杭州西湖水体中异养细菌生长的限制因子

应用生态学报 ›› 2003, Vol. ›› Issue (8): 1343-1346.

杭州西湖水体中异养细菌生长的限制因子

吴根福¹, 杨志坚¹, 周雪平¹, 吴洁²

1. 浙江大学, 杭州 310028;
2. 杭州市环境保护监测站, 杭州 310007

收稿日期:2001-06-20 修回日期:2001-10-08 出版日期:2003-08-15
通讯作者: 吴根福,男,1965年生,硕士,副教授,主要从事环境微生物方面的研究工作,发表论文近30篇.E-mail:wu-genfu@zju.edu.cn.
基金资助:
浙江省自然科学基金(9515106);中日合作基金资助项目

Factors restricting growth of heterotrophic bacteria in the water body of West Lake, Hangzhou

WU Genfu¹, YANG Zhijian¹, ZHOU Xueping¹, WU Jie²

1. Zhejiang University, Hangzhou 310028, China;
2. Hangzhou Manicipal Environmental Monitoring Center, Hangzhou 310007, China

Received:2001-06-20 Revised:2001-10-08 Online:2003-08-15

摘要/Abstract

摘要： 通过添加不同营养盐类的纯种和自然培养试验,对异养细菌生长的限制因子进行了研究.结果表明,生物可利用的有机碳是主要的限制性营养因子,而氮源和磷源的影响相对较小;湖水的高pH、丰富的藻类和浮游动物生物量也制约了异养细菌的生长.此外还发现,在自然水体中添加0.5%葡萄糖后,一些自生固氮细菌得到富集;添加C+N+P后,有大量霉菌生长,添加0.01%牛肉膏后,假单胞菌属(Pseudomonas)细菌由原来的30%提高到57%;湖水进行实验室培养时细菌的最大生长量可达10⁵个·ml^-1.

Abstract: Restricting factors of bacteria growth were studied by pure culture and natural culture test. The results showed that organic carbon source available for bacteria was more important than (NH₄)₂SO₄ and KH₂PO₄, while higher pH, and rich biomass of phytoplanktons and zooplanktons in the water restrained the growth of heterotrophic bacteria. Under natural culture experiment, Azotobacter increased after 0.5% glucose was added, and a lot of mildew grew after adding0.5% glucose with 0.1%(NH₄)₂SO₄ and 0.1% KH₂PO₄, while Pseudomonas enriched 30～57% after adding0.01% beef extract. It was also showed that bacteria growth potentiality in natural water could reach to 10⁵cfu·ml^-1.

中图分类号:

Q938.8

吴根福, 杨志坚, 周雪平, 吴洁. 杭州西湖水体中异养细菌生长的限制因子[J]. 应用生态学报, 2003, (8): 1343-1346.

WU Genfu, YANG Zhijian, ZHOU Xueping, WU Jie. Factors restricting growth of heterotrophic bacteria in the water body of West Lake, Hangzhou[J]. Chinese Journal of Applied Ecology, 2003, (8): 1343-1346.

参考文献

[1] Cavallo RA, Rizzi C, Vozza T, et al. 1999. Viable heterotrophic bacteria in water and sediment in Mar Piccolo of Taronto, Italy. J Appl Microbiol, 86(6): 906～916
[2] Dong X-Z(东秀珠),Cai M-Y(蔡妙英).2001.Systematic Determination of General Bacteria. Beijing: Science Press. 128～141 (in Chinse)
[3] Iwakuma T, Nakazato R, Arakawa T. 1999. A preliminary study on the distribution of zoobenthos and zooplankton in the West Lake, Hangzhou. Rep Suwa Hydrobiol, 11:67～72.
[4] Jin X-C(金相灿),Du Q-Y(屠清瑛).1990.The Investigative Standards of Lake Eutrophication. Beijing: China Environmental Science Press. 160～265 (in Chinese)
[5] John GH. 1984. Bergey' s Manual of Systematic Bacteriology. Balti more: The Williams Wilkins Company. 141～234
[6] Li GG, Wei CD, Zhou H, et al. 1999. Ecological studies on zooplankton of West Lake and its inflows. Rep Suwa Hydrobiol, 11: 41～50
[7] Mayo AW, Noike T. 1994. Response of mixed cultures of Chlorella vulgaris and heterotrophic bacteria to variation of pH. Water Sci Technol, 30(8): 285～294
[8] Mohamed MN, Lawrence JR, Robarts RD. 1998. Phosphorus limitation of heterotrophic biofilm from the Fraser river, Britsh Columbia and the effect of pulp mill effluent. Microbiol Ecol, 36 (2): 121～130
[9] Okino T, Pei HP. 1999. Japan-China jiont project on conservation of the environment and historical landscape of West Lake, Hangzhou, China. Rep Suwa Hydrobiol , 11:43～81
[10] Oufdou K, Mezrioui N, Oudra B, et al. 1998. Experimental study of the effect of Synechocystis sp. on the behaviour of some bacteria of sanitary interest. Anns Limnol, 34(3): 259～268
[11] Sathasivan A, Ohgaki S. 1999. Application of new bacterial regrowth potential method for water distribution system. Wat Res, 33 (1): 137～144
[12] Thingstad TF, Lizweifel U, Rassoulzadegan F. 1998. Phosphorus limination of heterotrophic bacteria and phytoplankton in the northwest Mediterranean. Limnol Oceanog, 43(1): 88～94.
[13] Tranvik LJ. 1990. Bacterioplankton growth on fractions of dissolved organic carbon of different molecular weights from humicand clear waters. Appl Environ Microbiol, 56:1672～1677
[14] Wu G-F(吴根福),Xuan X-D(宣晓东),Li M-Z(李梅姿),et al.1998.Preliminary studies on release of phosphorus from the sediment of West Lake,Hangzhou.chin Environ Sci(中国环境科学),18(2):107 110(in Chinese)
[15] Wu G-F(吴根福),Xuan X-D(宣晓冬),Wang F-S(汪富三).1999. Population distribution of microorganisms in the water body of West Lake,Hangzhou.Acta Ecol Sin(生态学报),19(3):435～440 (in Chinese)
[16] Wu G-F(吴根福),Wu X-C(吴雪昌),Wu J(吴洁),et al 2000. Studies on ecological distribution of microorganisms in the West Lake,Hangzhou.Acta Hydrobiol Sin(水生生物学报),24(6): 589～596 (in Chinese)