恩诺沙星和铜复合污染对蚯蚓消化酶活性的影响

doi:10.13287/j.1001-9332.201906.034

应用生态学报 ›› 2019, Vol. 30 ›› Issue (6): 2049-2055.doi: 10.13287/j.1001-9332.201906.034

恩诺沙星和铜复合污染对蚯蚓消化酶活性的影响

张薇^1,2, 张萌¹, 陈凯¹, 金玉贺¹, 阎卉依¹, 胡艳美¹, 金兰淑^1,*

¹沈阳农业大学土地与环境学院, 沈阳 110866;
²农业部产地环境污染防控重点实验室/天津市农业环境与农产品安全重点实验室, 天津 300191

收稿日期:2018-07-26 出版日期:2019-06-15 发布日期:2019-06-15
通讯作者: * E-mail: ara168@163.com
作者简介:张薇,女,1980年生,博士,讲师. 主要从事土壤污染生态毒理学研究. E-mail: zhangweilm04@163.com
基金资助:
国家自然科学基金项目(41401573)、农业部重点实验室开放基金项目(16nybcdhj-5)和辽宁省大学生创新创业训练计划项目(201710157000013)资助

Effects of enrofloxacin and Cu combined pollution on the activities of digestive enzymes of earthworm in soil.

ZHANG Wei^1,2, ZHANG Meng¹, CHEN Kai¹, JIN Yu-he¹, YAN Hui-yi¹, HU Yan-mei¹, JIN Lan-shu^1,*

¹College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, China;
²Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, Ministry of Agriculture/Tianjin Key Laboratory of Agro-Environment and Safe-product, Tianjin 300191, China

Received:2018-07-26 Online:2019-06-15 Published:2019-06-15
Supported by:
This work was supported by the National Natural Science Foundation of China (41401573), Ministry of Agriculture Key Laboratory Open Fund (16nybcdhj-5), and Liaoning Province College Students Innovation and Entrepreneurship Training Program (201710157000013)

摘要/Abstract

摘要： 从农田土壤畜禽粪源污染的实际出发,以典型兽药抗生素恩诺沙星(ENR)和饲料添加剂铜(Cu)为污染物,研究了两污染物单一/复合暴露对蚯蚓的毒性效应.结果表明: 单一污染暴露下,恩诺沙星(0.1～4 mg·kg^-1,28 d)对蚯蚓蛋白酶未产生显著影响,对纤维素酶和碱性磷酸酶产生了抑制作用,而对酸性磷酸酶产生了诱导效应;铜(20～200 mg·kg^-1,28 d)对蚯蚓的蛋白酶、纤维素酶和磷酸酶活性总体均表现为抑制效应.复合污染暴露下,两污染物对蚯蚓消化酶的影响以抑制效应为主,且对纤维素酶和酸性磷酸酶的抑制表现出毒性增加的协同效应.消化酶随暴露时间的响应动态规律为: 调整性反应(3 d)-激烈反应(7 d)-反应平复(14 d)-慢性暴露(28 d).慢性暴露结果显示,含高剂量(200 mg·kg^-1 Cu或4 mg·kg^-1 ENR)污染物的复合组更具生态风险性.

Abstract: The effects of single and combined pollution of enrofloxacin and Cu on the digestive enzymes of earthworms were studied, based on the actual pollution of caused by the application of livestock feces in farmland soil. Results showed that single enrofloxacin (0.1-4 mg·kg^-1, 28 d) did not affect protease, but inhibited cellulase and alkaline phosphatase, with an induced effect on acid phosphatase. Single Cu pollution (20-200 mg·kg^-1, 28 d) had inhibitory effects on the four digestive enzymes in earthworms. The effects of combined exposures on the digestive enzymes were mainly negative, showing a synergistic increasing character of toxicity in cellulase and acid phosphatase activities. The response dynamics of digestive enzymes to exposure duration was regulatory response (3 d)-intense response (7 d)-reaction recovery (14 d)-chronic exposure (28 d). Chronic exposure results showed that the combined treatments containing high-dose pollutant (200 mg·kg^-1 Cu or 4 mg·kg^-1 ENR) had more ecological risk.

