微小原甲藻的生长及其对锌限制的响应

应用生态学报 ›› 2003, Vol. ›› Issue (7): 1140-1142.

微小原甲藻的生长及其对锌限制的响应

胡晗华^1,2, 石岩峻^1,3, 丛威¹, 蔡昭铃¹, 欧阳藩¹

1. 中国科学院过程工程研究所生化工程国家重点实验室, 北京 100080;
2. 厦门大学生命科学学院, 厦门 361005;
3. 北京化工大学化学工程学院, 北京 100029

收稿日期:2002-12-18 修回日期:2003-04-12 出版日期:2003-07-15
通讯作者: 胡晗华,男,1971年11月生,博士,主要从事藻类生物学研究,发表论文10余篇.E-mail:hanhuahu@sohu.com.
基金资助:
国家重点基础研究发展规划项目(2001CB409706);国家自然科学基金资助项目(20176060).

Response of Prorocentrum minimum growth to zinc limitation

HU Hanhua^1,2, SHI Yanjun^1,3, CONG Wei¹, CAI Zhaoling¹, OUYANG Fan ¹

1. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China;
2. School of Life Sciences, Xiamen University, Xiamen 361005, China;
3. School of Chemical Engineering, Beijing University of Chemical Technology, Beijing 100029, China

Received:2002-12-18 Revised:2003-04-12 Online:2003-07-15

摘要/Abstract

摘要： 研究了低中高3种Zn²⁺浓度下,赤潮藻微小原甲藻的生长和生理响应.结果表明,低Zn(1.4pmol·L^-1)下,藻细胞的比生长速率和稳定期生物量分别为0.40d^-1和51100cell·ml^-1.当Zn²⁺浓度超过24.4pmol·L^-1时,提高Zn²⁺浓度(181.6pmol·L^-1),藻细胞的比生长速率没有改变,为0.93d^-1,而稳定期生物量则略有下降,但均明显高于低Zn条件下藻细胞的比生长速率和稳定期生物量.Zn限制条件下藻细胞的叶绿素a合成受到影响,藻细胞光合作用需在更高光强下达到饱和.随着Zn²⁺浓度增加藻细胞光饱和的光合作用速率(Pm)及光合作用效率(α)均明显增大.研究表明,富营养化水体中,高的Zn浓度是一定条件下触发赤潮藻类爆发性增殖的重要因子之一.

Abstract: Studies on the growth and physiological response of red tide alga Prorocentrum minimum to three Zn ²⁺ levels were showed that the specific growth rate and biomass were limited in low Zn ²⁺-grown cells (1.4 pmol·L^-1, which were 0.40 d^-1 and 51100 cell·ml^-1 respectively. The specific growth rate was not significantly different when the Zn ²⁺ concentration in medium was increased over 24.4 pmol·L^-1,but there was a slight decrease in biomass; however, both specific growth rate and biomass were much higher than those in low Zn ²⁺-grown cells. It was also showed that chlorophyll a synthesis was limited due to Zn ²⁺ deficient,and therefore,the cells became light saturated at higher irradiance under Zn-limited condition. Light-saturated photosynthetic rates (Pm) and photosynthetic efficiency (α) increased significantly with increasing Zn ²⁺ concentrations. It was concluded that Zn ²⁺ concentration might be one of the key factors affecting red tide blooms in eutrophication environment.

中图分类号:

Q178.53

胡晗华, 石岩峻, 丛威, 蔡昭铃, 欧阳藩. 微小原甲藻的生长及其对锌限制的响应[J]. 应用生态学报, 2003, (7): 1140-1142.

HU Hanhua, SHI Yanjun, CONG Wei, CAI Zhaoling, OUYANG Fan . Response of Prorocentrum minimum growth to zinc limitation[J]. Chinese Journal of Applied Ecology, 2003, (7): 1140-1142.

