抗旱性不同的小麦幼苗对水分和NaCl胁迫的反应

应用生态学报 ›› 2000, Vol. ›› Issue (5): 718-722.

抗旱性不同的小麦幼苗对水分和NaCl胁迫的反应

任红旭, 陈雄, 孙国钧, 王亚馥

兰州大学干旱农业生态国家重点实验室, 兰州730000

收稿日期:1999-10-29 修回日期:2000-04-04 出版日期:2000-09-25
通讯作者: 王亚馥
基金资助:
甘肃省科委“九五”攻关项目;中国科学院重点项目(B类)(KZ952-S1-216);中国科学院海北生态定位站基金资助项目

Response of wheat seedlings with different drought resistance to water deficiency and NaCl stresses

REN Hongxu, CHEN Xiong, SUN Guojun, WANG Yafu

State Key Laboratory of Arid Agroecology, Lanzhou Univeristy, Lanzhou 730000

Received:1999-10-29 Revised:2000-04-04 Online:2000-09-25

摘要/Abstract

摘要： 分别测定抗旱小麦8139(Triticum aestivum L.cv.8139)和干旱敏感品种甘麦8号(T.aestivum L.cv.GanmaiNo.8)在20%PEG6000和1.2%NaCl胁迫下的生长、光合作用、蒸腾作用及抗氧化保护系统的变化.结果表明,抗旱小麦8139对PEG6000有较强的抗性,但对NaCl胁迫的抗性较差.NaCl胁迫下,两种小麦根的生长均受到严重抑制,而在PEG胁迫下,则根的生长不仅未受抑制,反而略有促进.PEG与NaCl胁迫下,两种小麦的净光合速率与水分利用效率在各时期均表现出明显差别,而蒸腾作用则在胁迫后第7天与第14天表现出明显差别.干旱胁迫下,抗旱小麦的膜脂过氧化水平在胁迫后第7天显著低于甘麦8号,而在NaCl胁迫下,各时期二者之间均无显著差别.与此相对应的是,NaCl胁迫下,两小麦品种之间除在GSH含量上表现出显著差异外,果聚糖含量、SOD活性及APX活性在各时期均无显著差别.而干旱胁迫下,抗旱小麦8139的果聚糖含量、GSH含量及SOD、APX活性则分别在不同时期高于甘麦8号,表现出较强的抗氧化能力.

Abstract: The growth,photosynthesis,transpiration and antioxidative defence system of the seedlings of drought-tolerant wheat strain 8139 and drought-sensitive strain Ganmai No.8 at 20% PEG 6000 and 1.2% NaCl stresses were compared.The results showed that strain 8139 had a strong drought resistance,but a weak salt resistance.The root growth of both wheat strains was inhibited significantly under salt stress,but stimulated slightly under drought stress.The net photosynthetic rate and water use efficiency of strain 8139 were significantly different from those of Ganmai No.8 at every stage under both drought and salt stresses, and its transpiration rate was significantly different from that of Ganmai No.8 only at 7th and 14th day after being stressed.The MDA content in strain 8139 after being stressed for 7 days was much lower than that in Ganmai No.8 under drought stress,but there was no significant difference between the two strains under NaCl stress.Correspondingly,there was no significant difference in the fructan content and SOD and APX activities between strain 8139 and Ganmai No.8,but a significant difference in GSH content was found under salt stress.Under drought stress,the contents of fructan and GSH and the activities of SOD and APX in strain 8139 were much higher than those in Ganmai No.8 at different stage,and strain 8139 exhibited a strong antioxidative defence ability.

任红旭, 陈雄, 孙国钧, 王亚馥. 抗旱性不同的小麦幼苗对水分和NaCl胁迫的反应[J]. 应用生态学报, 2000, (5): 718-722.

REN Hongxu, CHEN Xiong, SUN Guojun, WANG Yafu. Response of wheat seedlings with different drought resistance to water deficiency and NaCl stresses[J]. Chinese Journal of Applied Ecology, 2000, (5): 718-722.

