盐逆境下转基因耐盐小麦与其受体呼吸途径的动态变化

应用生态学报 ›› 2000, Vol. ›› Issue (6): 873-877.

盐逆境下转基因耐盐小麦与其受体呼吸途径的动态变化

孔英珍, 周功克, 崔凯荣, 王亚馥, 李红玉

兰州大学干旱农业生态国家重点实验室, 兰州730000

收稿日期:1999-10-29 修回日期:2000-01-07 出版日期:2000-12-25
通讯作者: 王亚馥
基金资助:
甘肃省“97”重点农业科技攻关项目(GK971222A1)

Dynamics of respiration pathway in transgenic salt-tolerant wheat and its receptor under salt stress

KONG Yingzhen, ZHOU Gongke, CUI Kairong, WANG Yafu, LI Hongyu

State Key Laboratory of Arid Agroecology, Lanzhou University, Lanzhou 730000

Received:1999-10-29 Revised:2000-01-07 Online:2000-12-25

摘要/Abstract

摘要： 以转基因耐盐品种89122和其受体陇春13号两种小麦为实验材料,研究了小麦幼苗在不同盐浓度胁迫下呼吸途径动态变化.结果表明,89122出现盐呼吸明显迟于其受体;两品种的V_alt与ρV_alt变化并不同步,且V_alt均受高盐浓度的抑制,但低盐浓度能诱导其受体的V_alt;两品种的ρV_alt与ρ'V_cyt彼此协同调节适应盐逆境,且ρ'V_cyt仍是盐胁迫过程中线粒体电子传递的主要途径.同时讨论了盐逆境下抗氰呼吸的一些可能生理作用,提出抗氰呼吸运行的强弱作为筛选植物耐盐性的一个生理指标是可行的.

Abstract: Using transgenic salt-tolerant wheat 89122 and its receptor Longchun 13 as test material, the dynamic changes of respiration pathway of wheat seedlings at different salt concentration were studied.The appearance of salt respiration was later in 89122 than in Longchun 13.The changes of V_alt and ρV_alt of both varieties had different patterns during salt stress process.V_alt was suppressed by high salt levels, but could be induced by low salt levels in Longchun 13. The ρV_alt and ρ'V_cyt of two varieties were cooperatively regulated with each other to adapt to salt stress,and ρ'V_cyt was the main electron transport pathway.The possible physiological roles of cyanide-resistant respiration were also discussed.It was suggested that the operation intensity of cyanide-resistant respiration could serve as a physiological index of plant salt-tolerance.

孔英珍, 周功克, 崔凯荣, 王亚馥, 李红玉. 盐逆境下转基因耐盐小麦与其受体呼吸途径的动态变化[J]. 应用生态学报, 2000, (6): 873-877.

KONG Yingzhen, ZHOU Gongke, CUI Kairong, WANG Yafu, LI Hongyu. Dynamics of respiration pathway in transgenic salt-tolerant wheat and its receptor under salt stress[J]. Chinese Journal of Applied Ecology, 2000, (6): 873-877.

