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应用生态学报 ›› 2002, Vol. ›› Issue (8): 957-961.

• 研究论文 • 上一篇    下一篇

紫花苜蓿种子对逆境贮藏条件的反应

李春杰, 王彦荣, 朱廷恒, 余玲   

  1. 兰州大学草地农业科技学院, 甘肃草原生态研究所, 兰州 730020
  • 收稿日期:2002-04-07 修回日期:2002-05-09 出版日期:2002-08-15
  • 通讯作者: 李春杰,男,1968年生,在读博士,副研究员,主要从事牧草与草坪草病害的研究,发表论文18篇.E-mail:licj68@public.lz.gs.cn
  • 基金资助:
    国家重点基础研究发展规划项目(G2000018602);国家自然科学基金资助项目(39770537).

Response of alfalfa seed to stress storage conditions

LI Chunjie, WANG Yanrong, ZHU Tingheng, YU Ling   

  1. College of Pastoral Agriculture Science and Technology, Lanzhou University, Gansu Grassland Ecological Research Institute, Lanzhou 730020
  • Received:2002-04-07 Revised:2002-05-09 Online:2002-08-15

摘要: 以陇东紫花苜蓿(Medicago sativaL.cv.“Longdong”)种子为材料,在室温、35℃和35℃+10%的种子含水量(SMC)3种贮藏、接种或不接种燕麦镰刀菌(Fusarium avenaceum(Fr.)Sacc.)的条件下,1年贮藏期内对各逆境处理的种子每隔60d进行1次标准发芽试验,20℃恒温、第10d统计种子的发芽率和死亡率,试验结束时计测种子幼苗的长度和感病率;在大田条件下观测各处理种子的出苗率,确定催腐(CD)与各种贮藏条件下的苜蓿种带真菌种类和检出率.结果表明,随着贮藏温度和种子含水量等逆境贮藏条件胁迫的加剧,苜蓿种带真菌检出率逐渐增高,从室温、35℃条件下的10%上升到CD+35℃+10%SMC条件下的29%;抗病性逐渐减弱,35℃+10%SMC条件下幼苗的感病率和种子死亡率显著(P<0.05)高于室温和35℃下的感病率和种子死亡率;室内种子发芽率和田间出苗率逐渐下降,35℃+10%SMC条件下的种子的发芽率和田间出苗率显著(P<0.05)低于在室温和35℃下的发芽率和田间出苗率;幼苗生长受到抑制,35℃+10%SMC条件下的苗长和根长显著(P<0.05)低于在室温和35℃下的幼苗长度.随着贮藏时间的延长,种子真菌检出率和田间出苗率下降,幼苗感病率增加.与未接种的对照相比,接种燕麦镰刀菌的种子.

Abstract: The seed germination rate, seed mortality, seedling length, and infection rate of alfalfa (Medicago sativa L.cv.'Longdong') were measured at constant temperature 20℃ every 60 days during one year storage period after inoculated or no inoculated by Fusarium avenaceum under room temperature (RT),35℃,and 35℃ and +10% seed moisture content (SMC) conditions. Field emergence rates of seeds under above treatments were also observed,and seed borne fungi were detected under the conditions mentioned above and controlled deterioration (CD) as well. The results showed that the percentage of isolated alfalfa seed borne fungi was increased from 10% under room temperature and 35℃ to 29% under 35℃+10%SMC.Disease resistance was declined, and seed mortality and seedling infection rate under 35℃+10%SMCwere significantly higher than those under room temperature and 35℃ respectively (P<0.05). The seed germination rate and field emergence rate were also decreased significantly (P<0.05). Seedling shoot and root length under 35℃+10%SMCwere significantly less than those under RTand 35℃ respectively (P<0.05).The percentages of both seed borne fungi isolated and field emergence were decreased,and that of seedling infection was increased with storage period extending from 60 to 360 days. Compared to no inoculated control,the percentage of seed germination, seedling shoot and root length were decreased, and seed mortality and seedling infection rate were increased after inoculated by F.avenaceum.

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