鹤山人工林大叶相思种群遗传结构的AFLP分析

应用生态学报 ›› 2001, Vol. ›› Issue (4): 491-495.

鹤山人工林大叶相思种群遗传结构的AFLP分析

张军丽, 王峥峰, 王伯荪, 李鸣光, 张炜银

中山大学生命科学学院, 广州 510275

收稿日期:2000-09-20 修回日期:2000-12-18 出版日期:2001-07-25
通讯作者: 张军丽,女,1954年生,博士,副教授,主要从事植物分子生态学研究,已发表论文30多篇.E-mail:ls31@zsu.edu.cn
基金资助:
国家自然科学基金重大项目(39899370);国家自然科学基金(39670136);国家教育部博士点基金(98055808);中国科学院鹤山丘陵综合开放试验站基金资助项目

AFLP analysis on genetic structure of planted Acacia auriculiformis population in Heshan

ZHANG Junli, WANG Zhengfeng, WANG Bosun, LI Mingguang, ZHANG Weiyin

School of Life Sciences, Zhongshan University, Guangzhou 510275

Received:2000-09-20 Revised:2000-12-18 Online:2001-07-25

摘要/Abstract

摘要： AFLP分析种源相同、栽种时间相同的不同群落中的大叶相思种群.结果表明,其杂合度在纯林中最高,豆科混交林次之,非豆科混交林中最低.种群遗传多样性主要分布在种群内,达90.73%,不同种群间仅为9.27%.纯林与豆科混交林的大叶相思两种群间遗传结构关系较远,纯林与非豆科混交林种群间遗传关系较近,豆科混交林与非豆科混交林种群间遗传关系居中.表明人工林大叶相思种群总的遗传变化水平较高,但其遗传分化并非基因流作用所致,而是受人工选育与环境变化的影响.

Abstract: AFLP analysis of Acacia auriculiformis populations in 3 different planted communities with the same provenance and same planting time indicated that the genetic heterozygosity was quite high in the plantation population.90.73% of total genetic variance distributed within a population,while only 9.27% among populations.The most distinct genetic structural difference was found between pure plantation and legume mixed plantation,next was between legume and non-legume mixed plantation,and the most similar was between pure and non-legume mixed plantation,which revealed that the genetic variation in this Acacia auriculiformis population was relatively higher than that from the natural forests by isoenzymic analysis data.The genetic differentiation was not the result of gene flow,but the result of the artificial selection and the microenvironmental changes.

中图分类号:

Q145

张军丽, 王峥峰, 王伯荪, 李鸣光, 张炜银. 鹤山人工林大叶相思种群遗传结构的AFLP分析[J]. 应用生态学报, 2001, (4): 491-495.

ZHANG Junli, WANG Zhengfeng, WANG Bosun, LI Mingguang, ZHANG Weiyin . AFLP analysis on genetic structure of planted Acacia auriculiformis population in Heshan[J]. Chinese Journal of Applied Ecology, 2001, (4): 491-495.

参考文献

[1] Barrett BA, Kidwell KK. 1998. AFLP-based genetic diversity assessment among wheat cultivars from the Pacific Northwest. Crop Sci, 38:1261～1271
[2] Doyle JJ, Doyle JL. 1987. A rapid DNA isolation procedure for smallquantities of fresh leaf tissue. Phytochem Bull, 19:11～15
[3] Excoffler L, Smouse PE, Quattro JM. 1992. Analysis of molecular variance inferred from metric distances among DNA haplotypes:applicationto human mitochondrial DNA restriction data. Genetics, 131:479～491
[4] Felsenstein J. 1993. PHYLIP(Phylogeny Inference Package)version3.5c. St Louis:University of Washington.
[5] Lerceteau E, Szmidt AE. 1998. QTL mapping wood production andwood quality traits in Pinus sylvestris L. using AFLP markers. Eighteenth Interna Cong Genetics, 176(8):240
[6] Lewontine RC. 1973. The apportionment of human diversity. Ecol Biol,6:381～398
[7] Li WH, Luo CC. 1985. A new method for estimation synonymous andnonsynonymous rates of nucleotide substitution considering the relativelikelihood of nycleotide and codon changes. Mol Biol Evol, 2:150～174
[8] Li Z-A(李志安),Weng H(翁轰),Yu Z-Y(余作岳).1995. Plants-tion effects on mineralization of soil nitrogen. Chin Bot Bull(植物学通报),12:142～148(in Chinese)
[9] Moran GF, Muora O, Bell JC. 1989. Breeding systems and genetic diversity in Acacia auriculiformis and A. crassicapa. Biotropica, 21(3):250～256
[10] Mouna O, Moran GF, Bell JC. 1991. Hierarchical patterns of correlatedmating in Acacia melanaxylon. Genetics, 127:619～629
[11] Muluvi GM, Sprent JI, Soranzo N et al. 1999. Amplified fragmentlength polymorphism(AFLP)analysis of genetic variation in Moringaoleifera Lam. Mol Ecol, 8(3):463～470
[12] Nei M. 1987. Molecular Evolutionary Genetics. New York:ColumbiaUniversity Press.
[13] Peng S-L(彭少麟),Xiang Y-C(向言词).1999. The invasion of exoticplants and effects of ecosystems. Acta Ecol Sin(生态学报),19(4):560～569(in Chinese)
[14] Powell W, Thomas WTB, Baird E et al. 1997. Analysis of quantitativetraits in barley by the use of amplified fragment length polymorphism.Heredity, 79:48～59
[15] Ren H(任海),Peng S-L(彭少麟).1998.The ecological and biolog-ical characteristics of Acacia auriculae formis. Guihaia(广西植物),18(2):146～152(in Chinese)
[16] Rohlf FJ. 1993. NTSYS-pc:Numerical Taxonomy and MultivariatsAnalysis System, Version 1. 8. Applied Biostatistics. New Youk:Steauket.
[17] Vos P, Hogers R, Bleeker M et al. 1995. AFLP:a new technique forDNA fingerprinting. Nucleic Acids Rts, 23:4407～4414
[18] Wang Z-F(王峥峰),Zhang J-L(张军丽),Wang B-S(王伯荪)2000. Molecular ecology of Cryptocarya ohinensis populations in different communities. Chin J Appl Ecol(应用生态学报),11(3):342～344(in Chinese)
[19] Weng Q-J(翁启杰),Zheng H-S(郑海水),Yang Z-J(杨曾奖)1997. Research on provenance trial of Acacia auriculiformis. Guangdong For Sci Tech(广东林业科技),13(2):12～16(in Chinese)
[20] Yu Z-Y(余作岳),Peng S-L(彭少麟),Zhang W-Q(张文其).1990.Coenological analysis of the man-made forest on down land in Heshan,Guangdong Province III. Acacia auriculaeformis forest. Trop Subtrop For Ecosys(热带亚热带森林生态系统研究),7:163～168(inChinese)
[21] Zeng Y-T(曾玉田),Yang M-Q(杨民权).1990. Provenance test ofAcacia auriculi formis. Guangdong For Sci Tech(广东林业科技),6(5):25～29(in Chinese)
[22] Zhong L(钟翎),Qu L-H(屈良鹄).1999. Recent research advancesin plant small nucleolar RNA. Acta Bot Sin(植物学报),41(6):563～569(in Chinese)