张薇, 张萌, 陈凯, 金玉贺, 阎卉依, 胡艳美, 金兰淑. 恩诺沙星和铜复合污染对蚯蚓消化酶活性的影响[J]. 应用生态学报, 2019, 30(6): 2049-2055.

ZHANG Wei, ZHANG Meng, CHEN Kai, JIN Yu-he, YAN Hui-yi, HU Yan-mei, JIN Lan-shu. Effects of enrofloxacin and Cu combined pollution on the activities of digestive enzymes of earthworm in soil.[J]. Chinese Journal of Applied Ecology, 2019, 30(6): 2049-2055.

参考文献

[1] Meng L (孟磊), Yang B (杨兵), Xue N-D (薛南冬). A review on environmental behaviors and ecoto-xicology of fluoroquinolone antibiotics. Asian Journal of Ecotoxicology (生态毒理学报), 2015, 10(2): 76-88 (in Chinese)
[2] Li W-M (李伟明), Bao Y-Y (鲍艳宇), Zhou Q-X (周启星). Degradation pathways and main degradation products of tetracycline antibiotics: Research progress. Chinese Journal of Applied Ecology (应用生态学报), 2012, 23(8): 2300-2308 (in Chinese)
[3] Chen X (陈璇), Guo X-F (郭雄飞), Chen G-G (陈桂葵), et al. Effects of biochar and pig manure on ipomoea aquatic forsk growth and copper forms in copper-polluted soil. Journal of Agro-Environment Science (农业环境科学学报), 2016, 35(5): 913-918 (in Chinese)
[4] Zhao L, Dong YH, Wang H. Residues of veterinary antibiotics in manures from feedlot livestock in eight provinces of China. Science of the Total Environment, 2010, 408: 1069-1075
[5] Zhang M-K (章明奎), Liu Z-Y (刘兆云). Effects of pig manure and oxytetracycline on microbial number and activity in soils with different fertility levels. Chinese Journal of Applied Ecology (应用生态学报), 2009, 20(11): 2790-2798 (in Chinese)
[6] Seedher N, Agarwal P. Effect of metal ions on some pharmacologically relevant interactions involving fluoroquinolone antibiotics. Drug Metabolism & Drug Interactions, 2010, 25: 17
[7] Wei Z-Y (魏子艳). Effects of Oxytetracycline, Enrofloxacin, Sulfamethazine and Copper Single and Combined Pollution on Soil Microbes. Master Thesis. Tai’an: Shandong Agricultural University, 2014 (in Chinese)
[8] Mao S-S (毛书帅). Effects of Single and Combined Pollution of Three Antibiotics and Copper on Functional Diversity of Soil Enzymes and Microbial Communities. Master Thesis. Tai’an: Shandong Agricultural University, 2016 (in Chinese)
[9] Li T(李通), Jin C-X (金彩霞), Zhu W-F (朱雯斐), et al. Joint toxicity of CPFX and Cu on seed germination and root elongation of corn, radish and Chinese cabbage. Journal of Agro-Environment Science (农业环境科学学报), 2013, 32(1): 15-20 (in Chinese)
[10] Ali U, Sajid N, Khalid A, et al. A review on vermicomposting of organic wastes. Environmental Progress & Sustainable Energy, 2015, 34: 1050-1062
[11] Whalen JK, Parmelee RW. Earthworm secondary production and N flux in agroecosystems: A comparison of two approaches. Oecologia, 2000, 124: 561-573
[12] Hoang DTT, Razavi BS, Kuzyakov Y, et al. Earthworm burrows: Kinetics and spatial distribution of enzymes of C-, N- and P-cycles. Soil Biology and Biochemistry, 2016, 99: 94-103
[13] Kuzyakov Y, Blagodatskaya E. Microbial hotspots and hot moments in soil: Concept & review. Soil Biology and Biochemistry, 2015, 83: 184-199
[14] Sampedro L, Whalen JK. Changes in the fatty acid profiles through the digestive tract of the earthworm Lumbricus terrestris L. Applied Soil Ecology, 2007, 35: 226-236
