影响根癌农杆菌转化的因素及其在单子叶作物上的应用

摘要/Abstract

摘要： 在植物转基因方法中,根癌农杆菌介导的遗传转化应用最为广泛,进一步提高其转化频率并扩大其宿主范围到禾谷类作物是人们所关注的问题.有多种因素影响根癌农杆菌的转化频率,包括植物的受伤反应、细菌的吸附、致病基因的诱导、植物细胞DNA合成及修复的活力、外植体的状态等.最近的研究结果证明在适宜的条件下,根癌农杆菌还是可以有效地转化禾谷类作物.本文试就这两方面的研究进展作一论述.

Abstract: Agrobacterium tumefaciens mediated genetic transformation is the method most widely used in plant transformation.How to improve its transformation efficiency and extend its host range to include most cereals is what people concern about.There are many factors that influence the transformation effciency,including the wounding response of plants,attachment of bacteria,induction of virulent gene,DNA repair and replication activity of plant cells,state of explant,etc. Recent research has proved that cerelas can be transformed effectively by A.tumefaciens under suitable conditions.This paper reviewed the recent progress in the two aspects.

孔英珍, 周功克, 王根轩, 王亚馥. 影响根癌农杆菌转化的因素及其在单子叶作物上的应用[J]. 应用生态学报, 2000, (5): 791-794.

KONG Yingzhen, ZHOU Gongke, WANG Genxuan, WANG Yafu. Factors affecting transormation of Agrobacterium tumefaciens and their application on cereals[J]. Chinese Journal of Applied Ecology, 2000, (5): 791-794.

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