参考文献

[1] Boyer GL and Brand LE. 1998. Trace elements and harmful algal blooms. In: Anderson DM,Cembella AD,Hallegraeff GM eds. Physiological Ecology of Harmful Algal Blooms. Berlin:Springer Verlag. 490～502
[2] Brand LE,Sunda WG,Guillard RRL. 1983. Limitation of marine phytoplankton reproductive rates by zinc,manganese and iron.Limnol Oceanogr,28:1182～1198
[3] Bruland KW,Donat JR,Hutchins DA. 1991. Interactive influences of bioactive trace metals on biological production in oceanic waters.Limnol Oceanogr,36:1555～1577
[4] Buitenhuis ET. Interactions between Emiliania huxleyi and the dissolved inorganic carbon system. Doctoral Dissertation. Netherlands,Netherlands Institute for Sea Research. 42～43
[5] Coale KH. 1991. Effects of iron,manganese,copper and zinc enrichments on productivity and biomass in the sub-arctic Pacific Ocean.Limnol Oceanogr,36:1851～1864
[6] Harrison PJ,Waters RE,Taylor FJR. 1980. A broad spectrum artificial seawater medium for coastal and open ocean phytoplankton.J Phycol,16:28～35
[7] Henley WJ. 1993. Measurement and interpretation of photosynthetic light-response curves in alga in the context of photoinhibition and diel changes.J Phycol,29:729～729
[8] Hodgkiss IJ and Ho KC. 1997. Are changes in N:P ratios in coastal waters the key to increased red tide blooms? Hydrobiologia,352:141～147
[9] Huang B-Q(黄邦钦),Xu P(徐鹏),Hu H-Z(胡海忠),et al. 2000. Effects of Fe and Mn on growth and cell size of Alexandrium tamarense under different culture conditions.Acta Sci Circums(环境科学学报),20(5): 537～541(in Chinese)
[10] Hwang DF and Lu YH. 2000. Influence of environmental and nutritional factors on growth,toxicity,and toxin profile of dinoflagellate Alexandrium minutum. Toxicon,38(11):1491～1503
[11] Jeffrey SW and Humphrey GF. 1975. New spectrophotometric equations for determining chlorophylls a,b,c1 and c2 in higher plants,algae and natural phytoplankton.Biochem Physiol Pflanz,167:191～194
[12] Lane TW and Morel FMM. 2000. Regulation of carbonic anhydrase expression by zinc,cobalt,and carbon dioxide in the marine diatom Thalassiosira weissflogii. Plant Physiol,123:345～352
[13] Li D-Y(李德耀),Ye J-Y(叶济宇). 1986. Some technical problems in using oxygen electrode.Plant Physiol Commun(植物生理学通讯),5:56～58(in Chinese)
[14] Lin Y(林昱),Tang S-M(唐森铭),Chen X-L(陈孝麟),et al.1994. Impact of dissolvable Fe on multiplication of red tide diatom in enclosed water column.Mar Sci Bull(海洋通报),13(5): 14～18(in Chinese)
[15] Liu D-Y(刘东艳),Sun J(孙军),Chen Z-T(陈宗涛),et al.2002. Effect of N/P ratio on the growth of a red tide diatom Skeletonema costatum. Trans Oceanol Limnol(海洋湖沼通报),(2): 39～44(in Chinese)
[16] Morel FMM,Reinfelder JR,Roberts SB,et al.1994. Zinc and carbon co-limitation of marine phytoplankton.Nature,369:740～742
[17] Price NM,Ahner BA,Morel FMM. 1994. The equatorial Pacific Ocean: Grazer-controlled phytoplankton populations in an iron-limited ecosystem.Limnol Oceanogr,39:520～534
[18] Siu GKY,Young MLC,Chan DKO. 1997. Environmental and nutritional factors which regulate population dynamics and toxin production in the dinoflagellate Alexandrium catenella. Hydrobiologia,352:117～140
[19] Sunda WG. 1989. Trace metal interactions with marine phytoplankton.Biol Oceanogr,6:411～ 442
[20] Zhou M-J(周名江),Zhu M-Y(朱明远),Zhang J(张经).2001. State of harmful algal blooms and related research activities in China.Chin Bull Life Sci(生命科学),13(2): 54～59(in Chinese)