钩虾胆碱酯酶(ChE)和谷胱甘肽转硫酶(GST)的敏感性和特异性比较研究

应用生态学报 ›› 2001, Vol. 12 ›› Issue (4): 615-618.

钩虾胆碱酯酶(ChE)和谷胱甘肽转硫酶(GST)的敏感性和特异性比较研究

尹大强, 金洪钧, 于红霞, 陈良燕

南京大学环境学院污染控制与资源化研究国家重点实验室, 南京 210093

收稿日期:2000-06-05 修回日期:2000-09-11 出版日期:2001-07-25 发布日期:2001-07-25
通讯作者: 尹大强,男,1962年生,教授,主要从事生态毒理学方面的教学与研究工作,发表论文30余篇.Tel:025-3593262,E-mail:yindq@nju.edu.cn E-mail:yindq@nju.edu.cn
基金资助:
国家自然科学基金(39770153);中英合作资助项目

A comparative study on the sensitivity and specificity of cholinesterase and glutathione s-transferase in Gammarus pulex L

YIN Daqiang, JIN Hongjun, YU Hongxia, CHEN Liangyan

The State Lab of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093

Received:2000-06-05 Revised:2000-09-11 Online:2001-07-25 Published:2001-07-25

摘要/Abstract

摘要： 研究了有机磷农药甲基嘧啶硫磷、有机氯农药林丹、菊酯类农药氯菊酯、表面活性剂直链苯磺酸钠和重金属Zn对钩虾(Gammarus pulex L.)胆碱酯酶(ChE)和谷胱甘肽转硫酶(GST)活性变化以及毒性影响.结果表明,在暴露2h和8h后,仅有机磷农药甲基嘧啶硫磷显著抑制胆碱酯酶的活性;在暴露8h后,有机氯农药林丹和菊酯类农药氯菊酯能显著提高谷胱甘肽转硫酶活性,在暴露2h后,仅林丹导致谷胱甘肽转硫酶明显升高.作为生物标志物,胆碱酯酶比谷胱甘肽转硫酶具有更高的特异性;这两种生物标志物较毒性试验方法具有更高的敏感性.

Abstract: Studies on the influences of lindane,pirimiphos methyl,permethrin,zinc and dodecyl linear alkybenzene sulfonate(LAS)on the activity and toxicity of cholinesterase(ChE)and glutathione s-transferase(GST)in Gammarus pulex L. showed that only pirimiphos methyl caused a change in ChE activity in Gammarus,with a significant reduction in enzyme activity after²4h and 48h exposure.Both lindane and permethrin caused a change in GST activity in Gammarus,with a significant increase in enzyme activity after 48h exposure.Lindane alos caused a significant increase in GST activity after²4h exposure.Biomarkers ChE and GST were demonstrated a high degree of specificity and sensiti- vity in comparison to the lethality assay,but GST activity was less specific than ChE activity.

中图分类号:

X172

尹大强, 金洪钧, 于红霞, 陈良燕. 钩虾胆碱酯酶(ChE)和谷胱甘肽转硫酶(GST)的敏感性和特异性比较研究[J]. 应用生态学报, 2001, 12(4): 615-618.

YIN Daqiang, JIN Hongjun, YU Hongxia, CHEN Liangyan . A comparative study on the sensitivity and specificity of cholinesterase and glutathione s-transferase in Gammarus pulex L[J]. Chinese Journal of Applied Ecology, 2001, 12(4): 615-618.