铝毒胁迫诱导菜豆柠檬酸的分泌与累积

应用生态学报 ›› 2002, Vol. ›› Issue (3): 307-310.

铝毒胁迫诱导菜豆柠檬酸的分泌与累积

沈宏, 严小龙, 郑少玲, 王秀荣

华南农业大学植物营养遗传实验室和根系生物学研究中心, 广州 510642

收稿日期:2000-06-12 修回日期:2001-09-18 出版日期:2002-03-15
通讯作者: 沈宏,男,1970年生,博士,副教授,主要从事植物根际营养与环境胁迫方面的研究,发表论文20篇.Tel:020-85281822,E-mail:shenong@163.net
基金资助:
国家自然科学基金(39970431、39925025);广东省自然科学基金(000642);国际基金(C/30421)资助项目

Exudation and accumulation of citric acid in common bean in response to Al toxicity stress

SHEN Hong, YAN Xiaolong, ZHENG Shaoling, WANG Xiurong

South China Agricultural University, Guangzhou 510642

Received:2000-06-12 Revised:2001-09-18 Online:2002-03-15

摘要/Abstract

摘要： 水培试验结果表明,铝毒诱导菜豆柠檬酸的分泌与累积存在着显著的基因型差异.Al³⁺浓度<50μmol·L^-1时,柠檬酸分泌量随Al³⁺浓度的增大而增加;Al³⁺浓度在50～80μmol·L^-1时,柠檬酸分泌量随Al³⁺浓度的增大而减小.不同菜豆基因型以G19842的柠檬酸分泌量最大,单位干重Al吸收量最小.铝毒胁迫时,不同菜豆基因型叶片柠檬酸累积量无明显差异,根系柠檬酸累积量为G19842>AFR>ZPV>G5273.菜豆柠檬酸分泌量缺P处理<铝毒胁迫,50μmol·L^-1LaCl3不能诱导菜豆分泌柠檬酸,表明柠檬酸的分泌与累积是菜豆抗铝毒胁迫的重要生理反应.

Abstract: Significant differences in the exudation and accumulation of citric acid in common bean genotypes were observed in response to Al toxicity stress by hydroponic cultural experiments. Secreted citric acid increased with increasing external concentrations of Al³⁺ which ranged from 0 to 50 μmol·L^-1,while ranged from 50 to 80 μmol·L^-1,secreted citric acid decreased with increasing external concentrations of Al³⁺. Among different genotypic common beans,citric acid secreted in G19842 was the largest,while Al uptake per unit dry weight in G19842 was the least. No difference in the accumulation of citric acid in leaves was found among different genotypic common beans,while the size of the content of citric acid in roots was G19842>AFR>ZPV>G5273. The amount of citric acid exuded was smaller induced by phosphorus deficiency than that induced by Al toxicity stress. Exposure to 50 μmol·L^-1 LaCl 3 could not induce the exudation of citric acid,and it implied that the exudation and accumulation of citric acid in common bean was an important physiological response of resistance to Al toxicity stress.

中图分类号:

Q945

沈宏, 严小龙, 郑少玲, 王秀荣. 铝毒胁迫诱导菜豆柠檬酸的分泌与累积[J]. 应用生态学报, 2002, (3): 307-310.

SHEN Hong, YAN Xiaolong, ZHENG Shaoling, WANG Xiurong . Exudation and accumulation of citric acid in common bean in response to Al toxicity stress[J]. Chinese Journal of Applied Ecology, 2002, (3): 307-310.

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