土壤细菌16SrRNA基因变异型及其与植被的相关研究

应用生态学报 ›› 2001, Vol. ›› Issue (5): 757-760.

土壤细菌16SrRNA基因变异型及其与植被的相关研究

杨官品, 朱艳红, 陈亮, 薛小乔

湖北大学生命科学学院, 武汉 430062

收稿日期:1999-10-14 修回日期:2000-01-14 出版日期:2001-09-25
通讯作者: 杨官品,男,1963年生,博士,教授.现主要从事分子生态学研究.现已发表论文30余篇.
基金资助:
湖北省自然科学基金和武汉市晨光计划资助项目.

Molecular genetic variation of soil bacteria as determined by polymorphism of restrictive fragment length of 16SrRNA genes and its relation w ith vegetation shift

YANG Guanpin, ZHU Yanhong, CHEN Liang, XUE Xiaoqiao

Faculty of Life Science, Hubei University, Wuhan 430062

Received:1999-10-14 Revised:2000-01-14 Online:2001-09-25

摘要/Abstract

摘要： 绕过细菌的分离培养,直接提取土壤DNA,扩增、克隆土壤细菌群体的16S核糖体RNA基因(16SrD-NA).根据该基因各种变异类型的限制性片段长度多型性,分析土壤细菌分子遗传多样性及其与植被的相互关系.植被的改变影响土壤养分,进而改变土壤细菌群落结构.土壤细菌遗传多样性和分化能反映植被的变化.

中图分类号:

S154.4

杨官品, 朱艳红, 陈亮, 薛小乔. 土壤细菌16SrRNA基因变异型及其与植被的相关研究[J]. 应用生态学报, 2001, (5): 757-760.

YANG Guanpin, ZHU Yanhong, CHEN Liang, XUE Xiaoqiao . Molecular genetic variation of soil bacteria as determined by polymorphism of restrictive fragment length of 16SrRNA genes and its relation w ith vegetation shift[J]. Chinese Journal of Applied Ecology, 2001, (5): 757-760.

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