参考文献

1 Anderson MD, Prasad TK, Stewart CR. 1995. Changes in isozyme profiles of cata lase, peroxidase, and glutathione reductase during acclimation to chilling in m esocotyls of maize seedlings. Plant Physiol, 109:1247～1257
2 Chen Y-W(陈一舞), Shao G-H(邵桂花), Chang R-Z(常汝镇). 1997. The effect of sa lt stress on superoxide dismutase in various organelles from cotyledon of soybea n seedling. Acta Agron Sin(作物学报),23 (2): 214～219 (in Chinese)
3 Colmer TD, Epstein E, Dvorak J. 1995. Differential solute regulation in leaf blades of various ages in salt-sensitive heat and a salt-tolerant wheat × Lophopyrum elongatum (Host) A.Love Amphiploid. Plant Physiol,[ WTHZ 108: 1715～1724
4 Dhindsa RS, Matowe W. 1981. Drought t olerance in two mosses:correlated with e nzymatic defence against lipid peroxidation. J Exp Bot,32 (126): 79～91
5 Foyer CH, Souriau N, Perret S. 1995. Overexpr ession of glutathion reductase but not glutathion synthetase lead to increases i n antioxidant capacity and resistance to photoinhibition in poplar trees. Pla nt Physiol,109: 1047～1057
6 Guri A. 1983. Variation in glutathione of Phaseolus vulgaris prior to and af ter exposure to ozone. Can J Plant Sci,63 : 733～737
7 Liu H-X(刘鸿先), Zeng S-X(曾韶西), Wang Y-R(王以柔). 1985. The effect of low temperature on superoxide dismutase in various organelles of cucumber seedling cotyledon with different cold tolerance. Acta Phytophysiol Sin (植物生理学报),11: 46～57 (in Chinese)
8 Ma C-C(马成仓). 1998. Hg harm on cell membrane of rape leaf and cell endogeno us protection effect. Chin J Appl Ecol(应用生态学报),9(3): 323～326 (in Chinese)
9 May MJ, Leaver CJ. 1993. Oxidative st imulatio n of glutathione synthesis in Arabidopsis thaliana suspension cultures. P lant Physiol,103: 621～627
10 Nakano Y, Asada K. 1981. Hydrogen peroxide is scavenged by ascorbate specifi c peroxidase in spinach chloroplasts. Plant Cell Physiol,22: 867～880
11 Perez-Altocea F, Larher F. 1995. Effects of phlorizin and p-chloromercuriben zenesulfonic acid on sucrose and proline accumulation in detached tomato leaves submitted to NaCl and osmotic stresses. J Plant Physiol,14 5: 367～373
12 Pilon-Smits EAH, Ebskamp MJM, Paul MJ. 1995. Improved performance of transgenic fructan-accumulating tobacco under drought stress. Plant Physiol,107:125～130
13 Price AH, Hendry GAF. 1989. Stress and the role of activated oxygen scanveng ers and protective enzymes in plants subjected to drought. Bio Soci Trans,17: 483～494
14 Qin X-Q(秦小琼), Jia S-R(贾士荣). 1997. Genetic engineering of plants tolera nt to oxidative stress. J Agric Biotech (农业生物技术学报),4:14～22(in Chinese)
15 Rennenberg H, Polle A. 1994. Protection from oxidative stress in transgenic plant. Bio Soci Trans,22: 936～940
16 Rennenberg H. 1982. Glutathione metabolism and possible biological roles in higher plants. Phytochem,21:2771～2781
17 Wang Y-R(王以柔), Liu H-X(刘鸿先), Li P(李平). 1986. The effect of chilling stress on membrane-lipid peroxidation of photosynthetic apparatus in rice seedl ings in the dark and light. Acta Phytophysiol Sin(植物生理学报),12(3): 244～251(in Chinese)
18 Zeng S-X(曾韶西) Wang Y-R(王以柔) Li M-R(李美如). 1997. Comparison of the changes of membrane protective system in rice seedlings during enhancement of ch illing resistance by different stress pretreatment. Acta Bot Sin (植物学报),39(4): 308～314 (in Chinese)