黄瓜苗根围拮抗细菌X3的分子鉴定

应用生态学报 ›› 2003, Vol. ›› Issue (9): 1521-1524.

黄瓜苗根围拮抗细菌X3的分子鉴定

沈爱华, 张炳欣, 李斌, 沈立荣, 喻景权

浙江大学植物保护系, 杭州 310029

收稿日期:2002-11-25 修回日期:2003-02-28 出版日期:2003-09-15
通讯作者: 张炳欣
基金资助:
国家自然科学基金资助项目(30070511,30230250)

Identification on an antagonistic rhizobacterium X3 from rhizosphere of cucumber

SHEN Aihua, ZHANG Bingxin, LI Bin, SHEN Lirong, YU Jingquan

Zhejiang University, Hangzhou 310029, China

Received:2002-11-25 Revised:2003-02-28 Online:2003-09-15

摘要/Abstract

摘要： 采用生理生化、Biolog和16SrDNA分子鉴定3种不同方法,对抑制黄瓜苗期猝倒病病原真菌的细菌菌株X3进行了鉴定。生理生化鉴定显示该菌株为Pseudomonas aeruginosa;而Biolog鉴定显示其为P.spinosa;进一步对该菌株作16SrDNA基因的测定与分析,表明其与已报道的P.aeruginosa 16SrDNA具有93.7%的同源性,二者在所建系统发育树中处于同一分枝,据此确定该菌株为P.aeruginosa。

Abstract: Classical physiological and biochemical methods, Biolog, and 16SrDNAanalysis were used to identify the strain X3 which could control cucumber seedling damping-off.Physiological and biochemical identification showed that it belonged to Pseudomonas aeruginosa, but Biolog identification showed it was P.spinosa.16SrDNA determination and analysis was used for further identification, which showed that the 16SrDNA sequence of X3 shared 93.7% homologies with published sequence of P.aeruginosa from GenBank, and both sequences constituted a branch in Phylogenetic tree.Based on these results, it is considered that the strain X3 belongs to one strain of P.aeruginosa.

中图分类号:

S436.21

沈爱华, 张炳欣, 李斌, 沈立荣, 喻景权. 黄瓜苗根围拮抗细菌X3的分子鉴定[J]. 应用生态学报, 2003, (9): 1521-1524.

SHEN Aihua, ZHANG Bingxin, LI Bin, SHEN Lirong, YU Jingquan . Identification on an antagonistic rhizobacterium X3 from rhizosphere of cucumber[J]. Chinese Journal of Applied Ecology, 2003, (9): 1521-1524.

参考文献

[1] Chen X-B (陈晓斌), Zhang B-X (张炳欣), Lou B-G(楼兵干), et al. 2000. Studies on identification to plant growth-Promoting rhizobacteria of cucumber using biolog analysis. Microbiology (微生物学通报), 27(6) :403～407 (in Chinese)
[2] Fang Z-D(方中达). 1998. The Methods for Studying Plant Pathology. 3rd edition. Beijing: China Agricultural Press. (in Chinese)
[3] Friedrich VW, Sebastian B, Cord S, et al. 2002. Base-specific fragmentation of amplified 16S rRNA genes analyzed by mass spectromentry: A tool for rapid bacterial identification. Microbiology,99(10) :7039～7044
[4] Fry NK, Warwick S, Saunders NA, et al. 1991. The use of 16S ribosom al RNA analyses to investigate the phylogeny of the family Legionellaceae . J Gen Microbiol,137:1215～1222
[5] Fu J-F(傅君芬),Hong W-L(洪文澜). 1998. 16s～23s Gene Sequence-A new method to classify and identify bacteria. Foreign Medic Epidemiol Fascic (国外医学流行病学传染学分册), 25(6) :245～249 (in Chinese)
[6] Jeng RS, Svircev AM, Myers AL, et al. 2001. The use of 16S and 16S-23S rDNA to easily detect and differentiate common gram-negative orchard epiphytes. J Microbiol Methods, 44: 69～77
[7] Julian RM, Takuichi S, Anderew JW, et al. 1998. Design and evaluation of useful bacterium-specific PCR primers that ampligy genes coding for bacterial 16S rRNA. Appl Environ Microbiol, 64(2): 795～799
[8] Li D-B(李德葆), Zhou X-P(周雪平), Xu J-P(许建平), et al.1996. Technology of Gene Engineer Operation. Shanghai: Shanghai Science Technology Press. (in Chinese)
[9] Lou B-G(楼兵干), Zhang B-X(张炳欣), Hu L-Q(胡利强).2001. Resistance to metalaxyl and biological control of Pythium spp. Acta Ph ytoph Sin (植物保护学报),28 (1) : 5 5～60 (in Chinese)
[10] Lou B-G(楼兵),Zhang B-X(张炳欣). 2002. Biological control of seedling damping-off of cucumber. Acta Phytoph Sin (植物保护学报), 29(2): 109～113 (in Chinese)
[11] Rupert L, Kellner L. 2002. Molecular identification of an eddosymbiotie bacterium associated with pederin biosynthesis in Paederus sabaeus. Insect Biochem Molec Biol, 32: 389～395
[12] Schtoth MN, Hancock JG. 1981. Selected topics in biological control. Ann Rev Microbiol, 35: 453～476
[13] Wang SL, Yieh TC, Shih IL. 1999. Purification and characterization of a new antifungal compound produced by Pseudomonas aeruginosa K-187 in a shirimp and crab shell powder medium. Enzyme Microb Technol, 25 :439～446
[14] Watanabe K, Kodama Y, Harayama S. 2001. Design and evaluation of PCR Primers to amplify bacterial 16S ribosomal DNA fragments used for community fingerprinting. J Microbiol Methods, 44:253～262
[15] Xie GL. 1996. Characterization of Pseudomonas spp. and other bacterial species associated with rice seeds. Laguna, Philippines:IRRI-CPLB. 166～174
[16] Yojiro AZ, Kim H, Park JY, et al. 2000. Phylogenetic affiliation of the pseudomonas based on 16S rRNA sequence. InterJ Syst Evolu Microbiol, 50:1563～1589