短期菲胁迫对大豆幼苗超氧化物歧化酶活性及丙二醛含量的影响

应用生态学报 ›› 2003, Vol. ›› Issue (4): 581-584.

短期菲胁迫对大豆幼苗超氧化物歧化酶活性及丙二醛含量的影响

刘宛, 李培军, 周启星, 孙铁珩, 台培东, 许华夏, 张海荣

中国科学院沈阳应用生态研究所, 沈阳 110016

收稿日期:2002-09-20 修回日期:2002-11-18 出版日期:2003-04-15
通讯作者: 刘宛,女,1963年生,博士,副研究员,主要从事污染生态学研究,发表论文20余篇,E-mail:liuwan63@hotmail.com.
基金资助:
中国科学院知识创新工程项目(KZCX2-401,KZCX2-SW-416,SCXZD0103);国家重点基础研究发展规划项目(G19990118);中国科学院沈阳生态实验站(K88GGSBS0202);中国科学院陆地生态过程重点实验室基金资助项目.

Effect of short-term phenanthrene stress on SOD activities and MDA contents in soybean (Glycine max) seedlings

LIU Wan, LI Peijun, ZHOU Qixing, SUN Tieheng, TAI Peidong, XU Huaxia, ZHANG Hairong

Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China

Received:2002-09-20 Revised:2002-11-18 Online:2003-04-15

摘要/Abstract

摘要： 研究了不同浓度(0～200μg·g^-1)菲胁迫和恢复培养后大豆幼苗生长、超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量的变化.结果表明,200μg·g^-1菲处理5d后大豆幼苗生长受到抑制,但幼苗恢复培养后经短暂停滞期后仍可恢复生长.菲污染对大豆幼苗SOD活性变化的剂量效应关系的作用形式比较复杂,胁迫2d时为线性关系,胁迫5d和8d时为抛物线型.在菲处理前期(2d),幼苗SOD活性被100和200μg·g^-1菲显著诱导[分别为对照的1.15倍(P<0.05)和1.26倍(P<0.01)].菲暴露8d时,SOD活性显著降低,200μg·g^-1菲处理组SOD活性为对照的88%(P<0.05).菲处理5d后恢复培养2d和4d,50和100μg·g^-1菲处理组幼苗SOD活性得到恢复,而200μg·g^-1菲处理组幼苗SOD活性仍明显高于对照(P<0.05).试验亦反映出,100和200μg·g^-1菲处理5d和8d,幼苗MDA含量均比对照显著增加(P<0.05和P<0.01).可以认为,SOD活性可作为大豆幼苗遭受短期菲胁迫的生物标记物.

Abstract: Growth of soybean (Glycine max) seedlings, changes of superoxide dismutase (SOD) activities, and malonyldialdehyde (MDA) contents were researched during different concentration phenanthrene stress and following recovery from stress. The results showed that seedling growth was interrupted after 5d in 200μg·g^-1 phenanthrene treatment. However, during the period of recovery, growth was restored, although there was a delay in returning to control levels.The dose-related response of SODactivities were observed in manner of linear for 2d and of parabola for 5d and 8d at 50～200μg·g^-1 phenanthrene. SODactivities of seedling were significantly induced by 100 and 200μg·g^-1 phenanthrene for 2d (P<0.05 and P<0.01), while declined significantly under phenanthrene stress for 8d, and was 88% of control at 200μg·g^-1 phenanthrene (P<0.05). When releasing soybean seedlings from phenanthrene stress for 5d to clear tap water for 2d or 4d,their SODactivities at 50 and 100μg·g^-1 phenanthrene recovered towards control level, while a significant increase in SOD activities was observed at 200μg·g^-1 phenanthrene (P<0.05). The experiments also revealed that a significant increase of MDA contents in seedlings occurred after 5d and 8d in100 and 200μg·g^-1 phenanthrene treatment (P<0.05 and P<0.01), which suggested that the membrane systems were damaged by phenanthrene stress. All of the above results showed that SOD activities of soybean seedling might be suitable to be the biomarkers of phenanthrene stress.

中图分类号:

X173

刘宛, 李培军, 周启星, 孙铁珩, 台培东, 许华夏, 张海荣. 短期菲胁迫对大豆幼苗超氧化物歧化酶活性及丙二醛含量的影响[J]. 应用生态学报, 2003, (4): 581-584.

LIU Wan, LI Peijun, ZHOU Qixing, SUN Tieheng, TAI Peidong, XU Huaxia, ZHANG Hairong . Effect of short-term phenanthrene stress on SOD activities and MDA contents in soybean (Glycine max) seedlings[J]. Chinese Journal of Applied Ecology, 2003, (4): 581-584.

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