This paper studied the chitinase production of Beauveria bassiana Bb174 under solid state fermentation condition.The optimal medium consisted of wheat bran and silkworm chrysalis at the ratio of 4:1,supplemented with 1 g peptone·L^-1 as nitrogen source and some other mineral nutrients.The enzyme activity reached 126 units per gram dry medium after cultured for 2 days at 28 ℃ and natural pH by inoculated 3 ml spore suspension into this medium.The optimal temperature and pH for chintinase production were 40 ℃ and 5.0,respectively.The temperature to lose 50% activity of the enzyme was 48 ℃ after incubated at 30～70 ℃ for 1 h.The enzyme was stable at 30～40 ℃ and pH 4～6,and the K_m and V_max values were 0.52 mg·ml^-1 and 0.7△E₆₈₀·h^-1,respectively.

Key words: Beauveria bassiana, Chitinase, Solid state fermentation, Kinetic of enzyme reaction

中图分类号:

Q938.1

张洁, 蔡敬民, 吴克, 金胜先, 潘仁瑞, 樊美珍. 球孢白僵菌Bb174固态发酵产几丁质酶产酶及酶学特征研究[J]. 应用生态学报, 2004, (5): 863-866.

ZHANG Jie, CAI Jingmin, WU Ke, JIN Shengxian, PAN Renrui, FAN Meizhen . Production and properties of chitinase from Beauveria bassiana Bb174 in solid state fermentation[J]. Chinese Journal of Applied Ecology, 2004, (5): 863-866.

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