Abstract: Studies on the efficacy of bio-rational pesticides and agricultural methods against the chief diseases, insect pests and weeds of rice showed that the efficacy of the mixtures of jingangmycin and bacillus-cereus, and jingangmycin and polyoxin against rice sheath blight were 75.16%～94.27% after sprayed once at the tiller and boot end stages of rice, respectively, and better than that of chemical fungicide triadimefon. The efficacy of kasugamycin and blasticidin was 50.54%～72.67% on rice leaf blast and 76.66%～87.42% on rice head blast, and equal to the chemical fungicide tricyclazole after sprayed once at the initial stage of rice leaf blast occurrence and the initial and end stages of earing, respectively. The efficacy of bacillus thuringiensis on Chilo suppressalis and Cnaphalocrocis medinalis was better than that of chemical insecticide bisultap, and the efficacy of saponin-nicotine and matrine was equal to that of chemical insecticide bisultap when the three biorational insecticides were sprayed at 1～2 instar larvae of pests. The efficacy of saponin -nicotine and matrine was above 70%, and lower than that of chemical insecticide imidacloprid 3～30d after sprayed at 1～2 instar larvae of Nilaparvata lugens. The occurrence of weeds could be controlled, and the rice yield could be raised when the suitable non-thorough decomposed organism was applied or weeding was carried after the field had been ploughed twice before rice transplant. The rice yield could be raised by using biorational pesticides and agricultural methods against the chief diseases, insect pests and weeds of rice. The residue of pesticides in rice was lower in the bio-control area than in the chemical control area, according with the demands of health target of green food.