[15] Martinkosky L, Barkley J, Sabadell G, et al. Earthworms (Eisenia fetida) demonstrate potential for use in soil bioremediation by increasing the degradation rates of heavy crude oil hydrocarbons. Science of the Total Environment, 2017, 580: 734-743
[16] Yin L-J (尹丽君), Guo H (郭慧), Zhou N-Z (周乃珍), et al. Histochemical localization of seven digestive enzymes in different components of the alimentary canal of common earthworm Pheretima tschiliensis and its bearing on their function. Public Communication of Science & Technology (科技传播), 2011(3): 102-105 (in Chinese)
[17] Singh P, Mitra S, Majumdar D, et al. Nutrient and enzyme mobilization in earthworm casts: A comparative study with addition of selective amendments in undisturbed and agricultural soils of a mountain ecosystem. International Biodeterioration & Biodegradation, 2017, 119: 437-447
[18] Zhang W (张威), Zhang M (张明), Zhang X-D (张旭东), et al. A review on soil protease and arylamidase. Chinese Journal of Soil Science (土壤通报), 2008, 39(6): 1468-1474 (in Chinese)
[19] Park IY, Cha JR, Ok S, et al. A new earthworm cellulase and its possible role in the innate immunity. Deve-lopmental & Comparative Immunology, 2017, 67: 476-480
[20] Zhang B-G (张宝贵), Li G-T (李贵桐), Sun Z (孙钊), et al. Comparative study of digestive enzyme activities in earthworms belonging to two distinct ecological categories. Acta Ecologica Sinica (生态学报), 2001, 21(6): 978-981 (in Chinese)
[21] Zhang M (张敏), Xing Y-L (邢永雷), Li C-T (李成涛), et al. Effect of PBS-based copolymer on protein content and cellulose enzyme activity in earthworm. Ecology and Environmental Sciences (生态环境学报), 2012, 21(7): 1334-1338
[22] Mininstry of Ecology and Environment of the People’s Republic of China (中华人民共和国生态环境部). Soil Environmental Quality Risk Control Standard for Soil Contamination of Agricultural Land (GB 15618-2018). Beijing: China Standards Press, 2018 (in Chinese)
[23] Rost U, Joergensen RG, Chander K. Effects of Zn enriched sewage sludge on microbial activities and biomass in soil. Soil Biology & Biochemistry, 2001, 33: 633-638
[24] Renella G, Mench M, Lelie DVD, et al. Hydrolase activity, microbial biomass and community structure in long-term Cd-contaminated soils. Soil Biology & Biochemistry, 2007, 36: 443-451
[25] Zhang M-K (章明奎), Gu G-P (顾国平), Bao C-Y (鲍陈燕). Degradation characteristics of veterinary antibiotics in soils and its relationship with soil properties. Chinese Agricultural Science Bulletin (中国农学通报), 2015, 31(31): 228-236 (in Chinese)
[26] Gao M, Zhou Q, Song W, et al. Combined effects of oxytetracycline and Pb on earthworm Eisenia fetida. Environmental Toxicology & Pharmacology, 2014, 37: 689-696
[27] Cheng J-H (程菁恒), Zhu B-W (朱蓓薇), Wu H-T (吴海涛), et al. Extraction and characterization of crude alkaline phosphatase from the gut of sea cucumber Stichopus japonicus. Journal of Dalian Polytechnic University (大连工业大学学报), 2012, 31(2): 83-87 (in Chinese)

恩诺沙星和铜复合污染对蚯蚓消化酶活性的影响

Effects of enrofloxacin and Cu combined pollution on the activities of digestive enzymes of earthworm in soil.

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