参考文献

[1] Bahr JT, Bonner WD. 1973. Cyanide-insensitive respiration I. The study states of skunk cabbage spadix and bean hypocotyl mitochondria. J Biol Chem,248:3441～3445 [2] Douce R, Neuburger M. 1989. The uniqueness of plant mitochondria. Ann Rev Plant Physiol Plant Mol Biol,40:371～414 [3] He J-X(何军贤),Wei Zh-Q(韦振泉),Liang H-G(梁厚果).1999. Effects of water stress on development, operation and gene expression of cyanide-resistant respiratory pathway in wheat. Sci China(中国科学)(Series C),42(3):300～306(in Chinese) [4] Kinner CM, Beramlage WJ, 1981. Temperature effects on the activity of the alternative respiratory pathway in chill-sensitive Cucumis sativus. Plant Physiol,68:1474～1478 [5] Liu Y-L(刘友良),Wang L-G(汪良).1998. The adaptability of plant to salt stress. In: Yu S-W(余叔文),Tang Z-C(汤章城)eds. Plant Physiology and Molecular Biology (2nd edition). Beijing:Science Press.721～738(in Chinese) [6] Maxwell DP, Wang Y, Mclntosh L. 1999. The alternative oxidase lowers mitochondrial reactive oxygen production in plant cells. Proc Natl Sci USA,96(14):8271～8276 [7] Mclntosh L. 1994. Molecular biology of the alternative oxidase. Plant Physiol,105:781～786 [8] Ni J-F(倪建福),Zhon W-L(周文麟),Wang Y-F(王亚馥).1995.A new wheat line obtained by introducing sorghum DNA. Proceedings of the Eighth International Wheat Genetics Symposium. Vol I,30～35(in Chinese) [9] Jollvet Y, Pireaux JC, Dizengremel P. 1990. Changes in properties of barley leaf mitochondria isolated from NaCl-treated plants. Plant Physiol,94:641～646 [10] Popov VN, Simonian RA, Skulachev VP. 1997. Inhibition of the alternative oxidase stimulates H₂O₂ production in plant mitochondria. FEBS Letters,415:87～90 [11] Puris AC. 1997. Role of the alternative oxidase in limiting superoxide production by plant mitochondria. Physiol Plant,100:165～170 [12] Qin L(秦琳),Cui K-R(崔凯荣),Wang Y-F(王亚馥).1997. RAPD verification of rust resistant wheat variants. Acta Bot Boreall-Occident Sin(西北植物学报),17(6):22～26(in Chinese) [13] Stewart GR, Larher F. 1982. Accumulation of amino acids and related compounds in relation to environmental stress. In: Miflin BJ ed. Biohemistry of Plants. New York: Academic Press. 609～635 [14] Tang X-X(唐学玺),Jia J-F(贾敬芬),Zheng G-C(郑国昌).1999. Damage responses to salt stress in salt-tolerant cell line of wheat. Acta Bot Sin(植物学报),41(7):575～760(in Chinese) [15] Theologis A, Laties GG. 1978. Relative contribution of cytochromemadiated and cyanide-resistant electron transport in fresh and aged potato slices. Plant Physiol,62:232～237 [16] Umbach H, Siedovo JN. 1993. Covalent and noncovalent dimers of the cyanide-resistant alternative oxidase protein in higher plant mitochondria and their relationship to enzyme activity. Plant Physiol, 103:845～854 [17] Vanlerberghe GC, Day DA, Wiskich JT. 1995. Alternative oxidase activity in tobacco leaf mitochondria: dependence on tricarboxylic acid cycle-mediated redox regulation and pyruvate activation. Plant Physiol,109.353～361 [18] Vanlerberghe GC. 1997. Alternative oxidase: From gene to function. Ann Rev Plant Physiol Plant Mol Biol,48:703～734 [19] Vanlerberghe CC, Mclntosh L. 1992. Lower growth temperature increases the alternative pathway capacity and AOX protein in tobacco. Plant Ph ysiol,100:115～119 [20] Wang Y-F(王亚馥),Cui K-R(崔凯荣),Chen K-M(陈克明).1993. Ultrastructural studies of plastids of albino mutant of wheat introduced exogenous DNA of corn. In: ZhouG-Y(周光字),Chen S-B(陈善葆),Huang J-Q(黄骏麒)eds. Advances in Molecular Breeding Research of Agriculture. Beijing: China Agricultural Technology Press. 86～91 (in Chinese) [21] Wang Y-F(王亚馥),Chen K-M(陈克明),liao C-J(焦陈谨).1995. Studies on biological characters and endosperm protein of variation lines obtained by introducing exogenous DNA into wheat. Acta Agron Sin(作物学报),2l(4):404～411(in Chinese) [22] Wang Y-F(王亚馥),Chen K-M(陈克明),Wang X-Z(王晓哲).1995. Two-dimensional gel electrophoresis study on endosperm protein of the new line obtained by introducing DNA of sorghum into wheat. Acta Bot Sin(植物学报),7(1):2～8(in Chinese) [23] Wang X-J(王晓娟),Qin X-C(覃新程),Wang Y-F(王亚馥).1999.Analyses of enzymic antioxidant defenses in new wheat line 89122 under salt stress. J Lanzhou Univ (Natural Sciences)(兰州大学学报),35(1):140～144(in Chinese) [24] Xu Y-L(徐云岭),Yu S-W(余叔文).1990. Energy consumption in adaptability to salt stress plants. Plant Physiol Commun(植物生理学通讯),6:70～73(in Chinese) [25] Zhou W-L(周文麟),Ni J-F(倪建福),Wang Y-F(王亚馥).1992. Studies on DNA transfer from C4 crop to wheat. Acta Agron Sin(作物学报),18(6):418～424(in Chinese)